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• W3119635134 endingPage "175" @default.
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• W3119635134 abstract "Single-cell technology is a powerful tool to dissect the heterogeneity and characteristics of rare cell populations during mammalian T cell development. T cell development in human fetal and postnatal development has been studied at the transcriptional level and at single-cell resolution. New insights regarding thymus seeding progenitors and early thymic progenitors have been unveiled using single-cell sequencing. The development of unconventional T cells and innate lymphoid cells continues to be deciphered and is aided by single-cell sequencing. Mammalian T cell development initiates from the migration of hematopoietic progenitors to the thymus, which undergo cell proliferation, T-lineage specification and commitment, as well as positive and negative selection. These processes are precisely controlled at multiple levels and have been intensively studied using gene-modified animal models and in vitro coculture systems. However, several long-standing questions, including the characterization of the rare but crucial progenitors/precursors and the molecular mechanisms underlying their fate decision, have been dampened because of cell scarcity and lack of appropriate techniques. Single-cell RNA sequencing (scRNA-seq) makes it possible to investigate and resolve some of these questions, leading to new remarkable progress in identifying and characterizing early thymic progenitors and delineating the refined developmental trajectories of conventional and unconventional T cells. Mammalian T cell development initiates from the migration of hematopoietic progenitors to the thymus, which undergo cell proliferation, T-lineage specification and commitment, as well as positive and negative selection. These processes are precisely controlled at multiple levels and have been intensively studied using gene-modified animal models and in vitro coculture systems. However, several long-standing questions, including the characterization of the rare but crucial progenitors/precursors and the molecular mechanisms underlying their fate decision, have been dampened because of cell scarcity and lack of appropriate techniques. Single-cell RNA sequencing (scRNA-seq) makes it possible to investigate and resolve some of these questions, leading to new remarkable progress in identifying and characterizing early thymic progenitors and delineating the refined developmental trajectories of conventional and unconventional T cells. droplet-based scRNA-seq platform. 3D in vitro system to test the lineage potential of a T cell progenitor or precursor. sequencing technique to access chromatin accessibility at the genome level. the checkpoint that occurs at the DN3 stage. Only thymocytes with a rearranged TCRβ forming the functional pre-TCR with pre-Tα pass the β-selection checkpoint, proliferate further, and develop into double-positive cells (CD4+CD8+). marker for hematopoietic and stem/progenitor cells. the most variable regions in the TCRs that determine their antigen specificity. technique to gain the transcriptional data and surface protein information of the same single cell. earliest intrathymic multipotent progenitors without a definitive T cell marker. known as type I or classical NKT cells that bear invariant αβTCR and NK cell markers (CD56 and CD161 in human and NK1.1 in mouse). subset of ILCs that are essential for lymphoid tissue development. enhanced lymphoid lineage potential of hematopoietic stem progenitor cells. T cells with semi-invariant αβTCR, which recognize public antigens presented by MHC-related molecular 1 (MR1). in vitro coculture systems using murine stromal cells (MS-5 cells) or (OP-9 cells) as feeder cells; used to test the lineage potential of stem cells. developing thymocytes with successfully rearranged TCRs undergo positive selection to select cells with self-MHC-responding TCRs and negative selection to deplete autoreactive thymocytes. when considering techniques such as scRNAseq, it refers to the process of reducing the dimensionality of large datasets by transforming them into smaller ones to increase interpretability while retaining the most of variation. conserved noncoding DNA sequences recognized by RAG1/RAG2 during TCR or B cell receptor rearrangement. sequencing technology enabling the identification of many RNA transcripts directly in single cells with their spatial context being preserved. specialized epithelial cells in the thymus that are divided into cortical TECs (cTEC) and medullary TECs (mTECs) based on anatomic and phenotypic profiles. hematopoietic progenitors in fetal liver and bone marrow that migrate to the thymus for T cell development. immunosuppressive CD4+ T cells expressing transcription factor FOXP3. having T cell lineage potential exclusively. the variable (V), diversity (D), and joining (J) gene segments of the TCR and B cell receptor gene. The recombination of V(D)J generates the highly diverse repertoire of TCRs and antibodies." @default.
• W3119635134 created "2021-01-18" @default.
• W3119635134 creator A5001660190 @default.
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• W3119635134 creator A5076655622 @default.
• W3119635134 date "2021-02-01" @default.
• W3119635134 modified "2023-10-16" @default.
• W3119635134 title "T Cell Development: Old Tales Retold By Single-Cell RNA Sequencing" @default.
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