Matches in SemOpenAlex for { <https://semopenalex.org/work/W3119694352> ?p ?o ?g. }
- W3119694352 endingPage "197" @default.
- W3119694352 startingPage "187" @default.
- W3119694352 abstract "Protein encapsulation into nanocarriers has been extensively studied to improve the efficacy and stability of therapeutic proteins. However, the chemical modification of proteins or new synthetic carrier materials are essential to achieve a high encapsulation efficiency and structural stability of proteins, which hinders their clinical applications. New strategies to physically incorporate proteins into nanocarriers feasible for clinical uses are required to overcome the current limitation. Here we report the spontaneous protein-induced reorganization of 'pre-formed' unilamellar lipid vesicles to efficiently incorporate proteins within multilamellar protein-lipid hybrid vesicles without chemical modification. Epidermal growth factor (EGF) binds to the surface of cationic unilamellar lipid vesicles and induces layer-by-layer self-assembly of the vesicles. The protein is spontaneously entrapped in the interstitial layers of a multilamellar structure with extremely high loading efficiency, ~99%, through polyionic interactions as predicted by molecular dynamics simulation. The loaded protein exhibits much higher structural, chemical, and biological stability compared to free protein. The method is also successfully applied to several other proteins. This work provides a promising method for the highly efficient encapsulation of therapeutic proteins into multilamellar lipid vesicles without the use of specialized instruments, high energy, coupling agents, or organic solvents." @default.
- W3119694352 created "2021-01-18" @default.
- W3119694352 creator A5002239807 @default.
- W3119694352 creator A5012227349 @default.
- W3119694352 creator A5013495284 @default.
- W3119694352 creator A5025201874 @default.
- W3119694352 creator A5027456707 @default.
- W3119694352 creator A5037303921 @default.
- W3119694352 creator A5040799331 @default.
- W3119694352 creator A5045084210 @default.
- W3119694352 creator A5045941705 @default.
- W3119694352 creator A5084687291 @default.
- W3119694352 date "2021-03-01" @default.
- W3119694352 modified "2023-10-03" @default.
- W3119694352 title "Protein-induced metamorphosis of unilamellar lipid vesicles to multilamellar hybrid vesicles" @default.
- W3119694352 cites W1543535234 @default.
- W3119694352 cites W1565510860 @default.
- W3119694352 cites W1584029749 @default.
- W3119694352 cites W1588613452 @default.
- W3119694352 cites W1596261235 @default.
- W3119694352 cites W1966078827 @default.
- W3119694352 cites W1967388315 @default.
- W3119694352 cites W1969791554 @default.
- W3119694352 cites W1971306482 @default.
- W3119694352 cites W1975346731 @default.
- W3119694352 cites W1976499671 @default.
- W3119694352 cites W1981425077 @default.
- W3119694352 cites W1982281628 @default.
- W3119694352 cites W1982914323 @default.
- W3119694352 cites W1983972373 @default.
- W3119694352 cites W1984111387 @default.
- W3119694352 cites W1984485373 @default.
- W3119694352 cites W1985516113 @default.
- W3119694352 cites W1985701428 @default.
- W3119694352 cites W1993816480 @default.
- W3119694352 cites W1994699094 @default.
- W3119694352 cites W1997446043 @default.
- W3119694352 cites W2003612288 @default.
- W3119694352 cites W2004178930 @default.
- W3119694352 cites W2004355032 @default.
- W3119694352 cites W2005124330 @default.
- W3119694352 cites W2006707210 @default.
- W3119694352 cites W2008632715 @default.
- W3119694352 cites W2008702713 @default.
- W3119694352 cites W2015881252 @default.
- W3119694352 cites W2016189767 @default.
- W3119694352 cites W2017765041 @default.
- W3119694352 cites W2022406746 @default.
- W3119694352 cites W2023906755 @default.
- W3119694352 cites W2025629542 @default.
- W3119694352 cites W2026551464 @default.
- W3119694352 cites W2029090560 @default.
- W3119694352 cites W2029604755 @default.
- W3119694352 cites W2031125344 @default.
- W3119694352 cites W2039773705 @default.
- W3119694352 cites W2041945932 @default.
- W3119694352 cites W2045101135 @default.
- W3119694352 cites W2047188856 @default.
- W3119694352 cites W2048927441 @default.
- W3119694352 cites W2051381895 @default.
- W3119694352 cites W2051516831 @default.
- W3119694352 cites W2054688134 @default.
- W3119694352 cites W2056223954 @default.
- W3119694352 cites W2065051465 @default.
- W3119694352 cites W2068723815 @default.
- W3119694352 cites W2071291987 @default.
- W3119694352 cites W2073080582 @default.
- W3119694352 cites W2073640246 @default.
- W3119694352 cites W2081449518 @default.
- W3119694352 cites W2085517327 @default.
- W3119694352 cites W2085672085 @default.
- W3119694352 cites W2093248644 @default.
- W3119694352 cites W2095930245 @default.
- W3119694352 cites W2114136530 @default.
- W3119694352 cites W2115030453 @default.
- W3119694352 cites W2116392598 @default.
- W3119694352 cites W2123585553 @default.
- W3119694352 cites W2150515351 @default.
- W3119694352 cites W2158273823 @default.
- W3119694352 cites W2168240189 @default.
- W3119694352 cites W2199052426 @default.
- W3119694352 cites W2313070179 @default.
- W3119694352 cites W2317805656 @default.
- W3119694352 cites W2323871038 @default.
- W3119694352 cites W2462494251 @default.
- W3119694352 cites W2510152670 @default.
- W3119694352 cites W2599276599 @default.
- W3119694352 cites W2612967995 @default.
- W3119694352 cites W2616586466 @default.
- W3119694352 cites W2747864271 @default.
- W3119694352 cites W2802572660 @default.
- W3119694352 cites W2969017294 @default.
- W3119694352 cites W2971912740 @default.
- W3119694352 cites W4246015058 @default.
- W3119694352 cites W758305415 @default.
- W3119694352 doi "https://doi.org/10.1016/j.jconrel.2021.01.004" @default.
- W3119694352 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33422501" @default.
- W3119694352 hasPublicationYear "2021" @default.