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• W3119783904 abstract "Short-range semiochemicals convey individual-specific information, which is important for final successful courtship and copulation. In this study, an electroantennography (EAG) instrument was used to determine the legs of male Bactrocera dorsalis that might participate in the perception of 4-allyl-2,6-dimethoxyphenol (4-DMP), a female-biased cuticular hydrocarbon (CHC) component. By performing comparative RNA-seq analysis, nine differentially expressed OBPs between 4-DMP-stimulated (M4-DMP) and unstimulated (M) male flies were screened out. Among the four downregulated OBPs, BdorOBP2 exhibited the strongest binding to 4-DMP than BdorOBP4, BdorOBP19a, and BdorOBP56h. Functional analyses confirmed that the reduction in transcript abundance of BdorOBP2 led to a significant decrease in behavioral responses of male flies to 4-DMP. In silico simulation revealed dramatic changes in the key residues and conformation between the two complexes, implying that BdorOBP2 might activate different receptors after binding to 4-DMP or methyl eugenol (ME). The results of this study suggest that BdorOBP2 mediates behavioral responses to 4-DMP and could be a promising molecular target for strategies of pest control." @default.
• W3119783904 created "2021-01-18" @default.
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• W3119783904 date "2021-01-04" @default.
• W3119783904 modified "2023-10-17" @default.
• W3119783904 title "OBP2 in the Midlegs of the Male <i>Bactrocera dorsalis</i> Is Involved in the Perception of the Female-Biased Sex Pheromone 4-Allyl-2,6-dimethoxyphenol" @default.
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• W3119783904 doi "https://doi.org/10.1021/acs.jafc.0c05945" @default.
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• W3119783904 hasPublicationYear "2021" @default.
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