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- W3119900818 abstract "Abstract Calcium (Ca 2+ ) signalling plays an indispensable role in dental pulp and dentin regeneration, but the Ca 2+ responses of human dental pulp stem cells (hDPSCs) stimulated with tricalcium silicate-based (TCS-based) dental biomaterials remains largely unexplored. The objective of the present study was to identify and correlate extracellular Ca 2+ concentration, intracellular Ca 2+ dynamics, pH, cytotoxicity, gene expression and mineralization ability of human dental pulp stem cells (hDPSCs) stimulated with two different TCS-based biomaterials: Biodentine and ProRoot white MTA. The hDPSCs were exposed to the biomaterials, brought in contact with the overlaying medium, with subsequent measurements of extracellular Ca 2+ and pH, and intracellular Ca 2+ changes. Messenger RNA expression (BGLAP, TGF-β, MMP1 and BMP2), cytotoxicity (MTT and TUNEL) and mineralization potential (Alizarin red and Von Kossa staining) were then evaluated. Biodentine released significantly more Ca 2+ in the α-MEM medium than ProRoot WMTA but this had no cytotoxic impact on hDPSCs. The larger Biodentine-linked Ca 2+ release resulted in altered intracellular Ca 2+ dynamics, which attained a higher maximum amplitude, faster rise time and increased area under the curve of the Ca 2+ changes compared to ProRoot WMTA. Experiments with intracellular Ca 2+ chelation, demonstrated that the biomaterial-triggered Ca 2+ dynamics affected stem cell-related gene expression, cellular differentiation and mineralization potential. In conclusion, biomaterial-specific Ca 2+ dynamics in hDPSCs determine differentiation and mineralization outcomes, with increased Ca 2+ dynamics enhancing mineralization." @default.
- W3119900818 created "2021-01-18" @default.
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- W3119900818 date "2021-01-12" @default.
- W3119900818 modified "2023-10-13" @default.
- W3119900818 title "The calcium dynamics of human dental pulp stem cells stimulated with tricalcium silicate-based cements determine their differentiation and mineralization outcome" @default.
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- W3119900818 doi "https://doi.org/10.1038/s41598-020-80096-5" @default.
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