Matches in SemOpenAlex for { <https://semopenalex.org/work/W3120024983> ?p ?o ?g. }
- W3120024983 endingPage "1433" @default.
- W3120024983 startingPage "1424" @default.
- W3120024983 abstract "Three-dimensional (3D) human induced pluripotent stem cell-derived engineered cardiac tissues (hiPSC-ECTs) have emerged as a promising alternative to two-dimensional hiPSC-cardiomyocyte monolayer systems because hiPSC-ECTs are a closer representation of endogenous cardiac tissues and more faithfully reflect the relevant cardiac pathophysiology. The ability to perform functional and molecular assessments using the same hiPSC-ECT construct would allow for more reliable correlation between observed functional performance and underlying molecular events, and thus is critically needed. Herein, for the first time, we have established an integrated method that permits sequential assessment of functional properties and top-down proteomics from the same single hiPSC-ECT construct. We quantitatively determined the differences in isometric twitch force and the sarcomeric proteoforms between two groups of hiPSC-ECTs that differed in the duration of time of 3D-ECT culture. Importantly, by using this integrated method we discovered a new and strong correlation between the measured contractile parameters and the phosphorylation levels of alpha-tropomyosin between the two groups of hiPSC-ECTs. The integration of functional assessments together with molecular characterization by top-down proteomics in the same hiPSC-ECT construct enables a holistic analysis of hiPSC-ECTs to accelerate their applications in disease modeling, cardiotoxicity, and drug discovery. Data are available via ProteomeXchange with identifier PXD022814." @default.
- W3120024983 created "2021-01-18" @default.
- W3120024983 creator A5008484294 @default.
- W3120024983 creator A5010789458 @default.
- W3120024983 creator A5034194011 @default.
- W3120024983 creator A5036323056 @default.
- W3120024983 creator A5039290981 @default.
- W3120024983 creator A5055083973 @default.
- W3120024983 creator A5055084338 @default.
- W3120024983 creator A5070053244 @default.
- W3120024983 creator A5072284526 @default.
- W3120024983 creator A5078892757 @default.
- W3120024983 creator A5082621591 @default.
- W3120024983 creator A5087464233 @default.
- W3120024983 date "2021-01-04" @default.
- W3120024983 modified "2023-10-13" @default.
- W3120024983 title "Functionally Integrated Top-Down Proteomics for Standardized Assessment of Human Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues" @default.
- W3120024983 cites W1568341953 @default.
- W3120024983 cites W1907511822 @default.
- W3120024983 cites W1970288681 @default.
- W3120024983 cites W2001249356 @default.
- W3120024983 cites W2012671659 @default.
- W3120024983 cites W2027069914 @default.
- W3120024983 cites W2045650908 @default.
- W3120024983 cites W2062298134 @default.
- W3120024983 cites W2078134123 @default.
- W3120024983 cites W2083599207 @default.
- W3120024983 cites W2089278516 @default.
- W3120024983 cites W2146482199 @default.
- W3120024983 cites W2158128644 @default.
- W3120024983 cites W2166305181 @default.
- W3120024983 cites W2338021849 @default.
- W3120024983 cites W2475449079 @default.
- W3120024983 cites W2547125981 @default.
- W3120024983 cites W2567937106 @default.
- W3120024983 cites W2607019129 @default.
- W3120024983 cites W2610144546 @default.
- W3120024983 cites W2766450105 @default.
- W3120024983 cites W2769766412 @default.
- W3120024983 cites W2786733298 @default.
- W3120024983 cites W2790214985 @default.
- W3120024983 cites W2796068395 @default.
- W3120024983 cites W2799848176 @default.
- W3120024983 cites W2901646806 @default.
- W3120024983 cites W2904725428 @default.
- W3120024983 cites W2907850070 @default.
- W3120024983 cites W2940688051 @default.
- W3120024983 cites W2970801181 @default.
- W3120024983 cites W2978210721 @default.
- W3120024983 cites W2982218778 @default.
- W3120024983 cites W3046987053 @default.
- W3120024983 cites W3047360653 @default.
- W3120024983 cites W3088010440 @default.
- W3120024983 cites W560863099 @default.
- W3120024983 doi "https://doi.org/10.1021/acs.jproteome.0c00830" @default.
- W3120024983 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7872211" @default.
- W3120024983 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33395532" @default.
- W3120024983 hasPublicationYear "2021" @default.
- W3120024983 type Work @default.
- W3120024983 sameAs 3120024983 @default.
- W3120024983 citedByCount "11" @default.
- W3120024983 countsByYear W31200249832021 @default.
- W3120024983 countsByYear W31200249832022 @default.
- W3120024983 countsByYear W31200249832023 @default.
- W3120024983 crossrefType "journal-article" @default.
- W3120024983 hasAuthorship W3120024983A5008484294 @default.
- W3120024983 hasAuthorship W3120024983A5010789458 @default.
- W3120024983 hasAuthorship W3120024983A5034194011 @default.
- W3120024983 hasAuthorship W3120024983A5036323056 @default.
- W3120024983 hasAuthorship W3120024983A5039290981 @default.
- W3120024983 hasAuthorship W3120024983A5055083973 @default.
- W3120024983 hasAuthorship W3120024983A5055084338 @default.
- W3120024983 hasAuthorship W3120024983A5070053244 @default.
- W3120024983 hasAuthorship W3120024983A5072284526 @default.
- W3120024983 hasAuthorship W3120024983A5078892757 @default.
- W3120024983 hasAuthorship W3120024983A5082621591 @default.
- W3120024983 hasAuthorship W3120024983A5087464233 @default.
- W3120024983 hasBestOaLocation W31200249832 @default.
- W3120024983 hasConcept C104317684 @default.
- W3120024983 hasConcept C107459253 @default.
- W3120024983 hasConcept C126322002 @default.
- W3120024983 hasConcept C145103041 @default.
- W3120024983 hasConcept C169760540 @default.
- W3120024983 hasConcept C2776694085 @default.
- W3120024983 hasConcept C2778233292 @default.
- W3120024983 hasConcept C41008148 @default.
- W3120024983 hasConcept C46111723 @default.
- W3120024983 hasConcept C54355233 @default.
- W3120024983 hasConcept C60644358 @default.
- W3120024983 hasConcept C70721500 @default.
- W3120024983 hasConcept C71924100 @default.
- W3120024983 hasConcept C86803240 @default.
- W3120024983 hasConceptScore W3120024983C104317684 @default.
- W3120024983 hasConceptScore W3120024983C107459253 @default.
- W3120024983 hasConceptScore W3120024983C126322002 @default.
- W3120024983 hasConceptScore W3120024983C145103041 @default.
- W3120024983 hasConceptScore W3120024983C169760540 @default.
- W3120024983 hasConceptScore W3120024983C2776694085 @default.