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- W3120129360 abstract "Our efforts have been focused on developing a self-contained and transportable capillary electrophoresis (CE) system with integrated electrochemical detection (ECD). The current prototype includes all necessary electrodes on-chip and utilizes miniaturized CE and ECD supporting electronics custom designed for this purpose. A novel microfabrication procedure was used to create recessed platinum electrodes and the CE/EC device from two patterned ultra-flat glass substrates. The electrodes in the bottom substrate were formed by a self-aligned etch and deposition technique followed by a photolithographic lift-off process. The microchannels (20 /spl mu/m deep/spl times/50 /spl mu/m wide [avg]) were chemically etched into the top substrate followed by thermal bonding to complete the microchip device. CE/EC experiments were performed using 0.02M phosphate buffer (pH 6), an analyte/buffer solution (2.2 mM dopamine, 2.3 mM catechol) and varying separation voltages (0-250 V) with a custom electronics unit interfaced to a laptop computer for data acquisition. Detection limits (S/N=3) were found to be 4.3 /spl mu/M for dopamine and 4.4 /spl mu/M for catechol. A linear detection response was observed up to 2200 /spl mu/M and 2300 /spl mu/M for dopamine and catechol, respectively." @default.
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- W3120129360 date "2002-06-02" @default.
- W3120129360 modified "2023-09-27" @default.
- W3120129360 title "Integration of On-Chip Electrochemical Detection in a Microfabricated Capillary Electrophoresis Device" @default.
- W3120129360 doi "https://doi.org/10.31438/trf.hh2002.77" @default.
- W3120129360 hasPublicationYear "2002" @default.
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