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- W3120147361 abstract "Abstract An organophosphorus (OP) insecticides degrading strain Lactobacillus plantarum WCP931, harboring OP hydrolase (OpdC) gene, was isolated during kimchi fermentation. The strain WCP931 appeared a significant survival rate of 51 to 96% under the artificial gastric acidic condition at pH 2 to 3 after 3 h. The opd C gene consisting 831 bp encoding 276 amino acids was cloned from the strain WCP907. The recombinant Escherischia coli harboring opd C gene depleted 77% chlorpyrifos (CP) in M9 medium after 6 days of incubation. The OpdC enzyme represents a novel member of GHSQG family of esterolytic enzymes or new Opd group. The OpdC molecular mass was estimated to be approximately 31 kDa in SDS-PAGE and showed maximum activity at 40 ο C with pH 6. However, the mutated OpdC (Ser116 → Ala116) enzyme had no activity towards OP insecticides and ρ -nitrophenol-β-butyrate. Importantly, relative activity of OpdC against P-O bond insecticides was higher than P-S bond insecticide, which indicated its broad substrate specificity. It is suggested that opd C gene of strain WCP931 play role for the biodegradation of OP insecticides during kimchi fermentation." @default.
- W3120147361 created "2021-01-18" @default.
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- W3120147361 date "2020-04-21" @default.
- W3120147361 modified "2023-09-27" @default.
- W3120147361 title "Cloning, expression, and functional characterization of an organophosphates insecticides degrading gene (opdC) from a potential probiotic Lactobacillus plantarum WCP931" @default.
- W3120147361 doi "https://doi.org/10.21203/rs.3.rs-21471/v1" @default.
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