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- W3120231314 abstract "Abstract Synthetic genomics‐driven dematerialization of genetic resources facilitates flexible hypothesis testing and rapid product development. Biological sequences have compositional biases, which, I reasoned, could be exploited for engineering of enhanced synthetic genomics systems. In proof‐of‐concept assays reported herein, the abundance of random oligonucleotides in viral genomic components was analyzed and used for the rational design of a synthetic genomics framework with plant virome capacity (SynViP). Type IIS endonucleases with low abundance in the plant virome, as well as Golden Gate and No See'm principles were combined with DNA chemical synthesis for seamless viral clone assembly by one‐step digestion‐ligation. The framework described does not require subcloning steps, is insensitive to insert terminal sequences, and was used with linear and circular DNA molecules. Based on a digital template, DNA fragments were chemically synthesized and assembled by one‐step cloning to yield a scar‐free infectious clone of a plant virus suitable for Agrobacterium ‐mediated delivery. SynViP allowed rescue of a genuine virus without biological material, and has the potential to greatly accelerate biological characterization and engineering of plant viruses as well as derived biotechnological tools. Finally, computational identification of compositional biases in biological sequences might become a common standard to aid scalable biosystems design and engineering." @default.
- W3120231314 created "2021-01-18" @default.
- W3120231314 creator A5051426072 @default.
- W3120231314 date "2021-02-11" @default.
- W3120231314 modified "2023-10-13" @default.
- W3120231314 title "Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity" @default.
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- W3120231314 cites W1967382476 @default.
- W3120231314 cites W1975464703 @default.
- W3120231314 cites W1976608804 @default.
- W3120231314 cites W1978802861 @default.
- W3120231314 cites W1983438661 @default.
- W3120231314 cites W2001674944 @default.
- W3120231314 cites W2016667239 @default.
- W3120231314 cites W2024182976 @default.
- W3120231314 cites W2029583307 @default.
- W3120231314 cites W2035680796 @default.
- W3120231314 cites W2044539089 @default.
- W3120231314 cites W2045071364 @default.
- W3120231314 cites W2062359823 @default.
- W3120231314 cites W2073936714 @default.
- W3120231314 cites W2104424333 @default.
- W3120231314 cites W2125985516 @default.
- W3120231314 cites W2127150062 @default.
- W3120231314 cites W2131887043 @default.
- W3120231314 cites W2137479668 @default.
- W3120231314 cites W2140211206 @default.
- W3120231314 cites W2142094441 @default.
- W3120231314 cites W2149174733 @default.
- W3120231314 cites W2154881379 @default.
- W3120231314 cites W2155758567 @default.
- W3120231314 cites W2171164830 @default.
- W3120231314 cites W2282841281 @default.
- W3120231314 cites W2318754754 @default.
- W3120231314 cites W2330245166 @default.
- W3120231314 cites W2336950589 @default.
- W3120231314 cites W2496527413 @default.
- W3120231314 cites W2561073732 @default.
- W3120231314 cites W2580973460 @default.
- W3120231314 cites W2583527348 @default.
- W3120231314 cites W2584960092 @default.
- W3120231314 cites W2606406253 @default.
- W3120231314 cites W2614268785 @default.
- W3120231314 cites W2616302079 @default.
- W3120231314 cites W2624387535 @default.
- W3120231314 cites W2724383252 @default.
- W3120231314 cites W2770740042 @default.
- W3120231314 cites W2789531943 @default.
- W3120231314 cites W2796415147 @default.
- W3120231314 cites W2799946861 @default.
- W3120231314 cites W2805277130 @default.
- W3120231314 cites W2892177242 @default.
- W3120231314 cites W2906721624 @default.
- W3120231314 cites W2913668923 @default.
- W3120231314 cites W2914085251 @default.
- W3120231314 cites W2919821266 @default.
- W3120231314 cites W2949223947 @default.
- W3120231314 cites W2958730175 @default.
- W3120231314 cites W2962481287 @default.
- W3120231314 cites W2965869024 @default.
- W3120231314 cites W2984666471 @default.
- W3120231314 cites W2986937238 @default.
- W3120231314 cites W2998410463 @default.
- W3120231314 cites W3004656082 @default.
- W3120231314 cites W3011062575 @default.
- W3120231314 cites W3011539579 @default.
- W3120231314 cites W3015625496 @default.
- W3120231314 cites W3015751519 @default.
- W3120231314 cites W3019939854 @default.
- W3120231314 cites W3033918756 @default.
- W3120231314 cites W3038729251 @default.
- W3120231314 cites W3041903330 @default.
- W3120231314 cites W3081989981 @default.
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- W3120231314 doi "https://doi.org/10.1002/biot.202000354" @default.
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- W3120231314 hasPublicationYear "2021" @default.
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