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• W3120272751 endingPage "230" @default.
• W3120272751 startingPage "220" @default.
• W3120272751 abstract "African swine fever (ASF) is an infectious disease caused by African swine fever virus (ASFV) with clinical symptoms of high fever, hemorrhages and high mortality rate, posing a threat to the global swine industry and food security. Quarantine and control of ASFV is crucial for preventing swine industry from ASFV infection. In this study, a recombinase polymerase amplification (RPA)-CRISPR-based nucleic acid detection method was developed for diagnosing ASF. As a highly sensitive method, RPA-CRISPR can detect even a single copy of ASFV plasmid and genomic DNA by determining fluorescence signal induced by collateral cleavage of CRISPR-lwCas13a (previously known as C2c2) through quantitative real-time PCR (qPCR) and has the same or even higher sensitivity than the traditional qPCR method. A lateral flow strip was developed and used in combination with RPA-CRISPR for ASFV detection with the same level of sensitivity of TaqMan qPCR. Likewise, RPA-CRISPR is capable of distinguishing ASFV genomic DNA from viral DNA/RNA of other porcine viruses without any cross-reactivity. This diagnostic method is also available for diagnosing ASFV clinical DNA samples with coincidence rate of 100% for both ASFV positive and negative samples. RPA-CRISPR has great potential for clinical quarantine of ASFV in swine industry and food security." @default.
• W3120272751 created "2021-01-18" @default.
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• W3120272751 date "2021-01-07" @default.
• W3120272751 modified "2023-09-30" @default.
• W3120272751 title "Development of A Super-Sensitive Diagnostic Method for African Swine Fever Using CRISPR Techniques" @default.
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• W3120272751 doi "https://doi.org/10.1007/s12250-020-00323-1" @default.
• W3120272751 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8087724" @default.
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• W3120272751 hasPublicationYear "2021" @default.
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