Matches in SemOpenAlex for { <https://semopenalex.org/work/W3120299968> ?p ?o ?g. }
- W3120299968 endingPage "22" @default.
- W3120299968 startingPage "13" @default.
- W3120299968 abstract "Glypican-3 (GPC3) is a highly specific diagnostic marker for hepatocellular carcinoma (HCC) diagnosis and a potential target in HCC therapy. Nanobodies (Nbs) are promising targeting molecules due to their high specificity and strong affinities to antigens, high stability, deep tissue penetration, and low immunogenicity. In this study, we isolated Nbs against GPC3 marker protein from a synthetic Nb library by phage display. To characterize these Nbs, we performed enzyme-linked immunosorbent assay, immunoprecipitation assay, and immunofluorescent assay to demonstrate that four (G8, G10, G11, and G64) of them bound specifically to recombinant as well as endogenous GPC3, and epitope mapping showed they all bound to N-terminal subunit of GPC3. Furthermore, we found that G64 exhibited high protein stability and GPC3 binding activity in serum at 37℃ for at least 96 h, and G64 did not affect the proliferation of HEK293T cells and HCC cell line HepG2. Our study provides four anti-GPC3 Nbs as promising targeting molecules for HCC diagnostic and therapeutic drugs." @default.
- W3120299968 created "2021-01-18" @default.
- W3120299968 creator A5022848854 @default.
- W3120299968 creator A5025937564 @default.
- W3120299968 creator A5040088181 @default.
- W3120299968 creator A5068163735 @default.
- W3120299968 date "2021-03-01" @default.
- W3120299968 modified "2023-09-28" @default.
- W3120299968 title "Identification of nanobodies against hepatocellular carcinoma marker glypican-3" @default.
- W3120299968 cites W1912512535 @default.
- W3120299968 cites W1964480542 @default.
- W3120299968 cites W1964538987 @default.
- W3120299968 cites W1967851963 @default.
- W3120299968 cites W1976784576 @default.
- W3120299968 cites W1978678628 @default.
- W3120299968 cites W1982878288 @default.
- W3120299968 cites W1989672802 @default.
- W3120299968 cites W2001927093 @default.
- W3120299968 cites W2005335309 @default.
- W3120299968 cites W2014041882 @default.
- W3120299968 cites W2014327275 @default.
- W3120299968 cites W2021048695 @default.
- W3120299968 cites W2028136261 @default.
- W3120299968 cites W2048879854 @default.
- W3120299968 cites W2058388773 @default.
- W3120299968 cites W2061630423 @default.
- W3120299968 cites W2074015830 @default.
- W3120299968 cites W2078592784 @default.
- W3120299968 cites W2079379157 @default.
- W3120299968 cites W2083669011 @default.
- W3120299968 cites W2084108774 @default.
- W3120299968 cites W2089788910 @default.
- W3120299968 cites W2091925589 @default.
- W3120299968 cites W2095553811 @default.
- W3120299968 cites W2098210920 @default.
- W3120299968 cites W2101562189 @default.
- W3120299968 cites W2106285087 @default.
- W3120299968 cites W21125662 @default.
- W3120299968 cites W2125900063 @default.
- W3120299968 cites W2126330431 @default.
- W3120299968 cites W2136704562 @default.
- W3120299968 cites W2138424758 @default.
- W3120299968 cites W2141468679 @default.
- W3120299968 cites W2151287838 @default.
- W3120299968 cites W2151305493 @default.
- W3120299968 cites W2160224841 @default.
- W3120299968 cites W2160360049 @default.
- W3120299968 cites W2169610187 @default.
- W3120299968 cites W2172145344 @default.
- W3120299968 cites W2272984102 @default.
- W3120299968 cites W2340240456 @default.
- W3120299968 cites W2487118445 @default.
- W3120299968 cites W2560322684 @default.
- W3120299968 cites W2580668003 @default.
- W3120299968 cites W2614367271 @default.
- W3120299968 cites W2615904899 @default.
- W3120299968 cites W2624710378 @default.
- W3120299968 cites W2746549636 @default.
- W3120299968 cites W2769445535 @default.
- W3120299968 cites W2780318634 @default.
- W3120299968 cites W2782890013 @default.
- W3120299968 cites W2787515413 @default.
- W3120299968 cites W2791721988 @default.
- W3120299968 cites W2797197823 @default.
- W3120299968 cites W2803358834 @default.
- W3120299968 cites W2889646458 @default.
- W3120299968 cites W2891129333 @default.
- W3120299968 cites W2898356184 @default.
- W3120299968 cites W2913862398 @default.
- W3120299968 cites W2942506747 @default.
- W3120299968 cites W2983258509 @default.
- W3120299968 cites W2983988502 @default.
- W3120299968 cites W3006578254 @default.
- W3120299968 cites W3013486203 @default.
- W3120299968 cites W3015865927 @default.
- W3120299968 cites W3041222256 @default.
- W3120299968 cites W3210430327 @default.
- W3120299968 cites W4229892776 @default.
- W3120299968 cites W4241399562 @default.
- W3120299968 doi "https://doi.org/10.1016/j.molimm.2021.01.010" @default.
- W3120299968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33453658" @default.
- W3120299968 hasPublicationYear "2021" @default.
- W3120299968 type Work @default.
- W3120299968 sameAs 3120299968 @default.
- W3120299968 citedByCount "7" @default.
- W3120299968 countsByYear W31202999682022 @default.
- W3120299968 countsByYear W31202999682023 @default.
- W3120299968 crossrefType "journal-article" @default.
- W3120299968 hasAuthorship W3120299968A5022848854 @default.
- W3120299968 hasAuthorship W3120299968A5025937564 @default.
- W3120299968 hasAuthorship W3120299968A5040088181 @default.
- W3120299968 hasAuthorship W3120299968A5068163735 @default.
- W3120299968 hasConcept C104292427 @default.
- W3120299968 hasConcept C104317684 @default.
- W3120299968 hasConcept C147483822 @default.
- W3120299968 hasConcept C153911025 @default.
- W3120299968 hasConcept C159654299 @default.
- W3120299968 hasConcept C185592680 @default.