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- W3120332800 abstract "Abstract The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition to base-pairing interactions between its guide RNA and target DNA. Previous reports have established that the PAM specificity of SpCas9 can be altered via positive selection procedures for directed evolution or other protein engineering strategies. Here we exploit in vivo directed evolution systems that incorporate simultaneous positive and negative selection to evolve SpCas9 variants with commensurate or improved activity on NAG PAMs relative to wild type and reduced activity on NGG PAMs, particularly YGG PAMs. We also show that the PAM preferences of available evolutionary intermediates effectively determine whether similar counterselection PAMs elicit different selection stringencies, and demonstrate that negative selection can be specifically increased in a yeast selection system through the fusion of compensatory zinc fingers to SpCas9." @default.
- W3120332800 created "2021-01-18" @default.
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- W3120332800 date "2021-01-13" @default.
- W3120332800 modified "2023-10-10" @default.
- W3120332800 title "Engineered dual selection for directed evolution of SpCas9 PAM specificity" @default.
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- W3120332800 doi "https://doi.org/10.1038/s41467-020-20650-x" @default.
- W3120332800 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7807044" @default.
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- W3120332800 hasPublicationYear "2021" @default.
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