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- W3120341290 abstract "<h2>ABSTRACT</h2> Our objective was to determine the effects of uncouplers of oxidative phosphorylation on the metabolism of propionate in liver tissue of dairy cows in the postpartum period. A total of 8 primiparous dairy cows were biopsied for liver tissue explants in 2 block-design experiments. Cows were 5.9 ± 2.8 (mean ± SD) days in milk, and the 2 experiments were run concurrently. Treatments for experiment 1 were 10 μ<i>M</i> 2,4-dinitrophenol methyl ether (DNPME) or propylene carbonate (vehicle control). Treatments for experiment 2 were 5 m<i>M</i> sodium salicylate (SAL) or no treatment (control). Explants were incubated in 2.5 m<i>M</i> [<sup>13</sup>C<sub>3</sub>]propionate with treatments and terminated after 0.5, 15, and 60 min of exposure to tracer. Treatment with DNPME had no effects on measured metabolites compared with control. Treatment with SAL increased total <sup>13</sup>C% enrichment of succinate (3.03 vs. 2.45%), but tended to decrease total <sup>13</sup>C% enrichment of fumarate (2.86 vs. 3.10%) and decreased total <sup>13</sup>C% enrichment of malate (3.96 vs. 4.58%) compared with the control. Treatment with DNPME appeared to have no effects on hepatic propionate metabolism, and treatment with SAL may impair the succinate dehydrogenase reaction." @default.
- W3120341290 created "2021-01-18" @default.
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- W3120341290 date "2021-03-01" @default.
- W3120341290 modified "2023-09-26" @default.
- W3120341290 title "Effect of uncouplers of oxidative phosphorylation on metabolism of propionate in liver explants from dairy cows" @default.
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- W3120341290 doi "https://doi.org/10.3168/jds.2020-19536" @default.
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