Matches in SemOpenAlex for { <https://semopenalex.org/work/W3120597914> ?p ?o ?g. }
- W3120597914 endingPage "72" @default.
- W3120597914 startingPage "61" @default.
- W3120597914 abstract "The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role of the electrospun fiber mat topography on neural stem cell (NSC) differentiation remains to be completely understood. In this work REN-VM cells (NSC model) were differentiated on polycaprolactone (PCL) nanofibers, obtained by wet/wet electrospinning, and on flat glass lamellas. The obtained differentiation profile of NSCs was evaluated using immunofluorescence and qPCR analysis. Glycosaminoglycan (GAG) analysis was successfully emplyed to evaluate changes in the GAG profile of differentiating cells through the use of the highly sensitive liquid chromatography-tandem mass/mass spectrometry (LC-MS/MS) method. Our results show that both culture platforms allow the differentiation of REN-VM cells into neural cells (neurons and astrocytes) similarly. Moreover, LC-MS/MS analysis shows changes in the production of GAGs present both in cell cultures and conditioned media samples. In the media, hyaluronic acid (HA) was detected and correlated with cellular activity and the production of a more plastic extracellular matrix. The cell samples evidence changes in chondroitin sulfate (CS4S, CS6S, CS4S6S) and heparan sulfate (HS6S, HS0S), similar to those previously described in vivo studies and possibly associated with the creation of complex structures, such as perineural networks. The GAG profile of differentiating REN-VM cells on electrospun scaffolds was analyzed for the first time. Our results highlight the advantage of using platforms obtain more reliable and robust neural tissue-engineered transplants." @default.
- W3120597914 created "2021-01-18" @default.
- W3120597914 creator A5005099065 @default.
- W3120597914 creator A5005526333 @default.
- W3120597914 creator A5007145023 @default.
- W3120597914 creator A5011326080 @default.
- W3120597914 creator A5012916254 @default.
- W3120597914 creator A5013859210 @default.
- W3120597914 creator A5050790167 @default.
- W3120597914 creator A5056210370 @default.
- W3120597914 creator A5062911756 @default.
- W3120597914 creator A5071688029 @default.
- W3120597914 creator A5075046317 @default.
- W3120597914 creator A5081070553 @default.
- W3120597914 creator A5083413036 @default.
- W3120597914 creator A5084793009 @default.
- W3120597914 date "2021-03-01" @default.
- W3120597914 modified "2023-10-01" @default.
- W3120597914 title "The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells" @default.
- W3120597914 cites W1136313859 @default.
- W3120597914 cites W1217626775 @default.
- W3120597914 cites W1511872533 @default.
- W3120597914 cites W1912213076 @default.
- W3120597914 cites W1939469511 @default.
- W3120597914 cites W1963918349 @default.
- W3120597914 cites W1968804320 @default.
- W3120597914 cites W1969444872 @default.
- W3120597914 cites W1976632391 @default.
- W3120597914 cites W1979674691 @default.
- W3120597914 cites W1979812031 @default.
- W3120597914 cites W1984418916 @default.
- W3120597914 cites W1988059567 @default.
- W3120597914 cites W1992009091 @default.
- W3120597914 cites W1997651801 @default.
- W3120597914 cites W2000453869 @default.
- W3120597914 cites W2004968496 @default.
- W3120597914 cites W2007582187 @default.
- W3120597914 cites W2013772471 @default.
- W3120597914 cites W2035222904 @default.
- W3120597914 cites W2043931405 @default.
- W3120597914 cites W2054677853 @default.
- W3120597914 cites W2065123875 @default.
- W3120597914 cites W2067379855 @default.
- W3120597914 cites W2070372044 @default.
- W3120597914 cites W2080529683 @default.
- W3120597914 cites W2088327864 @default.
- W3120597914 cites W2090185129 @default.
- W3120597914 cites W2108005154 @default.
- W3120597914 cites W2112485709 @default.
- W3120597914 cites W2136845216 @default.
- W3120597914 cites W2137502661 @default.
- W3120597914 cites W2138418853 @default.
- W3120597914 cites W2138977668 @default.
- W3120597914 cites W2149408556 @default.
- W3120597914 cites W2150976512 @default.
- W3120597914 cites W2152931372 @default.
- W3120597914 cites W2156862232 @default.
- W3120597914 cites W2158378300 @default.
- W3120597914 cites W2161659221 @default.
- W3120597914 cites W2218070520 @default.
- W3120597914 cites W2302418890 @default.
- W3120597914 cites W2315359895 @default.
- W3120597914 cites W2371814968 @default.
- W3120597914 cites W2530980570 @default.
- W3120597914 cites W2577967191 @default.
- W3120597914 cites W2593719298 @default.
- W3120597914 cites W2602055249 @default.
- W3120597914 cites W2655218353 @default.
- W3120597914 cites W2734706909 @default.
- W3120597914 cites W2736382445 @default.
- W3120597914 cites W2754967293 @default.
- W3120597914 cites W2763819924 @default.
- W3120597914 cites W2770567970 @default.
- W3120597914 cites W2780962309 @default.
- W3120597914 cites W2781499621 @default.
- W3120597914 cites W2794377970 @default.
- W3120597914 cites W2807291660 @default.
- W3120597914 cites W2886445495 @default.
- W3120597914 cites W2898075408 @default.
- W3120597914 cites W2903926647 @default.
- W3120597914 cites W2905607681 @default.
- W3120597914 cites W2909619835 @default.
- W3120597914 cites W2911030495 @default.
- W3120597914 cites W2911893549 @default.
- W3120597914 cites W2914969344 @default.
- W3120597914 cites W2939689184 @default.
- W3120597914 cites W2942683738 @default.
- W3120597914 cites W2963630514 @default.
- W3120597914 cites W2976670407 @default.
- W3120597914 cites W2980047430 @default.
- W3120597914 cites W2982694954 @default.
- W3120597914 cites W2999176774 @default.
- W3120597914 cites W3008252616 @default.
- W3120597914 cites W3034149287 @default.
- W3120597914 cites W3035117084 @default.
- W3120597914 cites W3040017679 @default.
- W3120597914 cites W3096844512 @default.
- W3120597914 cites W3097867616 @default.