FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3120603606> ?p ?o ?g. }

• W3120603606 endingPage "624" @default.
• W3120603606 startingPage "624" @default.
• W3120603606 abstract "Endothelial cells and astrocytes preferentially express metabotropic P2Y nucleotide receptors, which are involved in the maintenance of vascular and neural function. Among these, P2Y1 and P2Y2 receptors appear as main actors, since their stimulation induces intracellular calcium mobilization and activates signaling cascades linked to cytoskeletal reorganization. In the present work, we have analyzed, by means of atomic force microscopy (AFM) in force spectroscopy mode, the mechanical response of human umbilical vein endothelial cells (HUVEC) and astrocytes upon 2MeSADP and UTP stimulation. This approach allows for simultaneous measurement of variations in factors such as Young’s modulus, maximum adhesion force and rupture event formation, which reflect the potential changes in both the stiffness and adhesiveness of the plasma membrane. The largest effect was observed in both endothelial cells and astrocytes after P2Y2 receptor stimulation with UTP. Such exposure to UTP doubled the Young’s modulus and reduced both the adhesion force and the number of rupture events. In astrocytes, 2MeSADP stimulation also had a remarkable effect on AFM parameters. Additional studies performed with the selective P2Y1 and P2Y13 receptor antagonists revealed that the 2MeSADP-induced mechanical changes were mediated by the P2Y13 receptor, although they were negatively modulated by P2Y1 receptor stimulation. Hence, our results demonstrate that AFM can be a very useful tool to evaluate functional native nucleotide receptors in living cells." @default.
• W3120603606 created "2021-01-18" @default.
• W3120603606 creator A5000070721 @default.
• W3120603606 creator A5003375625 @default.
• W3120603606 creator A5008258219 @default.
• W3120603606 creator A5021613768 @default.
• W3120603606 creator A5044519676 @default.
• W3120603606 creator A5050109617 @default.
• W3120603606 creator A5066317529 @default.
• W3120603606 creator A5084888586 @default.
• W3120603606 date "2021-01-10" @default.
• W3120603606 modified "2023-10-17" @default.
• W3120603606 title "Nucleotides-Induced Changes in the Mechanical Properties of Living Endothelial Cells and Astrocytes, Analyzed by Atomic Force Microscopy" @default.
• W3120603606 cites W1590390041 @default.
• W3120603606 cites W1947551305 @default.
• W3120603606 cites W1963813577 @default.
• W3120603606 cites W1980326204 @default.
• W3120603606 cites W1987710461 @default.
• W3120603606 cites W1992773620 @default.
• W3120603606 cites W1997875091 @default.
• W3120603606 cites W2003618420 @default.
• W3120603606 cites W2009649607 @default.
• W3120603606 cites W2011987393 @default.
• W3120603606 cites W2016892258 @default.
• W3120603606 cites W2018863750 @default.
• W3120603606 cites W2022107983 @default.
• W3120603606 cites W2035907673 @default.
• W3120603606 cites W2042744781 @default.
• W3120603606 cites W2045782182 @default.
• W3120603606 cites W2056358535 @default.
• W3120603606 cites W2066063160 @default.
• W3120603606 cites W2066367871 @default.
• W3120603606 cites W2071334288 @default.
• W3120603606 cites W2076444759 @default.
• W3120603606 cites W2084998933 @default.
• W3120603606 cites W2085533582 @default.
• W3120603606 cites W2086394203 @default.
• W3120603606 cites W2090296265 @default.
• W3120603606 cites W2094737861 @default.
• W3120603606 cites W2100452437 @default.
• W3120603606 cites W2110147488 @default.
• W3120603606 cites W2118067026 @default.
• W3120603606 cites W2122131535 @default.
• W3120603606 cites W2125649895 @default.
• W3120603606 cites W2129818296 @default.
• W3120603606 cites W2136008196 @default.
• W3120603606 cites W2146644233 @default.
• W3120603606 cites W2162008357 @default.
• W3120603606 cites W2172084977 @default.
• W3120603606 cites W2281343493 @default.
• W3120603606 cites W2468991623 @default.
• W3120603606 cites W2521899113 @default.
• W3120603606 cites W2524677258 @default.
• W3120603606 cites W2561499578 @default.
• W3120603606 cites W2727590480 @default.
• W3120603606 cites W2746047719 @default.
• W3120603606 cites W2768623326 @default.
• W3120603606 cites W2777960255 @default.
• W3120603606 cites W2782908979 @default.
• W3120603606 cites W2797319161 @default.
• W3120603606 cites W2899765970 @default.
• W3120603606 cites W2900899823 @default.
• W3120603606 cites W2905640208 @default.
• W3120603606 cites W2942077979 @default.
• W3120603606 cites W2946512789 @default.
• W3120603606 cites W2954817158 @default.
• W3120603606 cites W2968343115 @default.
• W3120603606 cites W2980735427 @default.
• W3120603606 cites W2993450133 @default.
• W3120603606 cites W3005154746 @default.
• W3120603606 cites W3021900117 @default.
• W3120603606 cites W3097463406 @default.
• W3120603606 doi "https://doi.org/10.3390/ijms22020624" @default.
• W3120603606 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7827192" @default.
• W3120603606 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33435130" @default.
• W3120603606 hasPublicationYear "2021" @default.
• W3120603606 type Work @default.
• W3120603606 sameAs 3120603606 @default.
• W3120603606 citedByCount "5" @default.
• W3120603606 countsByYear W31206036062021 @default.
• W3120603606 countsByYear W31206036062022 @default.
• W3120603606 crossrefType "journal-article" @default.
• W3120603606 hasAuthorship W3120603606A5000070721 @default.
• W3120603606 hasAuthorship W3120603606A5003375625 @default.
• W3120603606 hasAuthorship W3120603606A5008258219 @default.
• W3120603606 hasAuthorship W3120603606A5021613768 @default.
• W3120603606 hasAuthorship W3120603606A5044519676 @default.
• W3120603606 hasAuthorship W3120603606A5050109617 @default.
• W3120603606 hasAuthorship W3120603606A5066317529 @default.
• W3120603606 hasAuthorship W3120603606A5084888586 @default.
• W3120603606 hasBestOaLocation W31206036061 @default.
• W3120603606 hasConcept C102951782 @default.
• W3120603606 hasConcept C119473381 @default.
• W3120603606 hasConcept C12554922 @default.
• W3120603606 hasConcept C160225129 @default.
• W3120603606 hasConcept C169760540 @default.
• W3120603606 hasConcept C170493617 @default.
• W3120603606 hasConcept C171250308 @default.

• next