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• W3120708703 abstract "Finding out how cells prepare for fate change during differentiation commitment was our task. To address whether the constitutive pericentromere-associated domains (PADs) may be involved, we used a model system with known transcriptome data, MCF-7 breast cancer cells treated with the ErbB3 ligand heregulin (HRG), which induces differentiation and is used in the therapy of cancer. PAD-repressive heterochromatin (H3K9me3), centromere-associated-protein-specific, and active euchromatin (H3K4me3) antibodies, real-time PCR, acridine orange DNA structural test (AOT), and microscopic image analysis were applied. We found a two-step DNA unfolding after 15-20 and 60 min of HRG treatment, respectively. This behavior was consistent with biphasic activation of the early response genes (c-fos - fosL1/myc) and the timing of two transcriptome avalanches reported in the literature. In control, the average number of PADs negatively correlated with their size by scale-free distribution, and centromere clustering in turn correlated with PAD size, both indicating that PADs may create and modulate a suprachromosomal network by fusing and splitting a constant proportion of the constitutive heterochromatin. By 15 min of HRG treatment, the bursting unraveling of PADs from the nucleolus boundary occurred, coinciding with the first step of H3K4me3 chromatin unfolding, confirmed by AOT. The second step after 60 min of HRG treatment was associated with transcription of long noncoding RNA from PADs and peaking of fosL1/c-myc response. We hypothesize that the bursting of PAD clusters under a critical silencing threshold pushes the first transcription avalanche, whereas the destruction of the PAD network enables genome rewiring needed for differentiation repatterning, mediated by early response bivalent genes." @default.
• W3120708703 created "2021-01-18" @default.
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• W3120708703 date "2021-02-01" @default.
• W3120708703 modified "2023-09-25" @default.
• W3120708703 title "Differentiating cancer cells reveal early large-scale genome regulation by pericentric domains" @default.
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• W3120708703 doi "https://doi.org/10.1016/j.bpj.2021.01.002" @default.
• W3120708703 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7896032" @default.
• W3120708703 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33453273" @default.
• W3120708703 hasPublicationYear "2021" @default.
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