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- W3120715541 abstract "Spo11, which makes DNA double-strand breaks (DSBs) that are essential for meiotic recombination, has long been recalcitrant to biochemical study. We provide molecular analysis of Saccharomyces cerevisiae Spo11 purified with partners Rec102, Rec104 and Ski8. Rec102 and Rec104 jointly resemble the B subunit of archaeal topoisomerase VI, with Rec104 occupying a position similar to the Top6B GHKL-type ATPase domain. Unexpectedly, the Spo11 complex is monomeric (1:1:1:1 stoichiometry), consistent with dimerization controlling DSB formation. Reconstitution of DNA binding reveals topoisomerase-like preferences for duplex-duplex junctions and bent DNA. Spo11 also binds noncovalently but with high affinity to DNA ends mimicking cleavage products, suggesting a mechanism to cap DSB ends. Mutations that reduce DNA binding in vitro attenuate DSB formation, alter DSB processing and reshape the DSB landscape in vivo. Our data reveal structural and functional similarities between the Spo11 core complex and Topo VI, but also highlight differences reflecting their distinct biological roles." @default.
- W3120715541 created "2021-01-18" @default.
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- W3120715541 date "2021-01-01" @default.
- W3120715541 modified "2023-10-06" @default.
- W3120715541 title "Structural and functional characterization of the Spo11 core complex" @default.
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- W3120715541 doi "https://doi.org/10.1038/s41594-020-00534-w" @default.
- W3120715541 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7855791" @default.
- W3120715541 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33398171" @default.
- W3120715541 hasPublicationYear "2021" @default.
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