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- W3120865462 abstract "Abstract Megabodies are engineered nanobodies that help overcome two major obstacles that limit the resolution of single-particle cryo-EM reconstructions: particle size and preferential orientation at the water-air interfaces. Here we describe how nanobodies can be rigidly grafted onto selected protein scaffolds (HopQ or YgjK) to increase their molecular weight while retaining the full antigen binding specificity and affinity. We also describe the protocols to purify these chimeric molecules from the periplasm of E. coli ." @default.
- W3120865462 created "2021-01-18" @default.
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- W3120865462 date "2021-01-07" @default.
- W3120865462 modified "2023-09-25" @default.
- W3120865462 title "Constructing and purifying megabodies starting from individual nanobody sequences" @default.
- W3120865462 doi "https://doi.org/10.21203/rs.3.pex-1033/v1" @default.
- W3120865462 hasPublicationYear "2021" @default.
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