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- W3120907105 abstract "The development of a safe and effective SARS-CoV-2 vaccine is a public health priority. We designed subunit vaccine candidates using self-assembling ferritin nanoparticles displaying one of two multimerized SARS-CoV-2 spikes: full-length ectodomain (S-Fer) or a C-terminal 70 amino-acid deletion (SΔC-Fer). Ferritin is an attractive nanoparticle platform for production of vaccines, and ferritin-based vaccines have been investigated in humans in two separate clinical trials. We confirmed proper folding and antigenicity of spike on the surface of ferritin by cryo-EM and binding to conformation-specific monoclonal antibodies. After a single immunization of mice with either of the two spike ferritin particles, a lentiviral SARS-CoV-2 pseudovirus assay revealed mean neutralizing antibody titers at least 2-fold greater than those in convalescent plasma from COVID-19 patients. Additionally, a single dose of SΔC-Fer elicited significantly higher neutralizing responses as compared to immunization with the spike receptor binding domain (RBD) monomer or spike ectodomain trimer alone. After a second dose, mice immunized with SΔC-Fer exhibited higher neutralizing titers than all other groups. Taken together, these results demonstrate that multivalent presentation of SARS-CoV-2 spike on ferritin can notably enhance elicitation of neutralizing antibodies, thus constituting a viable strategy for single-dose vaccination against COVID-19." @default.
- W3120907105 created "2021-01-18" @default.
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- W3120907105 date "2021-01-05" @default.
- W3120907105 modified "2023-10-14" @default.
- W3120907105 title "A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice" @default.
- W3120907105 cites W1557780170 @default.
- W3120907105 cites W1816587284 @default.
- W3120907105 cites W1976136842 @default.
- W3120907105 cites W2002844537 @default.
- W3120907105 cites W2029555174 @default.
- W3120907105 cites W2035269438 @default.
- W3120907105 cites W2036949898 @default.
- W3120907105 cites W2055017188 @default.
- W3120907105 cites W2063074246 @default.
- W3120907105 cites W2067061290 @default.
- W3120907105 cites W2095278521 @default.
- W3120907105 cites W2095633747 @default.
- W3120907105 cites W2100455255 @default.
- W3120907105 cites W2104234755 @default.
- W3120907105 cites W2106995102 @default.
- W3120907105 cites W2110572977 @default.
- W3120907105 cites W2120679406 @default.
- W3120907105 cites W2123909340 @default.
- W3120907105 cites W2132629607 @default.
- W3120907105 cites W2161428851 @default.
- W3120907105 cites W2162802735 @default.
- W3120907105 cites W2165220571 @default.
- W3120907105 cites W2172319351 @default.
- W3120907105 cites W2174836001 @default.
- W3120907105 cites W2274022023 @default.
- W3120907105 cites W2276335432 @default.
- W3120907105 cites W2281676466 @default.
- W3120907105 cites W2292700442 @default.
- W3120907105 cites W2339647820 @default.
- W3120907105 cites W2469710699 @default.
- W3120907105 cites W2568896114 @default.
- W3120907105 cites W2587625522 @default.
- W3120907105 cites W2593511418 @default.
- W3120907105 cites W2711033291 @default.
- W3120907105 cites W2750464792 @default.
- W3120907105 cites W2953320394 @default.
- W3120907105 cites W3006703916 @default.
- W3120907105 cites W3007257464 @default.
- W3120907105 cites W3007643904 @default.
- W3120907105 cites W3009335299 @default.
- W3120907105 cites W3010441732 @default.
- W3120907105 cites W3010780802 @default.
- W3120907105 cites W3011537081 @default.
- W3120907105 cites W3013800395 @default.
- W3120907105 cites W3013855790 @default.
- W3120907105 cites W3014067025 @default.
- W3120907105 cites W3014656992 @default.
- W3120907105 cites W3015371627 @default.
- W3120907105 cites W3017302293 @default.
- W3120907105 cites W3020828664 @default.
- W3120907105 cites W3022906021 @default.
- W3120907105 cites W3022917947 @default.
- W3120907105 cites W3023376619 @default.
- W3120907105 cites W3023671369 @default.
- W3120907105 cites W3025521550 @default.
- W3120907105 cites W3025631553 @default.
- W3120907105 cites W3032255042 @default.
- W3120907105 cites W3034173374 @default.
- W3120907105 cites W3034425032 @default.
- W3120907105 cites W3035459690 @default.
- W3120907105 cites W3036386821 @default.
- W3120907105 cites W3036529319 @default.
- W3120907105 cites W3036668249 @default.
- W3120907105 cites W3040017923 @default.
- W3120907105 cites W3042833857 @default.
- W3120907105 cites W3044401827 @default.
- W3120907105 cites W3045724532 @default.
- W3120907105 cites W3046061621 @default.
- W3120907105 cites W3046416848 @default.
- W3120907105 cites W3046985131 @default.
- W3120907105 cites W3047091198 @default.
- W3120907105 cites W3048620154 @default.
- W3120907105 cites W3049142173 @default.
- W3120907105 cites W3058836816 @default.
- W3120907105 cites W3087845968 @default.
- W3120907105 cites W3087924281 @default.
- W3120907105 cites W3091443388 @default.
- W3120907105 cites W3091814234 @default.
- W3120907105 cites W3093367571 @default.
- W3120907105 cites W3094524617 @default.
- W3120907105 cites W3094860119 @default.
- W3120907105 cites W3103021984 @default.
- W3120907105 cites W3109969518 @default.