Matches in SemOpenAlex for { <https://semopenalex.org/work/W3121004564> ?p ?o ?g. }
- W3121004564 endingPage "968" @default.
- W3121004564 startingPage "954" @default.
- W3121004564 abstract "Abstract Catalytically inactive Cas9 (dCas9) has become an increasingly popular tool for targeted gene activation/inactivation, live-cell imaging, and base editing. While dCas9 was reported to induce base substitutions and indels, it has not been associated with structural variations. Here, we show that dCas9 impedes replication fork progression to destabilize tandem repeats in budding yeast. When targeted to the CUP1 array comprising ∼16 repeat units, dCas9 induced its contraction in most cells, especially in the presence of nicotinamide. Replication intermediate analysis demonstrated replication fork stalling in the vicinity of dCas9-bound sites. Genetic analysis indicated that while destabilization is counteracted by the replisome progression complex components Ctf4 and Mrc1 and the accessory helicase Rrm3, it involves single-strand annealing by the recombination proteins Rad52 and Rad59. Although dCas9-mediated replication fork stalling is a potential risk in conventional applications, it may serve as a novel tool for both mechanistic studies and manipulation of genomic instability." @default.
- W3121004564 created "2021-01-18" @default.
- W3121004564 creator A5007606729 @default.
- W3121004564 creator A5016307830 @default.
- W3121004564 creator A5017389109 @default.
- W3121004564 creator A5030648728 @default.
- W3121004564 creator A5035470984 @default.
- W3121004564 creator A5068543044 @default.
- W3121004564 creator A5074541984 @default.
- W3121004564 creator A5076238710 @default.
- W3121004564 date "2021-01-04" @default.
- W3121004564 modified "2023-10-17" @default.
- W3121004564 title "Catalytically inactive Cas9 impairs DNA replication fork progression to induce focal genomic instability" @default.
- W3121004564 cites W1521785965 @default.
- W3121004564 cites W1569227072 @default.
- W3121004564 cites W1604458367 @default.
- W3121004564 cites W1964727634 @default.
- W3121004564 cites W1975001355 @default.
- W3121004564 cites W1979914496 @default.
- W3121004564 cites W1981958246 @default.
- W3121004564 cites W1986559385 @default.
- W3121004564 cites W2006377598 @default.
- W3121004564 cites W2008486131 @default.
- W3121004564 cites W2013612878 @default.
- W3121004564 cites W2016038582 @default.
- W3121004564 cites W2020715816 @default.
- W3121004564 cites W2029084348 @default.
- W3121004564 cites W2033587361 @default.
- W3121004564 cites W2043257897 @default.
- W3121004564 cites W2046137036 @default.
- W3121004564 cites W2046358187 @default.
- W3121004564 cites W2053259460 @default.
- W3121004564 cites W2054524640 @default.
- W3121004564 cites W2058401582 @default.
- W3121004564 cites W2061683926 @default.
- W3121004564 cites W2066088066 @default.
- W3121004564 cites W2066780597 @default.
- W3121004564 cites W2068393186 @default.
- W3121004564 cites W2068427957 @default.
- W3121004564 cites W2079924690 @default.
- W3121004564 cites W2080862728 @default.
- W3121004564 cites W2089841991 @default.
- W3121004564 cites W2101749257 @default.
- W3121004564 cites W2103255598 @default.
- W3121004564 cites W2113551110 @default.
- W3121004564 cites W2115383745 @default.
- W3121004564 cites W2115420193 @default.
- W3121004564 cites W2117251080 @default.
- W3121004564 cites W2125321557 @default.
- W3121004564 cites W2129497695 @default.
- W3121004564 cites W2136998622 @default.
- W3121004564 cites W2141434167 @default.
- W3121004564 cites W2145782793 @default.
- W3121004564 cites W2146503498 @default.
- W3121004564 cites W2158387328 @default.
- W3121004564 cites W2160051211 @default.
- W3121004564 cites W2166459599 @default.
- W3121004564 cites W2170822302 @default.
- W3121004564 cites W2287379459 @default.
- W3121004564 cites W2414808421 @default.
- W3121004564 cites W2461013690 @default.
- W3121004564 cites W2494243053 @default.
- W3121004564 cites W2604463717 @default.
- W3121004564 cites W2614821800 @default.
- W3121004564 cites W2624483531 @default.
- W3121004564 cites W2694546118 @default.
- W3121004564 cites W2769910569 @default.
- W3121004564 cites W2854373346 @default.
- W3121004564 cites W2883697525 @default.
- W3121004564 cites W2890644736 @default.
- W3121004564 cites W2894310390 @default.
- W3121004564 cites W2906523411 @default.
- W3121004564 cites W2915013877 @default.
- W3121004564 cites W2919006054 @default.
- W3121004564 cites W2944977287 @default.
- W3121004564 cites W2951278111 @default.
- W3121004564 cites W2973731348 @default.
- W3121004564 cites W2975018492 @default.
- W3121004564 cites W3006859342 @default.
- W3121004564 cites W3008857052 @default.
- W3121004564 cites W3019896571 @default.
- W3121004564 cites W318278602 @default.
- W3121004564 doi "https://doi.org/10.1093/nar/gkaa1241" @default.
- W3121004564 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7826275" @default.
- W3121004564 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33398345" @default.
- W3121004564 hasPublicationYear "2021" @default.
- W3121004564 type Work @default.
- W3121004564 sameAs 3121004564 @default.
- W3121004564 citedByCount "23" @default.
- W3121004564 countsByYear W31210045642021 @default.
- W3121004564 countsByYear W31210045642022 @default.
- W3121004564 countsByYear W31210045642023 @default.
- W3121004564 crossrefType "journal-article" @default.
- W3121004564 hasAuthorship W3121004564A5007606729 @default.
- W3121004564 hasAuthorship W3121004564A5016307830 @default.
- W3121004564 hasAuthorship W3121004564A5017389109 @default.
- W3121004564 hasAuthorship W3121004564A5030648728 @default.
- W3121004564 hasAuthorship W3121004564A5035470984 @default.