FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3121004677> ?p ?o ?g. }

• W3121004677 endingPage "107" @default.
• W3121004677 startingPage "107" @default.
• W3121004677 abstract "Rapeseed oil-extracted expeller cake mostly contains protein. Various approaches have been used to isolate, detect and measure proteins in rapeseeds, with a particular focus on seed storage proteins (SSPs). To maximize the protein yield and minimize hazardous chemical use, isolation costs and the loss of seed material, optimization of the extraction method is pivotal. For some studies, it is also necessary to minimize or avoid seed-to-seed cross-contamination for phenotyping and single-tissue type analysis to know the exact amount of any bioactive component in a single seed, rather than a mixture of multiple seeds. However, a simple and robust method for single rapeseed seed protein extraction (SRPE) is unavailable. To establish a strategy for optimizing SRPE for downstream gel-based protein analysis, yielding the highest amount of SSPs in the most economical and rapid way, a variety of different approaches were tested, including variations to the seed pulverization steps, changes to the compositions of solvents and reagents and adjustments to the protein recovery steps. Following SRPE, 1D-SDS-PAGE was used to assess the quality and amount of proteins extracted. A standardized SRPE procedure was developed and then tested for yield and reproducibility. The highest protein yield and quality were obtained using a ball grinder with stainless steel beads in Safe-Lock microcentrifuge tubes with methanol as the solvent, providing a highly efficient, economic and effective method. The usefulness of this SRPE was validated by applying the procedure to extract protein from different Brassica oilseeds and for screening an ethyl methane sulfonate (EMS) mutant population of Brassica rapa R-0-18. The outcomes provide useful methodology for identifying and characterizing the SSPs in the SRPE." @default.
• W3121004677 created "2021-01-18" @default.
• W3121004677 creator A5001388944 @default.
• W3121004677 creator A5020283413 @default.
• W3121004677 creator A5040407767 @default.
• W3121004677 date "2021-01-08" @default.
• W3121004677 modified "2023-09-25" @default.
• W3121004677 title "A Single Seed Protein Extraction Protocol for Characterizing Brassica Seed Storage Proteins" @default.
• W3121004677 cites W1526400750 @default.
• W3121004677 cites W1933943405 @default.
• W3121004677 cites W1967735171 @default.
• W3121004677 cites W1970729492 @default.
• W3121004677 cites W1982655765 @default.
• W3121004677 cites W1986942704 @default.
• W3121004677 cites W1989914042 @default.
• W3121004677 cites W1990028986 @default.
• W3121004677 cites W1990967090 @default.
• W3121004677 cites W1997723318 @default.
• W3121004677 cites W2006275865 @default.
• W3121004677 cites W2007993952 @default.
• W3121004677 cites W2011388096 @default.
• W3121004677 cites W2013578514 @default.
• W3121004677 cites W2024374308 @default.
• W3121004677 cites W2032091344 @default.
• W3121004677 cites W2034740746 @default.
• W3121004677 cites W2034981379 @default.
• W3121004677 cites W2036750363 @default.
• W3121004677 cites W2046900412 @default.
• W3121004677 cites W2049901523 @default.
• W3121004677 cites W2050424135 @default.
• W3121004677 cites W2052681073 @default.
• W3121004677 cites W2052905902 @default.
• W3121004677 cites W2063673586 @default.
• W3121004677 cites W2065547538 @default.
• W3121004677 cites W2072558162 @default.
• W3121004677 cites W2119061815 @default.
• W3121004677 cites W2125882252 @default.
• W3121004677 cites W2131871317 @default.
• W3121004677 cites W2137439541 @default.
• W3121004677 cites W2141889620 @default.
• W3121004677 cites W2142352920 @default.
• W3121004677 cites W2171257497 @default.
• W3121004677 cites W2510127456 @default.
• W3121004677 cites W2539685506 @default.
• W3121004677 cites W2548592960 @default.
• W3121004677 cites W2607339918 @default.
• W3121004677 cites W2793143997 @default.
• W3121004677 cites W2808529713 @default.
• W3121004677 cites W2922397686 @default.
• W3121004677 cites W2950342080 @default.
• W3121004677 cites W2954996093 @default.
• W3121004677 cites W2963897764 @default.
• W3121004677 cites W3014179093 @default.
• W3121004677 cites W3017199751 @default.
• W3121004677 cites W3023877006 @default.
• W3121004677 cites W3048305500 @default.
• W3121004677 cites W3085851600 @default.
• W3121004677 cites W3093661926 @default.
• W3121004677 cites W3097412795 @default.
• W3121004677 cites W4253316678 @default.
• W3121004677 doi "https://doi.org/10.3390/agronomy11010107" @default.
• W3121004677 hasPublicationYear "2021" @default.
• W3121004677 type Work @default.
• W3121004677 sameAs 3121004677 @default.
• W3121004677 citedByCount "6" @default.
• W3121004677 countsByYear W31210046772021 @default.
• W3121004677 countsByYear W31210046772022 @default.
• W3121004677 crossrefType "journal-article" @default.
• W3121004677 hasAuthorship W3121004677A5001388944 @default.
• W3121004677 hasAuthorship W3121004677A5020283413 @default.
• W3121004677 hasAuthorship W3121004677A5040407767 @default.
• W3121004677 hasBestOaLocation W31210046771 @default.
• W3121004677 hasConcept C134121241 @default.
• W3121004677 hasConcept C185592680 @default.
• W3121004677 hasConcept C191897082 @default.
• W3121004677 hasConcept C192562407 @default.
• W3121004677 hasConcept C2776747608 @default.
• W3121004677 hasConcept C2779726014 @default.
• W3121004677 hasConcept C2993030687 @default.
• W3121004677 hasConcept C31903555 @default.
• W3121004677 hasConcept C43617362 @default.
• W3121004677 hasConcept C4725764 @default.
• W3121004677 hasConcept C6557445 @default.
• W3121004677 hasConcept C86803240 @default.
• W3121004677 hasConcept C8985478 @default.
• W3121004677 hasConceptScore W3121004677C134121241 @default.
• W3121004677 hasConceptScore W3121004677C185592680 @default.
• W3121004677 hasConceptScore W3121004677C191897082 @default.
• W3121004677 hasConceptScore W3121004677C192562407 @default.
• W3121004677 hasConceptScore W3121004677C2776747608 @default.
• W3121004677 hasConceptScore W3121004677C2779726014 @default.
• W3121004677 hasConceptScore W3121004677C2993030687 @default.
• W3121004677 hasConceptScore W3121004677C31903555 @default.
• W3121004677 hasConceptScore W3121004677C43617362 @default.
• W3121004677 hasConceptScore W3121004677C4725764 @default.
• W3121004677 hasConceptScore W3121004677C6557445 @default.
• W3121004677 hasConceptScore W3121004677C86803240 @default.
• W3121004677 hasConceptScore W3121004677C8985478 @default.

• next