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- W3121009309 abstract "Author(s): Alvarez, Adrian | Advisor(s): Kummel, Andrew C | Abstract: Protein A chromatography is the industry standard method for the purification of monoclonal antibodies. In this work, an emulsion templated sol-gel reaction is used to synthesize rigid, millimeter-scale SiO2 particles with hierarchical porosity, presenting an alternative to existing protein A chromatography resins for high throughput purification. An average particle diameter of 0.61mm allows for increased packed bed length/width ratios and high mobile phase flow rates to be used with minimal pressure drop. FITC conjugated BSA and fluorescence microscopy are used to show that micron-scale, spherical pores (average diameter = 4.69µm) allow for unhindered diffusion of protein throughout the particles. High temperature calcination (1000°C) is used to induce SiO2 reflow and increase intraparticle wall density (decreasing wall thickness from 1.48µm to 1.04µm, p = 0.014), significantly increasing the overall particle strength. A BCA assay and fluorescence spectroscopy are used to show that treating the particles with concentrated H2O2 increases the immobilized protein ligand density from 6.8 to 24.0 mg protein/mL particles due to an increase in SiO2 surface hydroxyl density. Initial protein separation testing shows poor column efficiency with early protein breakthrough, suggesting mobile phase channeling between particles and a need to sieve the particles to decrease the size distribution." @default.
- W3121009309 created "2021-01-18" @default.
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- W3121009309 date "2018-01-01" @default.
- W3121009309 modified "2023-09-27" @default.
- W3121009309 title "Solid Substrate for High Throughput Protein Chromatography" @default.
- W3121009309 hasPublicationYear "2018" @default.
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