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- W3121033145 abstract "The components and subprocesses underlying the formation of COPI-coated vesicles at the Golgi are well understood. The coating cascade is initiated after the small GTPase Arf1 is activated by the Sec7 domain-containing guanine nucleotide exchange factor GBF1 (Golgi brefeldin A resistant guanine nucleotide exchange factor 1). This causes a conformational shift within Arf1 that facilitates stable association of Arf1 with the membrane, a process required for subsequent recruitment of the COPI coat. Although we have atomic-level knowledge of Arf1 activation by Sec7 domain-containing GEFs, our understanding of the biophysical processes regulating Arf1 and GBF1 dynamics is limited. We used fluorescence recovery after photobleaching data and kinetic Monte Carlo simulation to assess the behavior of Arf1 and GBF1 during COPI vesicle formation in live cells. Our analyses suggest that Arf1 and GBF1 associate with Golgi membranes independently, with an excess of GBF1 relative to Arf1. Furthermore, the GBF1-mediated Arf1 activation is much faster than GBF1 cycling on/off the membrane, suggesting that GBF1 is regulated by processes other than its interactions Arf1. Interestingly, modeling the behavior of the catalytically inactive GBF1/E794K mutant stabilized on the membrane is inconsistent with the formation of a stable complex between it and an endogenous Arf1 and suggests that GBF1/E794K is stabilized on the membrane independently of complex formation." @default.
- W3121033145 created "2021-01-18" @default.
- W3121033145 creator A5008771840 @default.
- W3121033145 creator A5043581086 @default.
- W3121033145 creator A5043834058 @default.
- W3121033145 creator A5050827758 @default.
- W3121033145 creator A5078493599 @default.
- W3121033145 creator A5084650029 @default.
- W3121033145 date "2021-03-01" @default.
- W3121033145 modified "2023-10-16" @default.
- W3121033145 title "Modeling the dynamic behaviors of the COPI vesicle formation regulators, the small GTPase Arf1 and its activating Sec7 guanine nucleotide exchange factor GBF1 on Golgi membranes" @default.
- W3121033145 cites W1497255723 @default.
- W3121033145 cites W1544033945 @default.
- W3121033145 cites W1567984016 @default.
- W3121033145 cites W1589276697 @default.
- W3121033145 cites W1599576642 @default.
- W3121033145 cites W1673824819 @default.
- W3121033145 cites W1969143608 @default.
- W3121033145 cites W1971505763 @default.
- W3121033145 cites W1983657784 @default.
- W3121033145 cites W1990130046 @default.
- W3121033145 cites W1992004763 @default.
- W3121033145 cites W1993290689 @default.
- W3121033145 cites W1995490800 @default.
- W3121033145 cites W2002426370 @default.
- W3121033145 cites W2004863845 @default.
- W3121033145 cites W2005101090 @default.
- W3121033145 cites W2006385755 @default.
- W3121033145 cites W2009209652 @default.
- W3121033145 cites W2009616915 @default.
- W3121033145 cites W2009776121 @default.
- W3121033145 cites W2017934591 @default.
- W3121033145 cites W2022743955 @default.
- W3121033145 cites W2024324386 @default.
- W3121033145 cites W2029354255 @default.
- W3121033145 cites W2029581399 @default.
- W3121033145 cites W2029922958 @default.
- W3121033145 cites W2030902990 @default.
- W3121033145 cites W2032718118 @default.
- W3121033145 cites W2036420384 @default.
- W3121033145 cites W2041073272 @default.
- W3121033145 cites W2045092507 @default.
- W3121033145 cites W2045765657 @default.
- W3121033145 cites W2047261885 @default.
- W3121033145 cites W2050842751 @default.
- W3121033145 cites W2054821964 @default.
- W3121033145 cites W2058210463 @default.
- W3121033145 cites W2064693369 @default.
- W3121033145 cites W2067790068 @default.
- W3121033145 cites W2069980734 @default.
- W3121033145 cites W2070992410 @default.
- W3121033145 cites W2072539493 @default.
- W3121033145 cites W2076758951 @default.
- W3121033145 cites W2081027637 @default.
- W3121033145 cites W2086906681 @default.
- W3121033145 cites W2089191944 @default.
- W3121033145 cites W2090216212 @default.
- W3121033145 cites W2092897825 @default.
- W3121033145 cites W2094152798 @default.
- W3121033145 cites W2097996196 @default.
- W3121033145 cites W2103122002 @default.
- W3121033145 cites W2104360518 @default.
- W3121033145 cites W2116221858 @default.
- W3121033145 cites W2118434776 @default.
- W3121033145 cites W2125366654 @default.
- W3121033145 cites W2133211760 @default.
- W3121033145 cites W2136455040 @default.
- W3121033145 cites W2136767661 @default.
- W3121033145 cites W2137872713 @default.
- W3121033145 cites W2146341607 @default.
- W3121033145 cites W2149743336 @default.
- W3121033145 cites W2150208587 @default.
- W3121033145 cites W2153624331 @default.
- W3121033145 cites W2156816000 @default.
- W3121033145 cites W2161080918 @default.
- W3121033145 cites W2167994809 @default.
- W3121033145 cites W2168740561 @default.
- W3121033145 cites W2170171321 @default.
- W3121033145 cites W2171810148 @default.
- W3121033145 cites W2233644193 @default.
- W3121033145 cites W2260214926 @default.
- W3121033145 cites W2500974730 @default.
- W3121033145 cites W2530851561 @default.
- W3121033145 cites W2753400265 @default.
- W3121033145 cites W2779083329 @default.
- W3121033145 cites W2783941908 @default.
- W3121033145 cites W2793873144 @default.
- W3121033145 cites W2934275657 @default.
- W3121033145 cites W2948449490 @default.
- W3121033145 cites W2967614896 @default.
- W3121033145 cites W2973105130 @default.
- W3121033145 cites W3048948918 @default.
- W3121033145 cites W4212799224 @default.
- W3121033145 doi "https://doi.org/10.1091/mbc.e20-09-0587" @default.
- W3121033145 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8098855" @default.
- W3121033145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33405949" @default.
- W3121033145 hasPublicationYear "2021" @default.
- W3121033145 type Work @default.