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• W3121043980 endingPage "107738" @default.
• W3121043980 startingPage "107738" @default.
• W3121043980 abstract "• An ultrasensing biosensor has been fabricated for food toxin detection. • A metal free nanostructured g-C 3 N 4 used for fabrication of biosensing platform. • A phenothiazine dye has been used for the functionalization of g-C 3 N 4 nanosheets. • Developed biosensor able to detect food toxin with LOD of 0.328 fg mL −1 . We report results of the studies related to the fabrication of thionine functionalized graphitic carbon nitride nanosheets based ultrasensing platform for food toxin (Aflatoxin B1, AfB 1 ) detection. The synthesis of graphitic carbon nitride nanosheets (g-C 3 N 4 ) was carried out by polycondensation of melamine followed by chemical exfoliation. Further, thionine was used for the functionalization of g-C 3 N 4 (Thn/g-C 3 N 4 ) and deposited electrophoretically onto the indium tin oxide (ITO) coated glass electrode. The fabricated Thn/g-C 3 N 4 /ITO electrode was covalently immobilized by EDC-NHS chemistry with anti-aflatoxin B1 (anti-AfB 1 ) followed by blocking of non-specific sites using BSA molecules. For structural, morphological, functional and electrochemical properties analysis of synthesized nanomaterials and fabricated electrodes X-ray diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, atomic force microscopy and cyclic voltammetry techniques were used. The electrochemical response studies of the fabricated biosensing platform (BSA/anti-AfB 1 /Thn/g-C 3 N 4 /ITO) were carried out towards detection of AfB 1 antigen using cyclic voltammetry technique. The obtained electrochemical results indicate that the fabricated biosensing electrode having ability to detect AfB 1 with lower limit of detection of 0.328 fg mL −1 , linear detection range in between 1 fg mL −1 to 1 ng mL −1 , sensitivity of 4.85 μA log [ng −1 mL] cm −2 with stability upto 7 weeks." @default.
• W3121043980 created "2021-01-18" @default.
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• W3121043980 date "2021-06-01" @default.
• W3121043980 modified "2023-10-17" @default.
• W3121043980 title "Nanostructured graphitic carbon nitride based ultrasensing electrochemical biosensor for food toxin detection" @default.
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• W3121043980 doi "https://doi.org/10.1016/j.bioelechem.2021.107738" @default.
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