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- W3121222708 abstract "Enzyme-catalysed asymmetric reduction of ketones is an attractive tool for the production of chiral building blocks or precursors for the synthesis of bioactive compounds. Expression of robust ketoreductase (KRED) from Hansenula polymorpha was upscaled and applied for the asymmetric reduction of 31 prochiral carbonyl compounds (aliphatic and aromatic ketones, diketones and β-keto esters) to the corresponding optically pure hydroxy compounds. Biotransformations were performed with the purified recombinant KRED together with NADP+ recycling glucose dehydrogenase (GDH, Bacillus megaterium), both overexpressed in Escherichia coli BL21(DE3). Maximum activity of KRED for biotransformation of ethyl-2-methylacetoacetate achieved by the high cell density cultivation was 2499.7 ± 234 U g–1DCW and 8.47 ± 0.40 U·mg–1E, respectively. The KRED from Hansenula polymorpha is a very versatile enzyme with broad substrate specificity and high activity towards carbonyl substrates with various structural features. Among the 36 carbonyl substrates screened in this study, the KRED showed activity with 31, with high enantioselectivity in most cases. With several ketones, the Hansenula polymorpha KRED catalysed preferentially the formation of the (R)-secondary alcohols, which is highly valued." @default.
- W3121222708 created "2021-02-01" @default.
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- W3121222708 creator A5078560752 @default.
- W3121222708 date "2021-02-01" @default.
- W3121222708 modified "2023-10-17" @default.
- W3121222708 title "Application of robust ketoreductase from Hansenula polymorpha for the reduction of carbonyl compounds" @default.
- W3121222708 cites W1046814945 @default.
- W3121222708 cites W1671708621 @default.
- W3121222708 cites W1965886551 @default.
- W3121222708 cites W1967354368 @default.
- W3121222708 cites W1968998670 @default.
- W3121222708 cites W1970281466 @default.
- W3121222708 cites W1971392767 @default.
- W3121222708 cites W1972486342 @default.
- W3121222708 cites W1975830479 @default.
- W3121222708 cites W1976840804 @default.
- W3121222708 cites W1977477798 @default.
- W3121222708 cites W1982441403 @default.
- W3121222708 cites W1983600006 @default.
- W3121222708 cites W1984180361 @default.
- W3121222708 cites W1984244651 @default.
- W3121222708 cites W1988456660 @default.
- W3121222708 cites W1988530421 @default.
- W3121222708 cites W1995901307 @default.
- W3121222708 cites W1997084279 @default.
- W3121222708 cites W1999265543 @default.
- W3121222708 cites W2005692012 @default.
- W3121222708 cites W2007065788 @default.
- W3121222708 cites W2010777804 @default.
- W3121222708 cites W2013955651 @default.
- W3121222708 cites W2015936762 @default.
- W3121222708 cites W2016364851 @default.
- W3121222708 cites W2018964331 @default.
- W3121222708 cites W2019051049 @default.
- W3121222708 cites W2019243543 @default.
- W3121222708 cites W2026370090 @default.
- W3121222708 cites W2044863181 @default.
- W3121222708 cites W2045774802 @default.
- W3121222708 cites W2050561063 @default.
- W3121222708 cites W2050890077 @default.
- W3121222708 cites W2053034842 @default.
- W3121222708 cites W2053729654 @default.
- W3121222708 cites W2053914556 @default.
- W3121222708 cites W2055538334 @default.
- W3121222708 cites W2059144438 @default.
- W3121222708 cites W2063089728 @default.
- W3121222708 cites W2064474303 @default.
- W3121222708 cites W2069439613 @default.
- W3121222708 cites W2071891855 @default.
- W3121222708 cites W2086652584 @default.
- W3121222708 cites W2087088755 @default.
- W3121222708 cites W2089752219 @default.
- W3121222708 cites W2090552359 @default.
- W3121222708 cites W2092109679 @default.
- W3121222708 cites W2096271778 @default.
- W3121222708 cites W2097674466 @default.
- W3121222708 cites W2108729127 @default.
- W3121222708 cites W2111769513 @default.
- W3121222708 cites W2115493151 @default.
- W3121222708 cites W2125975586 @default.
- W3121222708 cites W2133502161 @default.
- W3121222708 cites W2164449703 @default.
- W3121222708 cites W2164461570 @default.
- W3121222708 cites W2189631135 @default.
- W3121222708 cites W2316549165 @default.
- W3121222708 cites W2323943045 @default.
- W3121222708 cites W2324692185 @default.
- W3121222708 cites W2397715284 @default.
- W3121222708 cites W2404065769 @default.
- W3121222708 cites W2433499588 @default.
- W3121222708 cites W2618742178 @default.
- W3121222708 cites W2744432936 @default.
- W3121222708 cites W2751743037 @default.
- W3121222708 cites W2755037339 @default.
- W3121222708 cites W2783073377 @default.
- W3121222708 cites W2783629329 @default.
- W3121222708 cites W2785293120 @default.
- W3121222708 cites W2802665863 @default.
- W3121222708 cites W2804869845 @default.
- W3121222708 cites W2931046462 @default.
- W3121222708 cites W2949494005 @default.
- W3121222708 cites W2949995997 @default.
- W3121222708 cites W2950106778 @default.
- W3121222708 cites W2951230724 @default.
- W3121222708 cites W2953021976 @default.
- W3121222708 cites W2975482714 @default.
- W3121222708 cites W4243146789 @default.
- W3121222708 cites W1982906586 @default.
- W3121222708 doi "https://doi.org/10.1016/j.mcat.2020.111364" @default.
- W3121222708 hasPublicationYear "2021" @default.
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