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• W3121781254 abstract "Abstract Measurement and reconstruction of an elemental image of large brain tissue will be beneficial to the diagnosis of neurological brain diseases. Herein, laser‐induced breakdown spectroscopy (LIBS) is introduced for three dimensional (3D) elemental analysis of paraffin‐embedded mouse brain tissue blocks. It is used for the first time towards the mapping of mouse brain block samples. A micro‐LIBS prototype is developed for brain elemental imaging and a layer‐by‐layer approach is used to reconstruct the 3D distribution of Ca, Mg, Na, Cu, and P in the brain tissue. Images are captured with 50 μm lateral resolution and 300 μm depth resolution. The images show that the reclamation area of the cortex surface is enriched with Ca and Mg. In contrast, the Cu distribution is circular and is found primarily in the entirety of the cerebral cortex for the paraffin‐embedded brain samples. Elemental imaging results suggest that the highest P intensity is found in the cerebellum nearby the middle sagittal plane in the left‐brain paraffin block. These preliminary results indicate that LIBS is a potentially powerful tool for elemental bioimaging of the whole brain and may further improve the understanding of complex brain mechanisms." @default.
• W3121781254 created "2021-02-01" @default.
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• W3121781254 date "2021-02-10" @default.
• W3121781254 modified "2023-09-25" @default.
• W3121781254 title "Ex vivo <scp>three‐dimensional</scp> elemental imaging of mouse brain tissue block by laser‐induced breakdown spectroscopy" @default.
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• W3121781254 doi "https://doi.org/10.1002/jbio.202000479" @default.
• W3121781254 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33512064" @default.
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