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• W3121900526 abstract "Increased brain microvascular permeability and disruption of blood-brain barrier (BBB) function are among hallmarks of several acute neurodegenerative disorders, including stroke. Numerous studies suggest the involvement of bradykinin (BK), neurotensin (NT) and substance P (SP) in BBB impairment and oedema formation after stroke; however, there is paucity of data in regard to the direct effects of these peptides on the brain microvascular endothelial cells (BMECs) and BBB. The present study aimed to evaluate the direct effects of BK, NT and SP on the permeability of BBB in an in vitro model based on human induced pluripotent stem cell (iPSC)-derived BMECs. Our data indicate that all three peptides increase BBB permeability in a concentration-dependent manner in an in vitro model formed from two different iPSC lines (CTR90F and CTR65M) and widely used hCMEC/D3 human BMECs. The combination of BK, NT and SP at a sub-effective concentration also resulted in increased BBB permeability in the iPSC-derived model indicating potentiation of their action. Furthermore, we observed abrogation of BK, NT and SP effects with pretreatment of pharmacological blockers targeting their specific receptors. Additional mechanistic studies indicate that the short-term effects of these peptides are not mediated through alteration of tight-junction proteins claudin-5 and occludin, but likely involve redistribution of F-actin and secretion of vascular endothelial growth factor. This is the first experimental study to document the increased permeability of the BBB in response to direct action of NT in an in vitro model. In addition, our study confirms the expected but not well-documented, direct effect of SP on BBB permeability and adds to the well-recognised actions of BK on BBB. Lastly, we demonstrate that peptidase neurolysin can neutralise the effects of these peptides on BBB, suggesting potential therapeutic implications." @default.
• W3121900526 created "2021-02-01" @default.
• W3121900526 creator A5012911330 @default.
• W3121900526 creator A5037761449 @default.
• W3121900526 creator A5057900193 @default.
• W3121900526 date "2021-01-28" @default.
• W3121900526 modified "2023-10-16" @default.
• W3121900526 title "Neurolysin substrates bradykinin, neurotensin and substance P enhance brain microvascular permeability in a human in vitro model" @default.
• W3121900526 cites W127165557 @default.
• W3121900526 cites W1511805726 @default.
• W3121900526 cites W1571217400 @default.
• W3121900526 cites W1577408613 @default.
• W3121900526 cites W1577821076 @default.
• W3121900526 cites W1882745786 @default.
• W3121900526 cites W1964948989 @default.
• W3121900526 cites W1967289199 @default.
• W3121900526 cites W1975857820 @default.
• W3121900526 cites W1993184426 @default.
• W3121900526 cites W1993868631 @default.
• W3121900526 cites W1998026311 @default.
• W3121900526 cites W1998151033 @default.
• W3121900526 cites W1998855830 @default.
• W3121900526 cites W2005675003 @default.
• W3121900526 cites W2007777786 @default.
• W3121900526 cites W2010649750 @default.
• W3121900526 cites W2022396816 @default.
• W3121900526 cites W2023370394 @default.
• W3121900526 cites W2027783302 @default.
• W3121900526 cites W2037261229 @default.
• W3121900526 cites W2039510851 @default.
• W3121900526 cites W2040695574 @default.
• W3121900526 cites W2042883482 @default.
• W3121900526 cites W2046895286 @default.
• W3121900526 cites W2047075745 @default.
• W3121900526 cites W2052712643 @default.
• W3121900526 cites W2056069994 @default.
• W3121900526 cites W2058019780 @default.
• W3121900526 cites W2068201999 @default.
• W3121900526 cites W2069289953 @default.
• W3121900526 cites W2070902035 @default.
• W3121900526 cites W2074766850 @default.
• W3121900526 cites W2079282810 @default.
• W3121900526 cites W2079729010 @default.
• W3121900526 cites W2080557077 @default.
• W3121900526 cites W2081858473 @default.
• W3121900526 cites W2083387643 @default.
• W3121900526 cites W2084498863 @default.
• W3121900526 cites W2085167114 @default.
• W3121900526 cites W2087997311 @default.
• W3121900526 cites W2092103890 @default.
• W3121900526 cites W2094557014 @default.
• W3121900526 cites W2099813941 @default.
• W3121900526 cites W2101494997 @default.
• W3121900526 cites W2105090965 @default.
• W3121900526 cites W2107798346 @default.
• W3121900526 cites W2108798510 @default.
• W3121900526 cites W2109726055 @default.
• W3121900526 cites W2121969098 @default.
• W3121900526 cites W2124632438 @default.
• W3121900526 cites W2127217689 @default.
• W3121900526 cites W2130229686 @default.
• W3121900526 cites W2138974986 @default.
• W3121900526 cites W2141909491 @default.
• W3121900526 cites W2142046859 @default.
• W3121900526 cites W2145191356 @default.
• W3121900526 cites W2145798230 @default.
• W3121900526 cites W2155436051 @default.
• W3121900526 cites W2169362733 @default.
• W3121900526 cites W2263957136 @default.
• W3121900526 cites W2333238186 @default.
• W3121900526 cites W2417704522 @default.
• W3121900526 cites W2474308644 @default.
• W3121900526 cites W2478050710 @default.
• W3121900526 cites W2518946754 @default.
• W3121900526 cites W2523566149 @default.
• W3121900526 cites W2541782532 @default.
• W3121900526 cites W2605762341 @default.
• W3121900526 cites W2606984417 @default.
• W3121900526 cites W2739399469 @default.
• W3121900526 cites W2743362345 @default.
• W3121900526 cites W2749724913 @default.
• W3121900526 cites W2765147576 @default.
• W3121900526 cites W2788259524 @default.
• W3121900526 cites W2791453970 @default.
• W3121900526 cites W2795217332 @default.
• W3121900526 cites W2889244502 @default.
• W3121900526 cites W2922933922 @default.
• W3121900526 cites W2950150743 @default.
• W3121900526 cites W2958422518 @default.
• W3121900526 cites W2971633026 @default.
• W3121900526 cites W2973062996 @default.
• W3121900526 cites W3012508230 @default.
• W3121900526 cites W3048904126 @default.
• W3121900526 cites W90904735 @default.
• W3121900526 doi "https://doi.org/10.1111/jne.12931" @default.
• W3121900526 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8166215" @default.
• W3121900526 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33506602" @default.
• W3121900526 hasPublicationYear "2021" @default.
• W3121900526 type Work @default.
• W3121900526 sameAs 3121900526 @default.

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