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- W3122026721 abstract "Summary While Cre-dependent viral systems permit the manipulation of many neuron types, some cell populations cannot be targeted by a single DNA recombinase. Although the combined use of Flp and Cre recombinases can overcome this limitation, insufficient recombinase activity can reduce the efficacy of existing Cre+Flp-dependent viral systems. We developed a sensitive dual recombinase-activated viral approach: tTA-driven Recombinase-Guided Intersectional Targeting (tTARGIT) AAVs. tTARGIT AAVs utilize a Flp-dependent tetracycline transactivator (tTA) “Driver” AAV and a tetracycline response element (TRE)-driven, Cre-dependent “Payload” AAV to express the transgene of interest. We employed this system in Slc17a6 FlpO ;Lepr Cre mice to manipulate LepRb neurons of the ventromedial hypothalamus (VMH; LepRb VMH neurons) while omitting neighboring LepRb populations. We defined the circuitry of LepRb VMH neurons and roles for these cells in the control of food intake and energy expenditure. Thus, the tTARGIT system mediates robust recombinase-sensitive transgene expression, permitting the precise manipulation of previously intractable neural populations." @default.
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- W3122026721 date "2021-01-27" @default.
- W3122026721 modified "2023-09-27" @default.
- W3122026721 title "tTARGIT AAVs: A sensitive and flexible method to manipulate intersectional neuronal populations" @default.
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- W3122026721 doi "https://doi.org/10.1101/2021.01.26.428259" @default.
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