Matches in SemOpenAlex for { <https://semopenalex.org/work/W31222408> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W31222408 endingPage "1520" @default.
- W31222408 startingPage "1520" @default.
- W31222408 abstract "1520 Objectives Urokinase plasminogen activator (uPA) and its cell surface receptor (uPAR) are over-expressed in breast cancer as well as in several other human cancers and play an important role in tumor growth, metastasis and cancer prognosis. The goal of this project was to develop 99mTc labeled uPAR-targeted radiotracers as potential tumor-targeted imaging probes for non-invasive in vivo imaging of breast cancer. Methods Two small peptides, a C-terminal His6-tagged amino terminal fragment (ATF, ~17kDa), known to be uPAR specific, and an N-terminal NTA-nine-mer peptide (AE105, 1.4 kDa), an uPAR antagonist, were radiolabeled with [99mTc(CO)3(H2O)3]+ produced via an IsoLink kit. 99mTc-ATF was purified by centrifugal ultrafiltration and its 99mTc labeling was confirmed using a 15% SDS-PAGE gel with exposure to radiographic film overnight at 4°C; 99mTc-AE105 was isolated by HPLC. Stability of both radiotracers was determined in PBS buffer pH 7.4 and with histidine challenge; in vitro uptake of 99mTc-ATF in human breast cancer cell lines, MDA-MB-231 that represent a high level of uPAR expression and T47D that has a very low or negative level of uPAR, was also measured. Results Both radiotracers were stable at PBS buffer pH 7.4 for 24 h and no significant re-oxidation, ligand exchange or decomposition were observed even at a 1000-fold molar excess of histidine for 6 h at room temperature. 99mTc-ATF activity was detected at the 17 kDa location, which corresponds to the size of the ATF-peptide. Binding of 99mTc-ATF peptide to the uPAR positive MDA-MB-231 cancer cells was 2.6 fold higher than that binding to uPAR negative T47D cells showing that 99mTc-ATF retains specificity. In-vitro cell binding of 99mTc-AE105 needs to be assessed. Conclusions 99mTc radiolabeling of small uPAR binding peptides provides a potentially versatile route to the development of tumor-targeting probes. Research Support This work was supported by the NIH grant 1P50 CA128301" @default.
- W31222408 created "2016-06-24" @default.
- W31222408 creator A5041917469 @default.
- W31222408 creator A5049471159 @default.
- W31222408 creator A5052087356 @default.
- W31222408 date "2010-05-01" @default.
- W31222408 modified "2023-09-26" @default.
- W31222408 title "uPAR Targeted SPECT imaging probes for detection of breast cancer" @default.
- W31222408 hasPublicationYear "2010" @default.
- W31222408 type Work @default.
- W31222408 sameAs 31222408 @default.
- W31222408 citedByCount "0" @default.
- W31222408 crossrefType "journal-article" @default.
- W31222408 hasAuthorship W31222408A5041917469 @default.
- W31222408 hasAuthorship W31222408A5049471159 @default.
- W31222408 hasAuthorship W31222408A5052087356 @default.
- W31222408 hasConcept C121608353 @default.
- W31222408 hasConcept C12554922 @default.
- W31222408 hasConcept C126322002 @default.
- W31222408 hasConcept C136339569 @default.
- W31222408 hasConcept C150903083 @default.
- W31222408 hasConcept C153911025 @default.
- W31222408 hasConcept C164144092 @default.
- W31222408 hasConcept C170493617 @default.
- W31222408 hasConcept C185592680 @default.
- W31222408 hasConcept C195687474 @default.
- W31222408 hasConcept C202751555 @default.
- W31222408 hasConcept C207001950 @default.
- W31222408 hasConcept C2777807558 @default.
- W31222408 hasConcept C2779013556 @default.
- W31222408 hasConcept C502942594 @default.
- W31222408 hasConcept C530470458 @default.
- W31222408 hasConcept C55493867 @default.
- W31222408 hasConcept C56219504 @default.
- W31222408 hasConcept C71924100 @default.
- W31222408 hasConcept C86803240 @default.
- W31222408 hasConceptScore W31222408C121608353 @default.
- W31222408 hasConceptScore W31222408C12554922 @default.
- W31222408 hasConceptScore W31222408C126322002 @default.
- W31222408 hasConceptScore W31222408C136339569 @default.
- W31222408 hasConceptScore W31222408C150903083 @default.
- W31222408 hasConceptScore W31222408C153911025 @default.
- W31222408 hasConceptScore W31222408C164144092 @default.
- W31222408 hasConceptScore W31222408C170493617 @default.
- W31222408 hasConceptScore W31222408C185592680 @default.
- W31222408 hasConceptScore W31222408C195687474 @default.
- W31222408 hasConceptScore W31222408C202751555 @default.
- W31222408 hasConceptScore W31222408C207001950 @default.
- W31222408 hasConceptScore W31222408C2777807558 @default.
- W31222408 hasConceptScore W31222408C2779013556 @default.
- W31222408 hasConceptScore W31222408C502942594 @default.
- W31222408 hasConceptScore W31222408C530470458 @default.
- W31222408 hasConceptScore W31222408C55493867 @default.
- W31222408 hasConceptScore W31222408C56219504 @default.
- W31222408 hasConceptScore W31222408C71924100 @default.
- W31222408 hasConceptScore W31222408C86803240 @default.
- W31222408 hasLocation W312224081 @default.
- W31222408 hasOpenAccess W31222408 @default.
- W31222408 hasPrimaryLocation W312224081 @default.
- W31222408 hasRelatedWork W1836071781 @default.
- W31222408 hasRelatedWork W2000981295 @default.
- W31222408 hasRelatedWork W2027627414 @default.
- W31222408 hasRelatedWork W2046390205 @default.
- W31222408 hasRelatedWork W2047520474 @default.
- W31222408 hasRelatedWork W2057318313 @default.
- W31222408 hasRelatedWork W2058920486 @default.
- W31222408 hasRelatedWork W2086517904 @default.
- W31222408 hasRelatedWork W2094719325 @default.
- W31222408 hasRelatedWork W2098818525 @default.
- W31222408 hasRelatedWork W2101956211 @default.
- W31222408 hasRelatedWork W2791221205 @default.
- W31222408 hasRelatedWork W2909630497 @default.
- W31222408 hasRelatedWork W2910846208 @default.
- W31222408 hasRelatedWork W2972502103 @default.
- W31222408 hasRelatedWork W2972744299 @default.
- W31222408 hasRelatedWork W3003784457 @default.
- W31222408 hasRelatedWork W3208056280 @default.
- W31222408 hasRelatedWork W576546244 @default.
- W31222408 hasRelatedWork W613237237 @default.
- W31222408 hasVolume "51" @default.
- W31222408 isParatext "false" @default.
- W31222408 isRetracted "false" @default.
- W31222408 magId "31222408" @default.
- W31222408 workType "article" @default.