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- W3122498547 abstract "Phenoloxidase (PO) is a typical metal enzyme, which requires metal ions as prosthetic groups to enable the full exertion of its activity. To study how metal ions affected the activity and structure of PO enzymes, while providing reference materials for in-depth investigations, we examined the effects of different metal ions (Cu2+, Zn2+, Mg2+, Ca2+, and Ba2+) on their activities. Furthermore, Cu2+ and Mg2+ were selected for further investigation through UV spectra, intrinsic fluorescence spectroscopy, AFM, and FTIR. It was revealed that Cu2+ had a more obvious effect on PO compared to Mg2+. The PO could be activated when the concentrations of Cu2+ and Mg2+ were lower than 10−3 and 10−2 mol/L, respectively, and maximum PO activities (182.14% and 141.02%) were observed at 10−4 mol/L concentrations of Cu2+ and Mg2+. When the concentrations of Cu2+ and Mg2+ were higher than 10−2 and 10−1 mol/L, the activities PO were inhibited. The results of the UV–vis and fluorescence spectra revealed that Cu2+ shaped the tertiary structure of PO, whereas the effect of Mg2+ was slight. The AFM results demonstrated that high concentrations of Cu2+ and Mg2+ resulted in PO aggregation. FTIR analysis indicated that the total content of PO α-helices and β-sheets decreased with higher concentrations of Cu2+ and Mg2+." @default.
- W3122498547 created "2021-02-01" @default.
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- W3122498547 date "2021-03-01" @default.
- W3122498547 modified "2023-10-14" @default.
- W3122498547 title "Effects of metal ions on activity and structure of phenoloxidase in Penaeus vannamei" @default.
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- W3122498547 doi "https://doi.org/10.1016/j.ijbiomac.2021.01.112" @default.
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