Matches in SemOpenAlex for { <https://semopenalex.org/work/W3122852401> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W3122852401 abstract "Given that human amniotic membrane is a valuable biological material not readily available for corneal epithelial tissue engineering, gelatin is considered as a potential alternative to construct a cellular microenvironment. This study investigates, for the first time, the influence of cross-linking density of carbodiimide-treated gelatin matrices on the structures and properties of artificial limbal stem cell niches. Our results showed that an increase in the carbodiimide concentration from 1.5 to 15 mM leads to an upward trend in the structural and suture strength of biopolymers. Furthermore, increasing number of cross-linking bridges capable of linking protein molecules together may reduce their crystallinity. For the samples treated with 50 mM of cross-linker (i.e., the presence of excess N-substituted carbodiimide), abundant N-acylurea was detected, which was detrimental to the in vitro and in vivo ocular biocompatibility of gelatin matrices. Surface roughness and stiffness of biopolymer substrates were found to be positively correlated with carbodiimide-induced cross-link formation. Significant increases of integrin β1 expression, metabolic activity, and ABCG2 expression were noted as the cross-linker concentration increased, suggesting that the bulk crystalline structure and surface roughness/stiffness of niche attributed to the number of cross-linking bridges may have profound effects on a variety of limbal epithelial cell behaviors, including adhesion, proliferation, and stemness maintenance. In summary, taking the advantages of carbodiimide cross-linking-mediated development of gelatin matrices, new niches with tunable cross-linking densities can provide a significant boost to maintain the limbal stem cells during ex vivo expansion." @default.
- W3122852401 created "2021-02-01" @default.
- W3122852401 creator A5006317320 @default.
- W3122852401 creator A5055735453 @default.
- W3122852401 creator A5083623112 @default.
- W3122852401 date "2018-09-18" @default.
- W3122852401 modified "2023-09-23" @default.
- W3122852401 title "Effect of Cross-Linking Density on the Structures and Properties of Carbodiimide-Treated Gelatin Matrices as limbal Stem Cell Niches" @default.
- W3122852401 doi "https://doi.org/10.20944/preprints201809.0358.v1" @default.
- W3122852401 hasPublicationYear "2018" @default.
- W3122852401 type Work @default.
- W3122852401 sameAs 3122852401 @default.
- W3122852401 citedByCount "0" @default.
- W3122852401 crossrefType "posted-content" @default.
- W3122852401 hasAuthorship W3122852401A5006317320 @default.
- W3122852401 hasAuthorship W3122852401A5055735453 @default.
- W3122852401 hasAuthorship W3122852401A5083623112 @default.
- W3122852401 hasConcept C12554922 @default.
- W3122852401 hasConcept C136229726 @default.
- W3122852401 hasConcept C185592680 @default.
- W3122852401 hasConcept C192562407 @default.
- W3122852401 hasConcept C202751555 @default.
- W3122852401 hasConcept C2775997276 @default.
- W3122852401 hasConcept C2776363554 @default.
- W3122852401 hasConcept C2778260815 @default.
- W3122852401 hasConcept C2778823387 @default.
- W3122852401 hasConcept C28328180 @default.
- W3122852401 hasConcept C49892992 @default.
- W3122852401 hasConcept C55493867 @default.
- W3122852401 hasConcept C71924100 @default.
- W3122852401 hasConcept C86803240 @default.
- W3122852401 hasConcept C95444343 @default.
- W3122852401 hasConceptScore W3122852401C12554922 @default.
- W3122852401 hasConceptScore W3122852401C136229726 @default.
- W3122852401 hasConceptScore W3122852401C185592680 @default.
- W3122852401 hasConceptScore W3122852401C192562407 @default.
- W3122852401 hasConceptScore W3122852401C202751555 @default.
- W3122852401 hasConceptScore W3122852401C2775997276 @default.
- W3122852401 hasConceptScore W3122852401C2776363554 @default.
- W3122852401 hasConceptScore W3122852401C2778260815 @default.
- W3122852401 hasConceptScore W3122852401C2778823387 @default.
- W3122852401 hasConceptScore W3122852401C28328180 @default.
- W3122852401 hasConceptScore W3122852401C49892992 @default.
- W3122852401 hasConceptScore W3122852401C55493867 @default.
- W3122852401 hasConceptScore W3122852401C71924100 @default.
- W3122852401 hasConceptScore W3122852401C86803240 @default.
- W3122852401 hasConceptScore W3122852401C95444343 @default.
- W3122852401 hasLocation W31228524011 @default.
- W3122852401 hasOpenAccess W3122852401 @default.
- W3122852401 hasPrimaryLocation W31228524011 @default.
- W3122852401 hasRelatedWork W1498640561 @default.
- W3122852401 hasRelatedWork W1542829551 @default.
- W3122852401 hasRelatedWork W1850213035 @default.
- W3122852401 hasRelatedWork W1983716156 @default.
- W3122852401 hasRelatedWork W1990755234 @default.
- W3122852401 hasRelatedWork W1990997493 @default.
- W3122852401 hasRelatedWork W1993437041 @default.
- W3122852401 hasRelatedWork W2020278199 @default.
- W3122852401 hasRelatedWork W2035543126 @default.
- W3122852401 hasRelatedWork W2054215868 @default.
- W3122852401 hasRelatedWork W2056292710 @default.
- W3122852401 hasRelatedWork W2057732629 @default.
- W3122852401 hasRelatedWork W2058070608 @default.
- W3122852401 hasRelatedWork W2097105667 @default.
- W3122852401 hasRelatedWork W2106790616 @default.
- W3122852401 hasRelatedWork W2767363222 @default.
- W3122852401 hasRelatedWork W2891272445 @default.
- W3122852401 hasRelatedWork W2978464873 @default.
- W3122852401 hasRelatedWork W2993901678 @default.
- W3122852401 hasRelatedWork W3125479681 @default.
- W3122852401 isParatext "false" @default.
- W3122852401 isRetracted "false" @default.
- W3122852401 magId "3122852401" @default.
- W3122852401 workType "article" @default.