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• W3123458428 abstract "Although microbial communities of anaerobic bioreactors have been extensively studied using DNA-based tools, there are still several knowledge gaps regarding the microbiology of the process, in particular integration of all generated data is still limited. One understudied core phylum within anaerobic bioreactors is the phylum Chloroflexi, despite being one of the most abundant groups in anaerobic reactors. In order to address the abundance, diversity and phylogeny of this group in full-scale methanogenic reactors globally distributed, a compilation of 16S ribosomal RNA gene sequence data from 62 full-scale methanogenic reactors studied worldwide, fed either with wastewater treatment anaerobic reactors (WTARs) or solid-waste treatment anaerobic reactors (STARs), was performed. One of the barriers to overcome was comparing data generated using different primer sets and different sequencing platforms. The sequence analysis revealed that the average abundance of Chloroflexi in WTARs was higher than in STARs. Four genera belonging to the Anaerolineae class dominated both WTARs and STARs but the core populations were different. According to the phylogenetic analysis, most of the sequences formed clusters with no cultured representatives. The Anaerolineae class was more abundant in reactors with granular biomass than in reactors with disperse biomass supporting the hypothesis that Anaerolineae play an important role in granule formation and structure due to their filamentous morphology. Cross-study comparisons can be fruitfully used to understand the complexity of the anaerobic digestion process. However, more efforts are needed to standardize protocols and report metadata information." @default.
• W3123458428 created "2021-02-01" @default.
• W3123458428 creator A5013814609 @default.
• W3123458428 creator A5020952113 @default.
• W3123458428 creator A5039670201 @default.
• W3123458428 date "2021-01-20" @default.
• W3123458428 modified "2023-09-30" @default.
• W3123458428 title "Database Mining to Unravel the Ecology of the Phylum Chloroflexi in Methanogenic Full Scale Bioreactors" @default.
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• W3123458428 doi "https://doi.org/10.3389/fmicb.2020.603234" @default.
• W3123458428 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7854539" @default.
• W3123458428 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33552017" @default.
• W3123458428 hasPublicationYear "2021" @default.
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