FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3123485971> ?p ?o ?g. }

• W3123485971 endingPage "109082" @default.
• W3123485971 startingPage "109082" @default.
• W3123485971 abstract "Congo Red (CR) has been used for its binding affinity to amyloid fibrils for the better part of a century. Recently, our laboratory has demonstrated its ability to bind to tau protein as well. Here we describe a novel methodology for fast, thorough, whole-brain labeling of amyloid plaques with CR via perfusion. We tested five different variants which altered the volume of CR, the speed of perfusion, and the solution CR was solubilized in to determine the best results. We determined that intra-cardiac perfusion of animals with 0.5 % CR in 100 ml of 50 % ethanol or perfusion with 0.5 of CR in 100 ml of 10 % neutral buffer formalin both perfused at a rate of 30 ml/min for 3.3 min resulted in the clearest CR labeling, with little to no background noise. Both variants were compatible with subsequent immunolabeling procedures for NU-1, as well as Ferritin and GFAP. Compared to traditional CR plaque labeling methodology, this new method allows for quick whole brain CR-labeling. This reduces the amount of time from days to mere minutes. It also reduces potential for variability that would result from staining slides in batches. Thus, CR-perfusion is a rapid, thorough method that can be utilized to rapidly stain amyloid in the rodent brain." @default.
• W3123485971 created "2021-02-01" @default.
• W3123485971 creator A5022660287 @default.
• W3123485971 creator A5034580412 @default.
• W3123485971 creator A5066668776 @default.
• W3123485971 creator A5084524488 @default.
• W3123485971 date "2021-04-01" @default.
• W3123485971 modified "2023-09-26" @default.
• W3123485971 title "In vivo demonstration of Congo Red labeled amyloid plaques via perfusion in the Alzheimer disease rat model" @default.
• W3123485971 cites W1530572082 @default.
• W3123485971 cites W1792313443 @default.
• W3123485971 cites W1993220836 @default.
• W3123485971 cites W2008764310 @default.
• W3123485971 cites W2017457068 @default.
• W3123485971 cites W2022532556 @default.
• W3123485971 cites W2047202120 @default.
• W3123485971 cites W2052742260 @default.
• W3123485971 cites W2064985104 @default.
• W3123485971 cites W2068915736 @default.
• W3123485971 cites W2075366433 @default.
• W3123485971 cites W2078212921 @default.
• W3123485971 cites W2103440561 @default.
• W3123485971 cites W2155882728 @default.
• W3123485971 cites W2159619745 @default.
• W3123485971 cites W2461810846 @default.
• W3123485971 cites W2788894888 @default.
• W3123485971 cites W2801804366 @default.
• W3123485971 cites W2810780277 @default.
• W3123485971 cites W2899520465 @default.
• W3123485971 cites W3080776564 @default.
• W3123485971 cites W83420530 @default.
• W3123485971 cites W84216516 @default.
• W3123485971 doi "https://doi.org/10.1016/j.jneumeth.2021.109082" @default.
• W3123485971 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33508413" @default.
• W3123485971 hasPublicationYear "2021" @default.
• W3123485971 type Work @default.
• W3123485971 sameAs 3123485971 @default.
• W3123485971 citedByCount "6" @default.
• W3123485971 countsByYear W31234859712022 @default.
• W3123485971 countsByYear W31234859712023 @default.
• W3123485971 crossrefType "journal-article" @default.
• W3123485971 hasAuthorship W3123485971A5022660287 @default.
• W3123485971 hasAuthorship W3123485971A5034580412 @default.
• W3123485971 hasAuthorship W3123485971A5066668776 @default.
• W3123485971 hasAuthorship W3123485971A5084524488 @default.
• W3123485971 hasConcept C135691158 @default.
• W3123485971 hasConcept C142724271 @default.
• W3123485971 hasConcept C146957229 @default.
• W3123485971 hasConcept C150394285 @default.
• W3123485971 hasConcept C150903083 @default.
• W3123485971 hasConcept C153911025 @default.
• W3123485971 hasConcept C164705383 @default.
• W3123485971 hasConcept C178790620 @default.
• W3123485971 hasConcept C185592680 @default.
• W3123485971 hasConcept C204232928 @default.
• W3123485971 hasConcept C207001950 @default.
• W3123485971 hasConcept C2777633098 @default.
• W3123485971 hasConcept C2777790613 @default.
• W3123485971 hasConcept C2777877921 @default.
• W3123485971 hasConcept C2779134260 @default.
• W3123485971 hasConcept C2779155187 @default.
• W3123485971 hasConcept C2779483572 @default.
• W3123485971 hasConcept C2989005 @default.
• W3123485971 hasConcept C40209533 @default.
• W3123485971 hasConcept C55493867 @default.
• W3123485971 hasConcept C71924100 @default.
• W3123485971 hasConcept C86803240 @default.
• W3123485971 hasConceptScore W3123485971C135691158 @default.
• W3123485971 hasConceptScore W3123485971C142724271 @default.
• W3123485971 hasConceptScore W3123485971C146957229 @default.
• W3123485971 hasConceptScore W3123485971C150394285 @default.
• W3123485971 hasConceptScore W3123485971C150903083 @default.
• W3123485971 hasConceptScore W3123485971C153911025 @default.
• W3123485971 hasConceptScore W3123485971C164705383 @default.
• W3123485971 hasConceptScore W3123485971C178790620 @default.
• W3123485971 hasConceptScore W3123485971C185592680 @default.
• W3123485971 hasConceptScore W3123485971C204232928 @default.
• W3123485971 hasConceptScore W3123485971C207001950 @default.
• W3123485971 hasConceptScore W3123485971C2777633098 @default.
• W3123485971 hasConceptScore W3123485971C2777790613 @default.
• W3123485971 hasConceptScore W3123485971C2777877921 @default.
• W3123485971 hasConceptScore W3123485971C2779134260 @default.
• W3123485971 hasConceptScore W3123485971C2779155187 @default.
• W3123485971 hasConceptScore W3123485971C2779483572 @default.
• W3123485971 hasConceptScore W3123485971C2989005 @default.
• W3123485971 hasConceptScore W3123485971C40209533 @default.
• W3123485971 hasConceptScore W3123485971C55493867 @default.
• W3123485971 hasConceptScore W3123485971C71924100 @default.
• W3123485971 hasConceptScore W3123485971C86803240 @default.
• W3123485971 hasLocation W31234859711 @default.
• W3123485971 hasOpenAccess W3123485971 @default.
• W3123485971 hasPrimaryLocation W31234859711 @default.
• W3123485971 hasRelatedWork W1504170807 @default.
• W3123485971 hasRelatedWork W1970927272 @default.
• W3123485971 hasRelatedWork W2013775559 @default.
• W3123485971 hasRelatedWork W2030100345 @default.
• W3123485971 hasRelatedWork W2054390643 @default.
• W3123485971 hasRelatedWork W2094899106 @default.

• next