FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3123824074> ?p ?o ?g. }

• W3123824074 abstract "Abstract Aims Atrial Fibrillation (AF) is an arrhythmia of increasing prevalence in the aging population of developed countries. One of the important indicators of AF is sustained atrial dilatation, highlighting the importance of mechanical overload in the pathophysiology of AF. The mechanisms by which atrial cells, including fibroblasts, sense and react to changing mechanical forces, are not fully elucidated. Here, we characterise stretch-activated ion channels (SAC) in human atrial fibroblasts and changes in SAC-presence and -activity associated with AF. Methods and Results Using primary cultures of human atrial fibroblasts, isolated from patients in sinus rhythm or sustained AF, we combine electrophysiological, molecular and pharmacological tools to identify SAC. Two electrophysiological SAC-signatures were detected, indicative of cation-nonselective and potassium-selective channels. Using siRNA-mediated knockdown, we identified the nonselective SAC as Piezo1. Biophysical properties of the potassium-selective channel, its sensitivity to calcium, paxilline and iberiotoxin (blockers), and NS11021 (activator), indicated presence of calcium-dependent ‘big potassium channels’, BK Ca . In cells from AF patients, Piezo1 activity and mRNA expression levels were higher than in cells from sinus rhythm patients, while BK Ca activity (but not expression) was downregulated. Both Piezo1-knockdown and removal of extracellular calcium from the patch pipette resulted in a significant reduction of BK Ca current during stretch. No co-immunoprecipitation of Piezo1 and BK Ca was detected. Conclusions Human atrial fibroblasts contain at least two types of ion channels that are activated during stretch: Piezo1 and BK Ca . While Piezo1 is directly stretch-activated, the increase in BK Ca activity during mechanical stimulation appears to be mainly secondary to calcium influx via SAC such as Piezo1. During sustained AF, Piezo1 is increased, while BK Ca activity is reduced, highlighting differential regulation of both channels. Our data support the presence and interplay of Piezo1 and BK Ca in human atrial fibroblasts in the absence of physical interactions between the two channel proteins." @default.
• W3123824074 created "2021-02-01" @default.
• W3123824074 creator A5003159972 @default.
• W3123824074 creator A5003450108 @default.
• W3123824074 creator A5006111606 @default.
• W3123824074 creator A5008331250 @default.
• W3123824074 creator A5037563041 @default.
• W3123824074 creator A5039867707 @default.
• W3123824074 creator A5046265150 @default.
• W3123824074 creator A5050747053 @default.
• W3123824074 creator A5053305595 @default.
• W3123824074 creator A5058089749 @default.
• W3123824074 creator A5073141203 @default.
• W3123824074 creator A5077327102 @default.
• W3123824074 creator A5078313605 @default.
• W3123824074 creator A5080288991 @default.
• W3123824074 creator A5084551618 @default.
• W3123824074 creator A5085742591 @default.
• W3123824074 creator A5088073850 @default.
• W3123824074 creator A5090509487 @default.
• W3123824074 creator A5090543746 @default.
• W3123824074 creator A5073174706 @default.
• W3123824074 date "2021-01-21" @default.
• W3123824074 modified "2023-09-25" @default.
• W3123824074 title "Piezo1 and BK_Ca channels in human atrial fibroblasts: interplay and remodelling in atrial fibrillation" @default.
• W3123824074 cites W1499222776 @default.
• W3123824074 cites W1511808429 @default.
• W3123824074 cites W1555584653 @default.
• W3123824074 cites W1972071814 @default.
• W3123824074 cites W1975692469 @default.
• W3123824074 cites W1975767206 @default.
• W3123824074 cites W1976176467 @default.
• W3123824074 cites W1980069683 @default.
• W3123824074 cites W1980913064 @default.
• W3123824074 cites W1984602413 @default.
• W3123824074 cites W1985735643 @default.
• W3123824074 cites W1997829636 @default.
• W3123824074 cites W1999044719 @default.
• W3123824074 cites W1999720993 @default.
• W3123824074 cites W2000598241 @default.
• W3123824074 cites W2014170268 @default.
• W3123824074 cites W2017179259 @default.
• W3123824074 cites W2018761453 @default.
• W3123824074 cites W2020087937 @default.
• W3123824074 cites W2033861216 @default.
• W3123824074 cites W2039208113 @default.
• W3123824074 cites W2050575405 @default.
• W3123824074 cites W2051788770 @default.
• W3123824074 cites W2054974427 @default.
• W3123824074 cites W2067026685 @default.
• W3123824074 cites W2077123850 @default.
• W3123824074 cites W2077446661 @default.
• W3123824074 cites W2078671195 @default.
• W3123824074 cites W2079453878 @default.
• W3123824074 cites W2079689027 @default.
• W3123824074 cites W2081629832 @default.
• W3123824074 cites W2085179557 @default.
• W3123824074 cites W2091983119 @default.
• W3123824074 cites W2104989250 @default.
• W3123824074 cites W2105652374 @default.
• W3123824074 cites W2109713300 @default.
• W3123824074 cites W2118122875 @default.
• W3123824074 cites W2142466990 @default.
• W3123824074 cites W2166599924 @default.
• W3123824074 cites W2168924687 @default.
• W3123824074 cites W2259283850 @default.
• W3123824074 cites W2267163926 @default.
• W3123824074 cites W2506156156 @default.
• W3123824074 cites W2509795914 @default.
• W3123824074 cites W2560738143 @default.
• W3123824074 cites W2609696371 @default.
• W3123824074 cites W2610112373 @default.
• W3123824074 cites W2645647341 @default.
• W3123824074 cites W2736717809 @default.
• W3123824074 cites W2753676541 @default.
• W3123824074 cites W2789232676 @default.
• W3123824074 cites W2888643236 @default.
• W3123824074 cites W2906162080 @default.
• W3123824074 cites W2927703930 @default.
• W3123824074 cites W2949873958 @default.
• W3123824074 cites W2973598194 @default.
• W3123824074 cites W2978100335 @default.
• W3123824074 cites W3021486524 @default.
• W3123824074 cites W3027595869 @default.
• W3123824074 cites W4245452283 @default.
• W3123824074 cites W4252528817 @default.
• W3123824074 doi "https://doi.org/10.1101/2021.01.21.427388" @default.
• W3123824074 hasPublicationYear "2021" @default.
• W3123824074 type Work @default.
• W3123824074 sameAs 3123824074 @default.
• W3123824074 citedByCount "2" @default.
• W3123824074 countsByYear W31238240742021 @default.
• W3123824074 countsByYear W31238240742023 @default.
• W3123824074 crossrefType "posted-content" @default.
• W3123824074 hasAuthorship W3123824074A5003159972 @default.
• W3123824074 hasAuthorship W3123824074A5003450108 @default.
• W3123824074 hasAuthorship W3123824074A5006111606 @default.
• W3123824074 hasAuthorship W3123824074A5008331250 @default.
• W3123824074 hasAuthorship W3123824074A5037563041 @default.
• W3123824074 hasAuthorship W3123824074A5039867707 @default.

• next