FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3124010147> ?p ?o ?g. }

• W3124010147 endingPage "630" @default.
• W3124010147 startingPage "618" @default.
• W3124010147 abstract "Peptides that self-assemble into nanometer-sized pores in lipid bilayers could have utility in a variety of biotechnological and clinical applications if we can understand their physical chemical properties and learn to control their membrane selectivity. To empower such control, we have used synthetic molecular evolution to identify the pH-dependent delivery peptides, a family of peptides that assemble into macromolecule-sized pores in membranes at low peptide concentration but only at pH < ∼6. Further advancements will also require better selectivity for specific membranes. Here, we determine the effect of anionic headgroups and bilayer thickness on the mechanism of action of the pH-dependent delivery peptides by measuring binding, secondary structure, and macromolecular poration. The peptide pHD15 partitions and folds equally well into zwitterionic and anionic membranes but is less potent at pore formation in phosphatidylserine-containing membranes. The peptide also binds and folds similarly in membranes of various thicknesses, but its ability to release macromolecules changes dramatically. It causes potent macromolecular poration in vesicles made from phosphatidylcholine with 14 carbon acyl chains, but macromolecular poration decreases sharply with increasing bilayer thickness and does not occur at any peptide concentration in fluid bilayers made from phosphatidylcholine lipids with 20-carbon acyl chains. The effects of headgroup and bilayer thickness on macromolecular poration cannot be accounted for by the amount of peptide bound but instead reflect an inherent selectivity of the peptide for inserting into the membrane-spanning pore state. Molecular dynamics simulations suggest that the effect of thickness is due to hydrophobic match/mismatch between the membrane-spanning peptide and the bilayer hydrocarbon. This remarkable degree of selectivity based on headgroup and especially bilayer thickness is unusual and suggests ways that pore-forming peptides with exquisite selectivity for specific membranes can be designed or evolved." @default.
• W3124010147 created "2021-02-01" @default.
• W3124010147 creator A5000748364 @default.
• W3124010147 creator A5043039841 @default.
• W3124010147 creator A5058529606 @default.
• W3124010147 creator A5062685696 @default.
• W3124010147 date "2021-02-01" @default.
• W3124010147 modified "2023-10-11" @default.
• W3124010147 title "pH-triggered pore-forming peptides with strong composition-dependent membrane selectivity" @default.
• W3124010147 cites W1501032637 @default.
• W3124010147 cites W1803102843 @default.
• W3124010147 cites W1963680948 @default.
• W3124010147 cites W1965571271 @default.
• W3124010147 cites W1967737979 @default.
• W3124010147 cites W1976499671 @default.
• W3124010147 cites W1978029175 @default.
• W3124010147 cites W1987966996 @default.
• W3124010147 cites W1990550092 @default.
• W3124010147 cites W1994446262 @default.
• W3124010147 cites W1997332996 @default.
• W3124010147 cites W2005386550 @default.
• W3124010147 cites W2006177920 @default.
• W3124010147 cites W2007836674 @default.
• W3124010147 cites W2016572177 @default.
• W3124010147 cites W2017835622 @default.
• W3124010147 cites W2023797283 @default.
• W3124010147 cites W2024156929 @default.
• W3124010147 cites W2024313783 @default.
• W3124010147 cites W2027408247 @default.
• W3124010147 cites W2029667189 @default.
• W3124010147 cites W2031151497 @default.
• W3124010147 cites W2035687084 @default.
• W3124010147 cites W2038813263 @default.
• W3124010147 cites W2038980320 @default.
• W3124010147 cites W2050333027 @default.
• W3124010147 cites W2053444505 @default.
• W3124010147 cites W2054342311 @default.
• W3124010147 cites W205521029 @default.
• W3124010147 cites W2055592362 @default.
• W3124010147 cites W2060259273 @default.
• W3124010147 cites W2062005486 @default.
• W3124010147 cites W2064697628 @default.
• W3124010147 cites W2066414494 @default.
• W3124010147 cites W2067174909 @default.
• W3124010147 cites W2069113791 @default.
• W3124010147 cites W2070839892 @default.
• W3124010147 cites W2071571387 @default.
• W3124010147 cites W2072258007 @default.
• W3124010147 cites W2077178410 @default.
• W3124010147 cites W2079256756 @default.
• W3124010147 cites W2080127910 @default.
• W3124010147 cites W2082148481 @default.
• W3124010147 cites W2086752617 @default.
• W3124010147 cites W2095407378 @default.
• W3124010147 cites W2097212981 @default.
• W3124010147 cites W2101934167 @default.
• W3124010147 cites W2103815459 @default.
• W3124010147 cites W2125872855 @default.
• W3124010147 cites W2126118567 @default.
• W3124010147 cites W2129182145 @default.
• W3124010147 cites W2145137650 @default.
• W3124010147 cites W2150981663 @default.
• W3124010147 cites W2155147384 @default.
• W3124010147 cites W2159861471 @default.
• W3124010147 cites W2162166182 @default.
• W3124010147 cites W2163258998 @default.
• W3124010147 cites W2166311543 @default.
• W3124010147 cites W2171115266 @default.
• W3124010147 cites W2443086882 @default.
• W3124010147 cites W2507698120 @default.
• W3124010147 cites W2561577373 @default.
• W3124010147 cites W2569916494 @default.
• W3124010147 cites W2618923743 @default.
• W3124010147 cites W2762457028 @default.
• W3124010147 cites W2802446650 @default.
• W3124010147 cites W2802616245 @default.
• W3124010147 cites W2910766400 @default.
• W3124010147 cites W2923275068 @default.
• W3124010147 cites W2984065601 @default.
• W3124010147 cites W3023677370 @default.
• W3124010147 doi "https://doi.org/10.1016/j.bpj.2021.01.010" @default.
• W3124010147 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7896028" @default.
• W3124010147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33460594" @default.
• W3124010147 hasPublicationYear "2021" @default.
• W3124010147 type Work @default.
• W3124010147 sameAs 3124010147 @default.
• W3124010147 citedByCount "10" @default.
• W3124010147 countsByYear W31240101472021 @default.
• W3124010147 countsByYear W31240101472022 @default.
• W3124010147 countsByYear W31240101472023 @default.
• W3124010147 crossrefType "journal-article" @default.
• W3124010147 hasAuthorship W3124010147A5000748364 @default.
• W3124010147 hasAuthorship W3124010147A5043039841 @default.
• W3124010147 hasAuthorship W3124010147A5058529606 @default.
• W3124010147 hasAuthorship W3124010147A5062685696 @default.
• W3124010147 hasBestOaLocation W31240101471 @default.
• W3124010147 hasConcept C118792377 @default.
• W3124010147 hasConcept C125501631 @default.

• next