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- W3124013878 abstract "Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA. Here, we provide detailed protocols for expression and purification of the protein from its native host and a cOA degradation assay with the purified enzyme. The methodology should be of interest for researchers studying Sulfolobus, membrane-associated proteins, or type III CRISPR-cas systems. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020)." @default.
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- W3124013878 date "2021-03-01" @default.
- W3124013878 modified "2023-10-03" @default.
- W3124013878 title "Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus" @default.
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- W3124013878 doi "https://doi.org/10.1016/j.xpro.2021.100299" @default.
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