FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3124806557> ?p ?o ?g. }

• W3124806557 endingPage "571" @default.
• W3124806557 startingPage "559" @default.
• W3124806557 abstract "Transduction of primary human natural killer (NK) cells with lentiviral vectors has historically been challenging. We sought to evaluate multiple parameters to optimize lentiviral transduction of human peripheral blood NK cells being expanded to large numbers using a good manufacturing practice (GMP)-compliant protocol that utilizes irradiated lymphoblastoid (LCL) feeder cells. Although prestimulation of NK cells with interleukin (IL)-2 for 2 or more days facilitated transduction with vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped lentivirus, there was a subsequent impairment in the capacity of transduced NK cells to proliferate when stimulated with LCL feeder cells. In contrast, incubation of human NK cells with LCL feeder cells plus IL-2 before transduction in the presence of the TBK1 inhibitor BX795 resulted in efficient lentiviral integration (mean of 23% transgene+ NK cells) and successful subsequent proliferation of the transduced cells. Investigation of multiple internal promoter sequences within the same lentiviral vector revealed differences in percentage and level of transgene expression per NK cell. Bicistronic lentiviral vectors encoding both GFP and proteins suitable for the isolation of transduced cells with magnetic beads led to efficient transgene expression in NK cells. The optimized approaches described herein provide a template for protocols that generate large numbers of fully functional and highly purified lentivirus-transduced NK cells for clinical trials. Transduction of primary human natural killer (NK) cells with lentiviral vectors has historically been challenging. We sought to evaluate multiple parameters to optimize lentiviral transduction of human peripheral blood NK cells being expanded to large numbers using a good manufacturing practice (GMP)-compliant protocol that utilizes irradiated lymphoblastoid (LCL) feeder cells. Although prestimulation of NK cells with interleukin (IL)-2 for 2 or more days facilitated transduction with vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped lentivirus, there was a subsequent impairment in the capacity of transduced NK cells to proliferate when stimulated with LCL feeder cells. In contrast, incubation of human NK cells with LCL feeder cells plus IL-2 before transduction in the presence of the TBK1 inhibitor BX795 resulted in efficient lentiviral integration (mean of 23% transgene+ NK cells) and successful subsequent proliferation of the transduced cells. Investigation of multiple internal promoter sequences within the same lentiviral vector revealed differences in percentage and level of transgene expression per NK cell. Bicistronic lentiviral vectors encoding both GFP and proteins suitable for the isolation of transduced cells with magnetic beads led to efficient transgene expression in NK cells. The optimized approaches described herein provide a template for protocols that generate large numbers of fully functional and highly purified lentivirus-transduced NK cells for clinical trials." @default.
• W3124806557 created "2021-02-01" @default.
• W3124806557 creator A5027573322 @default.
• W3124806557 creator A5028888396 @default.
• W3124806557 creator A5044940530 @default.
• W3124806557 creator A5071903757 @default.
• W3124806557 creator A5073204184 @default.
• W3124806557 creator A5076074380 @default.
• W3124806557 creator A5087705062 @default.
• W3124806557 date "2021-03-01" @default.
• W3124806557 modified "2023-10-15" @default.
• W3124806557 title "Systematic improvements in lentiviral transduction of primary human natural killer cells undergoing ex vivo expansion" @default.
• W3124806557 cites W1504179404 @default.
• W3124806557 cites W1599718730 @default.
• W3124806557 cites W1965962344 @default.
• W3124806557 cites W1980923315 @default.
• W3124806557 cites W1981137720 @default.
• W3124806557 cites W1982206175 @default.
• W3124806557 cites W2003934533 @default.
• W3124806557 cites W2007400263 @default.
• W3124806557 cites W2016510876 @default.
• W3124806557 cites W2018325561 @default.
• W3124806557 cites W2018570885 @default.
• W3124806557 cites W2019995256 @default.
• W3124806557 cites W2020646453 @default.
• W3124806557 cites W2029458815 @default.
• W3124806557 cites W2044360617 @default.
• W3124806557 cites W2054405162 @default.
• W3124806557 cites W2069190585 @default.
• W3124806557 cites W2070120246 @default.
• W3124806557 cites W2091675007 @default.
• W3124806557 cites W2098799914 @default.
• W3124806557 cites W2143693981 @default.
• W3124806557 cites W2165813814 @default.
• W3124806557 cites W2166600445 @default.
• W3124806557 cites W2169619257 @default.
• W3124806557 cites W2319353123 @default.
• W3124806557 cites W2334879529 @default.
• W3124806557 cites W2409620818 @default.
• W3124806557 cites W2419127460 @default.
• W3124806557 cites W2520632223 @default.
• W3124806557 cites W2521461373 @default.
• W3124806557 cites W2738977754 @default.
• W3124806557 cites W2744273874 @default.
• W3124806557 cites W2750168879 @default.
• W3124806557 cites W2754677091 @default.
• W3124806557 cites W2790461174 @default.
• W3124806557 cites W2899003114 @default.
• W3124806557 cites W2932181568 @default.
• W3124806557 cites W2968221494 @default.
• W3124806557 cites W2996286256 @default.
• W3124806557 cites W3004611567 @default.
• W3124806557 doi "https://doi.org/10.1016/j.omtm.2021.01.008" @default.
• W3124806557 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7890427" @default.
• W3124806557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33665226" @default.
• W3124806557 hasPublicationYear "2021" @default.
• W3124806557 type Work @default.
• W3124806557 sameAs 3124806557 @default.
• W3124806557 citedByCount "16" @default.
• W3124806557 countsByYear W31248065572021 @default.
• W3124806557 countsByYear W31248065572022 @default.
• W3124806557 countsByYear W31248065572023 @default.
• W3124806557 crossrefType "journal-article" @default.
• W3124806557 hasAuthorship W3124806557A5027573322 @default.
• W3124806557 hasAuthorship W3124806557A5028888396 @default.
• W3124806557 hasAuthorship W3124806557A5044940530 @default.
• W3124806557 hasAuthorship W3124806557A5071903757 @default.
• W3124806557 hasAuthorship W3124806557A5073204184 @default.
• W3124806557 hasAuthorship W3124806557A5076074380 @default.
• W3124806557 hasAuthorship W3124806557A5087705062 @default.
• W3124806557 hasBestOaLocation W31248065571 @default.
• W3124806557 hasConcept C102230213 @default.
• W3124806557 hasConcept C104317684 @default.
• W3124806557 hasConcept C111599444 @default.
• W3124806557 hasConcept C129374314 @default.
• W3124806557 hasConcept C15152581 @default.
• W3124806557 hasConcept C153911025 @default.
• W3124806557 hasConcept C159047783 @default.
• W3124806557 hasConcept C183315844 @default.
• W3124806557 hasConcept C203014093 @default.
• W3124806557 hasConcept C2522874641 @default.
• W3124806557 hasConcept C2776090121 @default.
• W3124806557 hasConcept C2779057330 @default.
• W3124806557 hasConcept C32470452 @default.
• W3124806557 hasConcept C40767141 @default.
• W3124806557 hasConcept C54355233 @default.
• W3124806557 hasConcept C55493867 @default.
• W3124806557 hasConcept C86803240 @default.
• W3124806557 hasConcept C8891405 @default.
• W3124806557 hasConcept C95444343 @default.
• W3124806557 hasConceptScore W3124806557C102230213 @default.
• W3124806557 hasConceptScore W3124806557C104317684 @default.
• W3124806557 hasConceptScore W3124806557C111599444 @default.
• W3124806557 hasConceptScore W3124806557C129374314 @default.
• W3124806557 hasConceptScore W3124806557C15152581 @default.
• W3124806557 hasConceptScore W3124806557C153911025 @default.
• W3124806557 hasConceptScore W3124806557C159047783 @default.
• W3124806557 hasConceptScore W3124806557C183315844 @default.

• next