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• W3125479914 abstract "Orexin-A (OXA) is a neuropeptide with neuroprotective effect by reducing cerebral ischemia/reperfusion injury (CIRI). Inflammation and apoptosis mediated by astrocyte activation are the key pathological mechanisms for CIRI. We thus attempted to confirm neuroprotective effects of OXA on astrocytic inflammation and apoptosis in CIRI and clarify the relative mechanisms. A middle cerebral artery occlusion and reperfusion (MCAO/R) rat model and U251 glioma cells model subjected to oxygen glucose deprivation and reperfusion (OGD/R) were established, with or without OXA treatment. Neurological deficit score was determined, and cerebral infarct volume was evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Western Blot was used to detect the expressions of NF-κB p65, p-p65, p-ERK, p-p38, GFAP, OX1R, IL-1β, TNF-α, IL-6, iNOS, Bcl-2, Bax, CytC, cleaved caspase-9 and cleaved caspase-3 in vivo and in vitro. Pro-inflammatory cytokines in cell supernatant IL-1β, TNF-α and IL-6 were determined by ELISA. Hoechst 33342 staining was used to detect the apoptosis of astrocyte. Immunofluorescent staining was performed to assess the nuclear translocation of p65 and the expression of GFAP. The results showed that OXA significantly improved neurological deficit score and decreased the volume of infarct area in brain. OXA decreased inflammatory mediators, inhibited astrocyte activation and nuclear translocation of NF-κB and phosphorylation of NF-κB, MAPK/ERK and MAPK/p38. Besides, OXA suppressed apoptosis via upregulating the ratio of Bcl-2/Bax and downregulating cytochrome C, cleaved-caspase-9 and cleaved caspase-3. Overall, it was concluded that OXA exerts neuroprotective effect during CIRI through attenuating astrocytes apoptosis, astrocytes activation and pro-inflammatory cytokines production, by Inhibiting OX1R-mediated NF-κB, MAPK/ERK and MAPK/p38 signaling pathways. The progress in our study is helpful to elucidate the molecular mechanisms of OXA neuroprotection, which could lead to the development of new treatment strategies for ischemic stroke." @default.
• W3125479914 created "2021-02-01" @default.
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• W3125479914 date "2021-11-01" @default.
• W3125479914 modified "2023-10-16" @default.
• W3125479914 title "Orexin-A alleviates astrocytic apoptosis and inflammation via inhibiting OX1R-mediated NF-κB and MAPK signaling pathways in cerebral ischemia/reperfusion injury" @default.
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• W3125479914 cites W1911185606 @default.
• W3125479914 cites W1974980101 @default.
• W3125479914 cites W1979136504 @default.
• W3125479914 cites W1981196417 @default.
• W3125479914 cites W1983061009 @default.
• W3125479914 cites W1991495749 @default.
• W3125479914 cites W1994265630 @default.
• W3125479914 cites W1998106951 @default.
• W3125479914 cites W2001190649 @default.
• W3125479914 cites W2016539734 @default.
• W3125479914 cites W2017281018 @default.
• W3125479914 cites W2028877394 @default.
• W3125479914 cites W2030300220 @default.
• W3125479914 cites W2031044435 @default.
• W3125479914 cites W2037416030 @default.
• W3125479914 cites W2042938039 @default.
• W3125479914 cites W2046149152 @default.
• W3125479914 cites W2049279072 @default.
• W3125479914 cites W2051900634 @default.
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• W3125479914 cites W2076034559 @default.
• W3125479914 cites W2077523355 @default.
• W3125479914 cites W2078033941 @default.
• W3125479914 cites W2081528824 @default.
• W3125479914 cites W2084964323 @default.
• W3125479914 cites W2096978860 @default.
• W3125479914 cites W2101220293 @default.
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• W3125479914 cites W2130198578 @default.
• W3125479914 cites W2131027591 @default.
• W3125479914 cites W2132500820 @default.
• W3125479914 cites W2147357406 @default.
• W3125479914 cites W2155277431 @default.
• W3125479914 cites W2166868001 @default.
• W3125479914 cites W2170806182 @default.
• W3125479914 cites W2208088507 @default.
• W3125479914 cites W2269096641 @default.
• W3125479914 cites W2292727275 @default.
• W3125479914 cites W2398331890 @default.
• W3125479914 cites W2410678570 @default.
• W3125479914 cites W2412690164 @default.
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• W3125479914 cites W2565052042 @default.
• W3125479914 cites W2617157697 @default.
• W3125479914 cites W2766011156 @default.
• W3125479914 cites W2769341285 @default.
• W3125479914 cites W2772954826 @default.
• W3125479914 cites W2898474328 @default.
• W3125479914 cites W2900430109 @default.
• W3125479914 cites W2912343713 @default.
• W3125479914 cites W2949283174 @default.
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• W3125479914 doi "https://doi.org/10.1016/j.bbadis.2021.166230" @default.
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• W3125479914 hasPublicationYear "2021" @default.
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