FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3125712789> ?p ?o ?g. }

• W3125712789 endingPage "394" @default.
• W3125712789 startingPage "394" @default.
• W3125712789 abstract "Background: Aspergillus fumigatus causes life-threatening iinvasive aspergillosis, especially in immunocompromised hosts, and resistance of this filamentous fungus to commonly used antifungal agents has been on the rise. Allicin (diallyl-dithiosulfinate), an allyl-sulfur compound from garlic has been proven to exhibit antimicrobial properties. However, there have been few studies on the antifungal mechanism of allicin against A. fumigatus. Based on our previous studies on the antifungal mechanism of allicin against Candida albicans, an opportunistic yeast pathogen, we hypothesize that allicin would similarly target cell-wall biosynthesis and integrity in A. fumigatus. This study evaluated the expression of cell-wall related genes in A. fumigatus ATCC 36607 upon treatment with allicin. Methods and materials: The minimum inhibitory concentration (MIC) of allicin against A. fumigatus ATCC 36607was determined according to the CLSI M38-A2 guidelines. A. fumigatus was incubated in the presence of allicin at subinhibitory concentrations of 1/4X MIC, 1/2X MIC, and 1X MIC for 48 hours. The cells were harvested post-incubation with allicin and RNA was extracted and reverse-transcribed into cDNA for evaluation of expression of selected genes via real time PCR. Results: The minimum MIC of allicin against A. fumigatus was found to be 3.2 μg/ml. Scanning electron micrographs exhibited significant morphological alteration of A. fumigatus hyphae and conidia after 48 h incubation at 1× MIC, 2× MIC and sub-MIC concentrations of allicin 1/4× MIC and 1/2× MIC in comparison with untreated (growth) control. Upon treatment at 1/4× MIC (0.8 μg/ml), hyphae were seen as wrinkled with patchy and rough surface implying decrease in structural integrity. Further, at 1/2× MIC (1.6 μg/ml), aberrant shape of hyphae with irregular constriction was observed along the length of hypha. At 1X MIC, complete suppression of hyphae growth was observed and only conidia were visible. Real-time PCR findings revealed that significant down-regulation of chsE, chsG, fksA, and gel2 genes were observed at various MICs of allicin tested. On the contrary, rhoD showed an upregulated expression. Conclusion: This study suggests the ability of allicin to affect the expression of cell wall-related genes of A. fumigatus." @default.
• W3125712789 created "2021-02-01" @default.
• W3125712789 creator A5020055588 @default.
• W3125712789 creator A5041368894 @default.
• W3125712789 creator A5064069133 @default.
• W3125712789 creator A5073944353 @default.
• W3125712789 creator A5089482295 @default.
• W3125712789 date "2020-12-01" @default.
• W3125712789 modified "2023-09-25" @default.
• W3125712789 title "Allicin modulates expression of cell wall-related genes in Aspergillus fumigatus" @default.
• W3125712789 doi "https://doi.org/10.1016/j.ijid.2020.09.1033" @default.
• W3125712789 hasPublicationYear "2020" @default.
• W3125712789 type Work @default.
• W3125712789 sameAs 3125712789 @default.
• W3125712789 citedByCount "1" @default.
• W3125712789 countsByYear W31257127892023 @default.
• W3125712789 crossrefType "journal-article" @default.
• W3125712789 hasAuthorship W3125712789A5020055588 @default.
• W3125712789 hasAuthorship W3125712789A5041368894 @default.
• W3125712789 hasAuthorship W3125712789A5064069133 @default.
• W3125712789 hasAuthorship W3125712789A5073944353 @default.
• W3125712789 hasAuthorship W3125712789A5089482295 @default.
• W3125712789 hasBestOaLocation W31257127891 @default.
• W3125712789 hasConcept C104317684 @default.
• W3125712789 hasConcept C125235067 @default.
• W3125712789 hasConcept C176947019 @default.
• W3125712789 hasConcept C2775839086 @default.
• W3125712789 hasConcept C2779787849 @default.
• W3125712789 hasConcept C2781005338 @default.
• W3125712789 hasConcept C4937899 @default.
• W3125712789 hasConcept C55493867 @default.
• W3125712789 hasConcept C86803240 @default.
• W3125712789 hasConcept C89423630 @default.
• W3125712789 hasConcept C90080823 @default.
• W3125712789 hasConceptScore W3125712789C104317684 @default.
• W3125712789 hasConceptScore W3125712789C125235067 @default.
• W3125712789 hasConceptScore W3125712789C176947019 @default.
• W3125712789 hasConceptScore W3125712789C2775839086 @default.
• W3125712789 hasConceptScore W3125712789C2779787849 @default.
• W3125712789 hasConceptScore W3125712789C2781005338 @default.
• W3125712789 hasConceptScore W3125712789C4937899 @default.
• W3125712789 hasConceptScore W3125712789C55493867 @default.
• W3125712789 hasConceptScore W3125712789C86803240 @default.
• W3125712789 hasConceptScore W3125712789C89423630 @default.
• W3125712789 hasConceptScore W3125712789C90080823 @default.
• W3125712789 hasLocation W31257127891 @default.
• W3125712789 hasOpenAccess W3125712789 @default.
• W3125712789 hasPrimaryLocation W31257127891 @default.
• W3125712789 hasRelatedWork W1588343339 @default.
• W3125712789 hasRelatedWork W2019790325 @default.
• W3125712789 hasRelatedWork W2036410003 @default.
• W3125712789 hasRelatedWork W2043389182 @default.
• W3125712789 hasRelatedWork W2104729326 @default.
• W3125712789 hasRelatedWork W2104995438 @default.
• W3125712789 hasRelatedWork W2130502321 @default.
• W3125712789 hasRelatedWork W2886381655 @default.
• W3125712789 hasRelatedWork W2903969552 @default.
• W3125712789 hasRelatedWork W2960175553 @default.
• W3125712789 hasVolume "101" @default.
• W3125712789 isParatext "false" @default.
• W3125712789 isRetracted "false" @default.
• W3125712789 magId "3125712789" @default.
• W3125712789 workType "article" @default.