FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3126338368> ?p ?o ?g. }

• W3126338368 endingPage "153485" @default.
• W3126338368 startingPage "153485" @default.
• W3126338368 abstract "Icariin (ICA) is a bioactive compound isolated from epimedium-derived flavonoids that modulates bone mesenchymal stem cell osteogenesis and adipogenesis. However, its precise mechanism in this process is unknown. The purpose of this study was to elucidate the role of ICA on human bone mesenchymal stem cell (hBMSC) osteogenesis and adipogenesis by focusing on miR-23a mediated activation of the Wnt/β-catenin signaling pathway. After ICA treatment, hBMSC osteogenesis and adipogenesis were evaluated using alkaline phosphatase staining, an alkaline phosphatase activity assay, Oil Red O staining, and cellular triglyceride levels. Moreover, the mRNA and protein expression levels of osteogenic and adipogenic markers as well as key factors of the Wnt/β-catenin signaling pathway were measured using quantitative reverse transcription polymerase chain reaction and western blotting. Lithium chloride, an activator of the Wnt/β-catenin signaling pathway, was used as a positive control. Finally, to investigate the role of miR-23a in ICA-induced activation of the Wnt/β-catenin signaling pathway, hBMSCs were transfected with miR-23a mimics or a miR-23a inhibitor. ICA significantly promoted hBMSC osteogenic differentiation by upregulating alkaline phosphatase activity and the expression of bone sialoprotein II (BSPII) and runt-related transcription factor-2 (Runx-2). In contrast, ICA inhibited hBMSC adipogenic differentiation by reducing lipid droplet formation and cellular triglyceride levels as well as by downregulating the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) and CCAAT enhancer-binding protein-α (C/EBP-α). ICA mediated its effects on hBMSCs by activating the Wnt/β-catenin signaling pathway. It did so by upregulating β-catenin, low density lipoprotein receptor-related protein 5 (LRP5), and T cell factor 1 (TCF1). Notably, the up-regulation of these proteins was blocked by Dickkopf-related protein 1 (DKK1). Critically, the effects of ICA on hBMSCs were similar to that of the positive control, lithium chloride. Notably, ICA-induced activation of the Wnt/β-catenin signaling pathway was significantly attenuated following miR-23a up-regulation. Conversely, miR-23a downregulation affected hBMSCs in the same manner as ICA; i.e., it activated the Wnt/β-catenin signaling pathway. ICA promotes and inhibits, respectively, hBMSC osteogenesis and adipogenesis via miR-23a-mediated activation of the Wnt/β-catenin signaling pathway." @default.
• W3126338368 created "2021-02-15" @default.
• W3126338368 creator A5023546935 @default.
• W3126338368 creator A5041958396 @default.
• W3126338368 creator A5048178779 @default.
• W3126338368 creator A5071013128 @default.
• W3126338368 creator A5086326013 @default.
• W3126338368 date "2021-05-01" @default.
• W3126338368 modified "2023-10-01" @default.
• W3126338368 title "Icariin stimulates osteogenesis and suppresses adipogenesis of human bone mesenchymal stem cells via miR-23a-mediated activation of the Wnt/β-catenin signaling pathway" @default.
• W3126338368 cites W1162004567 @default.
• W3126338368 cites W1245201416 @default.
• W3126338368 cites W1561040682 @default.
• W3126338368 cites W1979043688 @default.
• W3126338368 cites W1980027477 @default.
• W3126338368 cites W1983794260 @default.
• W3126338368 cites W1993500165 @default.
• W3126338368 cites W1994596418 @default.
• W3126338368 cites W1997812904 @default.
• W3126338368 cites W2004862098 @default.
• W3126338368 cites W2011458493 @default.
• W3126338368 cites W2015858554 @default.
• W3126338368 cites W2030156843 @default.
• W3126338368 cites W2040136779 @default.
• W3126338368 cites W2040884950 @default.
• W3126338368 cites W2043496866 @default.
• W3126338368 cites W2048480904 @default.
• W3126338368 cites W2048675800 @default.
• W3126338368 cites W2052609811 @default.
• W3126338368 cites W2056112402 @default.
• W3126338368 cites W2060211276 @default.
• W3126338368 cites W2062327383 @default.
• W3126338368 cites W2063460304 @default.
• W3126338368 cites W2071937786 @default.
• W3126338368 cites W2091199400 @default.
• W3126338368 cites W2091673549 @default.
• W3126338368 cites W2094057765 @default.
• W3126338368 cites W2105054202 @default.
• W3126338368 cites W2107277218 @default.
• W3126338368 cites W2128624545 @default.
• W3126338368 cites W2128775512 @default.
• W3126338368 cites W2135769482 @default.
• W3126338368 cites W2138924822 @default.
• W3126338368 cites W2148394926 @default.
• W3126338368 cites W2150026598 @default.
• W3126338368 cites W2153720415 @default.
• W3126338368 cites W2154671690 @default.
• W3126338368 cites W2166477100 @default.
• W3126338368 cites W2169021301 @default.
• W3126338368 cites W2213712391 @default.
• W3126338368 cites W2232740985 @default.
• W3126338368 cites W2510656127 @default.
• W3126338368 cites W2536899804 @default.
• W3126338368 cites W2588389969 @default.
• W3126338368 cites W2750945983 @default.
• W3126338368 cites W2767402457 @default.
• W3126338368 cites W2913564338 @default.
• W3126338368 cites W2917851744 @default.
• W3126338368 doi "https://doi.org/10.1016/j.phymed.2021.153485" @default.
• W3126338368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33743412" @default.
• W3126338368 hasPublicationYear "2021" @default.
• W3126338368 type Work @default.
• W3126338368 sameAs 3126338368 @default.
• W3126338368 citedByCount "24" @default.
• W3126338368 countsByYear W31263383682021 @default.
• W3126338368 countsByYear W31263383682022 @default.
• W3126338368 countsByYear W31263383682023 @default.
• W3126338368 crossrefType "journal-article" @default.
• W3126338368 hasAuthorship W3126338368A5023546935 @default.
• W3126338368 hasAuthorship W3126338368A5041958396 @default.
• W3126338368 hasAuthorship W3126338368A5048178779 @default.
• W3126338368 hasAuthorship W3126338368A5071013128 @default.
• W3126338368 hasAuthorship W3126338368A5086326013 @default.
• W3126338368 hasConcept C122927707 @default.
• W3126338368 hasConcept C131075544 @default.
• W3126338368 hasConcept C137620995 @default.
• W3126338368 hasConcept C142724271 @default.
• W3126338368 hasConcept C153911025 @default.
• W3126338368 hasConcept C160160445 @default.
• W3126338368 hasConcept C181199279 @default.
• W3126338368 hasConcept C185592680 @default.
• W3126338368 hasConcept C198826908 @default.
• W3126338368 hasConcept C204787440 @default.
• W3126338368 hasConcept C2776363554 @default.
• W3126338368 hasConcept C2776432469 @default.
• W3126338368 hasConcept C2780685212 @default.
• W3126338368 hasConcept C30145163 @default.
• W3126338368 hasConcept C55493867 @default.
• W3126338368 hasConcept C62478195 @default.
• W3126338368 hasConcept C71924100 @default.
• W3126338368 hasConcept C86803240 @default.
• W3126338368 hasConcept C95444343 @default.
• W3126338368 hasConceptScore W3126338368C122927707 @default.
• W3126338368 hasConceptScore W3126338368C131075544 @default.
• W3126338368 hasConceptScore W3126338368C137620995 @default.
• W3126338368 hasConceptScore W3126338368C142724271 @default.
• W3126338368 hasConceptScore W3126338368C153911025 @default.
• W3126338368 hasConceptScore W3126338368C160160445 @default.

• next