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- W3126673080 abstract "Bacteria have the ability to alter their morphology in order to adapt to changing environments. We have investigated the role of the cell envelope in the development and stress-adaptation strategies of Actinobacteria. Here, we demonstrate how cryo-electron microscopy techniques can be used as efficient tools to study cell envelope of various bacteria. An in-depth study on the Streptomyces cell wall with cryo-electron tomography reveals the structural complexity of the Gram-positive cell wall in apically growing bacteria. Additionally, we report the formation of intracellular membrane accumulations in Streptomyces as a result of exposure to a stress-inducing agent. Furthermore, we studied the ability of the filamentous actinomycete Kitasatospora viridifaciens to extrude wall-deficient cells or S-cells upon exposure to hyperosmotic stress. We characterized the structural alterations associated with S-cell formation using cryo-electron microscopy and reveal that S-cell formation requires cytoskeletal protein FilP in K. viridifaciens. To summarize, this thesis provides new insights in the structural complexity and stress-induced alterations of the bacterial cell envelope." @default.
- W3126673080 created "2021-02-15" @default.
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- W3126673080 date "2020-12-02" @default.
- W3126673080 modified "2023-09-27" @default.
- W3126673080 title "Structural characterization of the cell envelope in Actinobacteria under changing environments" @default.
- W3126673080 hasPublicationYear "2020" @default.
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