FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3126768340> ?p ?o ?g. }

• W3126768340 endingPage "138" @default.
• W3126768340 startingPage "133" @default.
• W3126768340 abstract "The aim of the work was development of a monoclonal antibody-based latex agglutination test to identify the causative agent of melioidosis, and the approbation of a freeze-dried experimental preparation for screening of environmental bacterial isolates in Vietnam. Materials and methods . The carriers of specific antibodies were polyacrolein latex particles with active aldehyde groups on the surface. Typical strains of the causative agents of melioidosis and glanders with a full-fledged antigenic structure, as well as the strains Burkholderia thailandensis, Burkholderia cepacia, Pseudomonas aeruginosa, and Pseudomonas putida were used to control the test specificity. The latex agglutination reaction was carried out on plastic Petri dishes with daily bacterial cultures, from which suspensions were prepared at a concentration of 1–2·109 m.c./ml. The results of the reaction were registered visually for 5–8 min using a 4-cross system against a dark background under lighting. The reaction to 3–4 crosses was recorded as positive. Colonies suspected of belonging to pathogenic Burkholderia from primary inoculations were transferred to L-agar with polymyxin B and grown for 36 hours at (37±1) °C. The species of the selected suspicious colonies was determined by multiplex PCR. Results and discussion . With collection strains, latex test demonstrated high sensitivity agglutinating 97.7 % of B. pseudomallei and all B. mallei strains. At the same time, it was negative with B. thailandensis, B. cepacia, P. aeruginos and P. putida. In microbiological screening of bacterial cultures isolated from environmental objects, the latex test had a diagnostic sensitivity of 89.4 %. Using the latex test at the stage of primary screening, it is possible to significantly reduce the time when processing a lot of samples received for analysis, as well as to reduce the consumption of reagents used at the subsequent stages of identification." @default.
• W3126768340 created "2021-02-15" @default.
• W3126768340 creator A5011063787 @default.
• W3126768340 creator A5019558260 @default.
• W3126768340 creator A5048642025 @default.
• W3126768340 creator A5051091301 @default.
• W3126768340 creator A5053449883 @default.
• W3126768340 date "2021-02-07" @default.
• W3126768340 modified "2023-09-27" @default.
• W3126768340 title "Development of a Latex Agglutination Test for Detecting Pathogenic Burkholderia and its Approbation in the Endemic Regions of Vietnam" @default.
• W3126768340 cites W1529647091 @default.
• W3126768340 cites W1677535439 @default.
• W3126768340 cites W1981275479 @default.
• W3126768340 cites W2020988202 @default.
• W3126768340 cites W2055308117 @default.
• W3126768340 cites W2108000869 @default.
• W3126768340 cites W2126115162 @default.
• W3126768340 cites W2263967633 @default.
• W3126768340 cites W2407415576 @default.
• W3126768340 cites W2612139361 @default.
• W3126768340 cites W2661792473 @default.
• W3126768340 cites W2787986304 @default.
• W3126768340 cites W2801821172 @default.
• W3126768340 cites W2897558095 @default.
• W3126768340 cites W2980779926 @default.
• W3126768340 cites W3013927751 @default.
• W3126768340 cites W4211093779 @default.
• W3126768340 doi "https://doi.org/10.21055/0370-1069-2020-4-133-138" @default.
• W3126768340 hasPublicationYear "2021" @default.
• W3126768340 type Work @default.
• W3126768340 sameAs 3126768340 @default.
• W3126768340 citedByCount "0" @default.
• W3126768340 crossrefType "journal-article" @default.
• W3126768340 hasAuthorship W3126768340A5011063787 @default.
• W3126768340 hasAuthorship W3126768340A5019558260 @default.
• W3126768340 hasAuthorship W3126768340A5048642025 @default.
• W3126768340 hasAuthorship W3126768340A5051091301 @default.
• W3126768340 hasAuthorship W3126768340A5053449883 @default.
• W3126768340 hasBestOaLocation W31267683401 @default.
• W3126768340 hasConcept C159654299 @default.
• W3126768340 hasConcept C203014093 @default.
• W3126768340 hasConcept C2778660310 @default.
• W3126768340 hasConcept C2779110727 @default.
• W3126768340 hasConcept C2779164008 @default.
• W3126768340 hasConcept C2779775996 @default.
• W3126768340 hasConcept C2780855054 @default.
• W3126768340 hasConcept C2781463711 @default.
• W3126768340 hasConcept C37977956 @default.
• W3126768340 hasConcept C523546767 @default.
• W3126768340 hasConcept C54355233 @default.
• W3126768340 hasConcept C86803240 @default.
• W3126768340 hasConcept C89423630 @default.
• W3126768340 hasConceptScore W3126768340C159654299 @default.
• W3126768340 hasConceptScore W3126768340C203014093 @default.
• W3126768340 hasConceptScore W3126768340C2778660310 @default.
• W3126768340 hasConceptScore W3126768340C2779110727 @default.
• W3126768340 hasConceptScore W3126768340C2779164008 @default.
• W3126768340 hasConceptScore W3126768340C2779775996 @default.
• W3126768340 hasConceptScore W3126768340C2780855054 @default.
• W3126768340 hasConceptScore W3126768340C2781463711 @default.
• W3126768340 hasConceptScore W3126768340C37977956 @default.
• W3126768340 hasConceptScore W3126768340C523546767 @default.
• W3126768340 hasConceptScore W3126768340C54355233 @default.
• W3126768340 hasConceptScore W3126768340C86803240 @default.
• W3126768340 hasConceptScore W3126768340C89423630 @default.
• W3126768340 hasIssue "4" @default.
• W3126768340 hasLocation W31267683401 @default.
• W3126768340 hasOpenAccess W3126768340 @default.
• W3126768340 hasPrimaryLocation W31267683401 @default.
• W3126768340 hasRelatedWork W20108125 @default.
• W3126768340 hasRelatedWork W24510054 @default.
• W3126768340 hasRelatedWork W27562378 @default.
• W3126768340 hasRelatedWork W34854735 @default.
• W3126768340 hasRelatedWork W35813781 @default.
• W3126768340 hasRelatedWork W3666292 @default.
• W3126768340 hasRelatedWork W3674970 @default.
• W3126768340 hasRelatedWork W5916641 @default.
• W3126768340 hasRelatedWork W782866 @default.
• W3126768340 hasRelatedWork W41813216 @default.
• W3126768340 isParatext "false" @default.
• W3126768340 isRetracted "false" @default.
• W3126768340 magId "3126768340" @default.
• W3126768340 workType "article" @default.