Matches in SemOpenAlex for { <https://semopenalex.org/work/W3126804597> ?p ?o ?g. }
- W3126804597 endingPage "28" @default.
- W3126804597 startingPage "18" @default.
- W3126804597 abstract "Abstract Objective: Pseudomonas aeruginosa ( P. aeruginosa ) contains a hierarchy of quorum sensing (QS) network, consisting of the las, rhl and pqs systems, which play a key role in coordinating the expression of virulence factors. PA2146 was found to be associated with P. aeruginosa pathogenicity in macrophage and host Immune response. The aim of this study was to investigate the effects of PA2146 on the virulence of P. aeruginosa and explore its mechanism. Methods: PA2146 gene knockout strain and complement strain of P. aeruginosa PAO1 were constructed. The biomass of biofilm was detected by crystal violet staining; the virulence factors were measured, including pyocyanin, rhamnolipid, LasA elastase, LasB elastase and hemolytic activity; RNA-seq and label-free relative quantitative proteomics analyses were carried out to test the influence of PA2146 on transcriptomics and proteomics. This study was approved by the Institutional Review Board of the Third Xiangya Hospital, Central South University, China (approval No. 2019-S021). Results: PA2146 -deficient strains showed reduced biofilm formation and increased pyocyanin, rhamnolipid, LasA elastase, LasB elastase, and hemolytic activity, as well as increased motility, compared with the wild-type strain. RNA-seq and label-free relative quantitative proteomics analyses revealed that PA2146 repressed the transcription of several genes that are integral to the pqs system and to pyocyanin biosynthesis, and increased the expression of MexEF-OprN efflux pump components at the gene and protein level. Conclusion: PA2146 gene was found to inhibit the pqs system. PA2146 may affect quorum sensing by directly inhibiting the pqs system or by enhancing the expression of MexEF-OprN efflux pump components, thereby promoting efflux of 2-heptyl-4(1H)-quinolone, a Pseudomonas quinolone signal precursor, and thus affecting P. aeruginosa virulence." @default.
- W3126804597 created "2021-02-15" @default.
- W3126804597 creator A5019153353 @default.
- W3126804597 creator A5027860565 @default.
- W3126804597 creator A5029837106 @default.
- W3126804597 creator A5032323587 @default.
- W3126804597 creator A5033649826 @default.
- W3126804597 creator A5041240996 @default.
- W3126804597 creator A5052964709 @default.
- W3126804597 creator A5082998428 @default.
- W3126804597 creator A5084419890 @default.
- W3126804597 date "2021-01-28" @default.
- W3126804597 modified "2023-10-12" @default.
- W3126804597 title "PA1426 regulates Pseudomonas aeruginosa quorum sensing and virulence: an in vitro study" @default.
- W3126804597 cites W1932296131 @default.
- W3126804597 cites W1970790687 @default.
- W3126804597 cites W1972010400 @default.
- W3126804597 cites W1974116356 @default.
- W3126804597 cites W1979455969 @default.
- W3126804597 cites W2007002493 @default.
- W3126804597 cites W2027305039 @default.
- W3126804597 cites W2043698490 @default.
- W3126804597 cites W2061972317 @default.
- W3126804597 cites W2062374558 @default.
- W3126804597 cites W2070238977 @default.
- W3126804597 cites W2097936539 @default.
- W3126804597 cites W2102278945 @default.
- W3126804597 cites W2111678991 @default.
- W3126804597 cites W2138207763 @default.
- W3126804597 cites W2161288621 @default.
- W3126804597 cites W2179438025 @default.
- W3126804597 cites W2290216457 @default.
- W3126804597 cites W2306122989 @default.
- W3126804597 cites W2333402398 @default.
- W3126804597 cites W2475881350 @default.
- W3126804597 cites W2588657365 @default.
- W3126804597 cites W2606986624 @default.
- W3126804597 cites W2613356051 @default.
- W3126804597 cites W2615876758 @default.
- W3126804597 cites W2619938970 @default.
- W3126804597 cites W2761529186 @default.
- W3126804597 cites W2771211326 @default.
- W3126804597 cites W2793928847 @default.
- W3126804597 cites W2801850658 @default.
- W3126804597 cites W2810726186 @default.
- W3126804597 cites W2864259052 @default.
- W3126804597 cites W2883707477 @default.
- W3126804597 cites W2900607856 @default.
- W3126804597 cites W2979602430 @default.
- W3126804597 cites W2985476502 @default.
- W3126804597 cites W3012517573 @default.
- W3126804597 cites W3015720474 @default.
- W3126804597 cites W3019067353 @default.
- W3126804597 cites W3032515986 @default.
- W3126804597 cites W3092195624 @default.
- W3126804597 doi "https://doi.org/10.1097/jbr.0000000000000088" @default.
- W3126804597 hasPublicationYear "2021" @default.
- W3126804597 type Work @default.
- W3126804597 sameAs 3126804597 @default.
- W3126804597 citedByCount "1" @default.
- W3126804597 countsByYear W31268045972022 @default.
- W3126804597 crossrefType "journal-article" @default.
- W3126804597 hasAuthorship W3126804597A5019153353 @default.
- W3126804597 hasAuthorship W3126804597A5027860565 @default.
- W3126804597 hasAuthorship W3126804597A5029837106 @default.
- W3126804597 hasAuthorship W3126804597A5032323587 @default.
- W3126804597 hasAuthorship W3126804597A5033649826 @default.
- W3126804597 hasAuthorship W3126804597A5041240996 @default.
- W3126804597 hasAuthorship W3126804597A5052964709 @default.
- W3126804597 hasAuthorship W3126804597A5082998428 @default.
- W3126804597 hasAuthorship W3126804597A5084419890 @default.
- W3126804597 hasBestOaLocation W31268045971 @default.
- W3126804597 hasConcept C104317684 @default.
- W3126804597 hasConcept C118687296 @default.
- W3126804597 hasConcept C181199279 @default.
- W3126804597 hasConcept C185592680 @default.
- W3126804597 hasConcept C2777637488 @default.
- W3126804597 hasConcept C2778549369 @default.
- W3126804597 hasConcept C2781044401 @default.
- W3126804597 hasConcept C2781427627 @default.
- W3126804597 hasConcept C523546767 @default.
- W3126804597 hasConcept C54355233 @default.
- W3126804597 hasConcept C55493867 @default.
- W3126804597 hasConcept C58123911 @default.
- W3126804597 hasConcept C60987743 @default.
- W3126804597 hasConcept C86803240 @default.
- W3126804597 hasConcept C89423630 @default.
- W3126804597 hasConceptScore W3126804597C104317684 @default.
- W3126804597 hasConceptScore W3126804597C118687296 @default.
- W3126804597 hasConceptScore W3126804597C181199279 @default.
- W3126804597 hasConceptScore W3126804597C185592680 @default.
- W3126804597 hasConceptScore W3126804597C2777637488 @default.
- W3126804597 hasConceptScore W3126804597C2778549369 @default.
- W3126804597 hasConceptScore W3126804597C2781044401 @default.
- W3126804597 hasConceptScore W3126804597C2781427627 @default.
- W3126804597 hasConceptScore W3126804597C523546767 @default.
- W3126804597 hasConceptScore W3126804597C54355233 @default.
- W3126804597 hasConceptScore W3126804597C55493867 @default.