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- W3127100265 abstract "Nucleation of microtubules (MTs) is essential for cellular activities, but its mechanism is unknown because of the difficulty involved in capturing rare stochastic events in the early stage of polymerization. Here, combining rapid flush negative stain electron microscopy (EM) and kinetic analysis, we demonstrate that the formation of straight oligomers of critical size is essential for nucleation. Both GDP and GTP tubulin form single-stranded oligomers with a broad range of curvatures, but upon nucleation, the curvature distribution of GTP oligomers is shifted to produce a minor population of straight oligomers. With tubulin having the Y222F mutation in the β subunit, the proportion of straight oligomers increases and nucleation accelerates. Our results support a model in which GTP binding generates a minor population of straight oligomers compatible with lateral association and further growth to MTs. This study suggests that cellular factors involved in nucleation promote it via stabilization of straight oligomers." @default.
- W3127100265 created "2021-02-15" @default.
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- W3127100265 date "2021-02-05" @default.
- W3127100265 modified "2023-10-04" @default.
- W3127100265 title "GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation" @default.
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- W3127100265 cites W1888780705 @default.
- W3127100265 cites W1965404941 @default.
- W3127100265 cites W1970611771 @default.
- W3127100265 cites W1972823922 @default.
- W3127100265 cites W1975198943 @default.
- W3127100265 cites W1978888721 @default.
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- W3127100265 cites W1991948593 @default.
- W3127100265 cites W2002652733 @default.
- W3127100265 cites W2009711715 @default.
- W3127100265 cites W2011521398 @default.
- W3127100265 cites W2012901551 @default.
- W3127100265 cites W2014184691 @default.
- W3127100265 cites W2014383026 @default.
- W3127100265 cites W2014528904 @default.
- W3127100265 cites W2015005644 @default.
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- W3127100265 cites W2076659391 @default.
- W3127100265 cites W2079180616 @default.
- W3127100265 cites W2081406767 @default.
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- W3127100265 cites W2115376659 @default.
- W3127100265 cites W2117931147 @default.
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- W3127100265 cites W2121903259 @default.
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- W3127100265 cites W2130122714 @default.
- W3127100265 cites W2146453988 @default.
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- W3127100265 cites W2170344660 @default.
- W3127100265 cites W2171219367 @default.
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- W3127100265 cites W2519303614 @default.
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- W3127100265 doi "https://doi.org/10.1083/jcb.202007033" @default.
- W3127100265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7871348" @default.
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- W3127100265 hasPublicationYear "2021" @default.
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