Matches in SemOpenAlex for { <https://semopenalex.org/work/W3127111330> ?p ?o ?g. }
- W3127111330 endingPage "2708" @default.
- W3127111330 startingPage "2695" @default.
- W3127111330 abstract "Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is a common molecular imaging modality used to characterise the abundance and spatial distribution of lipids in situ. There are several technical challenges predominantly involving sample pre-treatment and preparation which have complicated the analysis of clinical tissues by MALDI-MSI. Firstly, the common embedding of samples in optimal cutting temperature (O.C.T.), which contains high concentrations of polyethylene glycol (PEG) polymers, causes analyte signal suppression during mass spectrometry (MS) by competing for available ions during ionisation. This suppressive effect has constrained the application of MALDI-MSI for the molecular mapping of clinical tissues. Secondly, the complexity of the mass spectra is obtained by the formation of multiple adduct ions. The process of analyte ion formation during MALDI can generate multiple m/z peaks from a single lipid species due to the presence of alkali salts in tissues, resulting in the suppression of protonated adduct formation and the generation of multiple near isobaric ions which produce overlapping spatial distributions. Presented is a method to simultaneously remove O.C.T. and endogenous salts. This approach was applied to lipid imaging in order to prevent analyte suppression, simplify data interpretation, and improve sensitivity by promoting lipid protonation and reducing the formation of alkali adducts." @default.
- W3127111330 created "2021-02-15" @default.
- W3127111330 creator A5003295517 @default.
- W3127111330 creator A5016018766 @default.
- W3127111330 creator A5019730794 @default.
- W3127111330 creator A5023698490 @default.
- W3127111330 creator A5040319024 @default.
- W3127111330 creator A5050075947 @default.
- W3127111330 creator A5078810319 @default.
- W3127111330 date "2021-02-09" @default.
- W3127111330 modified "2023-10-15" @default.
- W3127111330 title "Removal of optimal cutting temperature (O.C.T.) compound from embedded tissue for MALDI imaging of lipids" @default.
- W3127111330 cites W1973641871 @default.
- W3127111330 cites W1982216518 @default.
- W3127111330 cites W2006126625 @default.
- W3127111330 cites W2007498119 @default.
- W3127111330 cites W2007607168 @default.
- W3127111330 cites W2016360252 @default.
- W3127111330 cites W2018887540 @default.
- W3127111330 cites W2035260685 @default.
- W3127111330 cites W2038768690 @default.
- W3127111330 cites W2039032033 @default.
- W3127111330 cites W2049385065 @default.
- W3127111330 cites W2055312157 @default.
- W3127111330 cites W2055708199 @default.
- W3127111330 cites W2057477884 @default.
- W3127111330 cites W2058448164 @default.
- W3127111330 cites W2064858907 @default.
- W3127111330 cites W2079693268 @default.
- W3127111330 cites W2086213322 @default.
- W3127111330 cites W2089706020 @default.
- W3127111330 cites W2091366926 @default.
- W3127111330 cites W2098540189 @default.
- W3127111330 cites W2101919001 @default.
- W3127111330 cites W2108136373 @default.
- W3127111330 cites W2118995896 @default.
- W3127111330 cites W2122873002 @default.
- W3127111330 cites W2141061440 @default.
- W3127111330 cites W2168283992 @default.
- W3127111330 cites W2177968207 @default.
- W3127111330 cites W2192483331 @default.
- W3127111330 cites W2315348221 @default.
- W3127111330 cites W2413445604 @default.
- W3127111330 cites W2560539177 @default.
- W3127111330 cites W2579741190 @default.
- W3127111330 cites W2624499641 @default.
- W3127111330 cites W2739549819 @default.
- W3127111330 cites W2770676323 @default.
- W3127111330 cites W2774573103 @default.
- W3127111330 cites W2794477889 @default.
- W3127111330 cites W2801368327 @default.
- W3127111330 cites W2885927772 @default.
- W3127111330 cites W2886511294 @default.
- W3127111330 cites W2887931204 @default.
- W3127111330 cites W2888486111 @default.
- W3127111330 cites W2889436730 @default.
- W3127111330 cites W2890050896 @default.
- W3127111330 cites W2891820530 @default.
- W3127111330 cites W2892942916 @default.
- W3127111330 cites W2900465221 @default.
- W3127111330 cites W2913335599 @default.
- W3127111330 cites W2915763066 @default.
- W3127111330 cites W2943315626 @default.
- W3127111330 cites W2943692490 @default.
- W3127111330 cites W2952091990 @default.
- W3127111330 cites W2971524083 @default.
- W3127111330 cites W2980561120 @default.
- W3127111330 cites W2994391268 @default.
- W3127111330 cites W3000891497 @default.
- W3127111330 cites W3020792739 @default.
- W3127111330 cites W3044383543 @default.
- W3127111330 doi "https://doi.org/10.1007/s00216-020-03128-z" @default.
- W3127111330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33564925" @default.
- W3127111330 hasPublicationYear "2021" @default.
- W3127111330 type Work @default.
- W3127111330 sameAs 3127111330 @default.
- W3127111330 citedByCount "17" @default.
- W3127111330 countsByYear W31271113302021 @default.
- W3127111330 countsByYear W31271113302022 @default.
- W3127111330 countsByYear W31271113302023 @default.
- W3127111330 crossrefType "journal-article" @default.
- W3127111330 hasAuthorship W3127111330A5003295517 @default.
- W3127111330 hasAuthorship W3127111330A5016018766 @default.
- W3127111330 hasAuthorship W3127111330A5019730794 @default.
- W3127111330 hasAuthorship W3127111330A5023698490 @default.
- W3127111330 hasAuthorship W3127111330A5040319024 @default.
- W3127111330 hasAuthorship W3127111330A5050075947 @default.
- W3127111330 hasAuthorship W3127111330A5078810319 @default.
- W3127111330 hasBestOaLocation W31271113302 @default.
- W3127111330 hasConcept C101092202 @default.
- W3127111330 hasConcept C10390740 @default.
- W3127111330 hasConcept C106487976 @default.
- W3127111330 hasConcept C108204754 @default.
- W3127111330 hasConcept C113196181 @default.
- W3127111330 hasConcept C145148216 @default.
- W3127111330 hasConcept C150394285 @default.
- W3127111330 hasConcept C162356407 @default.
- W3127111330 hasConcept C162711632 @default.