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- W3128048859 endingPage "665" @default.
- W3128048859 startingPage "637" @default.
- W3128048859 abstract "Macroscopic, ultrastructural, and molecular features-like a ball shape, the presence of starch granules, and the up-regulation of genes involved in carbohydrate metabolism and secondary metabolite biosynthesis-distinguish PT regions within a callus. The modification of the mass of pluripotent cells into de novo shoot bud regeneration is highly relevant to developmental biology and for agriculture and biotechnology. This study deals with protuberances (PT), structures that appear during the organogenic long-term culturing of callus (OC) in kiwifruit. These ball-shaped regions of callus might be considered the first morphological sign of the subsequent shoot bud development. Sections of PT show the regular arrangement of some cells, especially on the surface, in contrast to the regions of OC beyond the PT. The cells of OC possess chloroplasts; however, starch granules were observed only in PTs' plastids. Transcriptomic data revealed unique gene expression for each kind of sample: OC, PT, and PT with visible shoot buds (PT-SH). Higher expression of the gene involved in lipid (glycerol-3-phosphate acyltransferase 5 [GPAT5]), carbohydrate (granule-bound starch synthase 1 [GBSS1]), and secondary metabolite (beta-glucosidase 45 [BGL45]) pathways were detected in PT and could be proposed as the markers of these structures. The up-regulation of the regulatory associated protein of TOR (RAPTOR1) was found in PT-SH. The highest expression of the actinidain gene in leaves from two-year-old regenerated plants suggests that the synthesis of this protein takes place in fully developed organs. The findings indicate that PT and PT-SH are specific structures within OC but have more features in common with callus tissue than with organs." @default.
- W3128048859 created "2021-02-15" @default.
- W3128048859 creator A5016660286 @default.
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- W3128048859 creator A5051067951 @default.
- W3128048859 creator A5067311891 @default.
- W3128048859 creator A5075874093 @default.
- W3128048859 creator A5085385198 @default.
- W3128048859 date "2021-02-05" @default.
- W3128048859 modified "2023-10-15" @default.
- W3128048859 title "Protuberances are organized distinct regions of long-term callus: histological and transcriptomic analyses in kiwifruit" @default.
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