FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3128100426> ?p ?o ?g. }

• W3128100426 endingPage "5927" @default.
• W3128100426 startingPage "5917" @default.
• W3128100426 abstract "Lasso peptides are ribosomally synthesized and post-translationally modified peptide (RiPP) natural products that display a unique lariat-like, threaded conformation. Owing to a locked three-dimensional structure, lasso peptides can be unusually stable toward heat and proteolytic degradation. Some lasso peptides have been shown to bind human cell-surface receptors and exhibit anticancer properties, while others display antibacterial or antiviral activities. All known lasso peptides are produced by bacteria and genome-mining studies indicate that lasso peptides are a relatively prevalent class of RiPPs; however, the discovery, isolation, and characterization of lasso peptides are constrained by the lack of an efficient production system. In this study, we employ a cell-free biosynthesis (CFB) strategy to address longstanding challenges associated with lasso peptide production. We report the successful use of CFB for the formation of an array of sequence-diverse lasso peptides that include known examples as well as a new predicted lasso peptide from Thermobifida halotolerans. We further demonstrate the utility of CFB to rapidly generate and characterize multisite precursor peptide variants to evaluate the substrate tolerance of the biosynthetic pathway. By evaluating more than 1000 randomly chosen variants, we show that the lasso-forming cyclase from the fusilassin pathway is capable of producing millions of sequence-diverse lasso peptides via CFB. These data lay a firm foundation for the creation of large lasso peptide libraries using CFB to identify new variants with unique properties." @default.
• W3128100426 created "2021-02-15" @default.
• W3128100426 creator A5020004066 @default.
• W3128100426 creator A5032826742 @default.
• W3128100426 creator A5034313943 @default.
• W3128100426 creator A5067992334 @default.
• W3128100426 creator A5069664761 @default.
• W3128100426 date "2021-04-06" @default.
• W3128100426 modified "2023-10-10" @default.
• W3128100426 title "Cell-Free Biosynthesis to Evaluate Lasso Peptide Formation and Enzyme–Substrate Tolerance" @default.
• W3128100426 cites W1442130020 @default.
• W3128100426 cites W1587481053 @default.
• W3128100426 cites W1587997563 @default.
• W3128100426 cites W1807215826 @default.
• W3128100426 cites W1911723573 @default.
• W3128100426 cites W1920204344 @default.
• W3128100426 cites W1964450237 @default.
• W3128100426 cites W1967293793 @default.
• W3128100426 cites W1976133651 @default.
• W3128100426 cites W1990562658 @default.
• W3128100426 cites W1995875735 @default.
• W3128100426 cites W2011357172 @default.
• W3128100426 cites W2016146312 @default.
• W3128100426 cites W2018638412 @default.
• W3128100426 cites W2019424589 @default.
• W3128100426 cites W2025177545 @default.
• W3128100426 cites W2032323889 @default.
• W3128100426 cites W2064744296 @default.
• W3128100426 cites W2067120481 @default.
• W3128100426 cites W2071463351 @default.
• W3128100426 cites W2073444711 @default.
• W3128100426 cites W2087367101 @default.
• W3128100426 cites W2097857247 @default.
• W3128100426 cites W2110171246 @default.
• W3128100426 cites W2115734610 @default.
• W3128100426 cites W2116120283 @default.
• W3128100426 cites W2146341019 @default.
• W3128100426 cites W2158266834 @default.
• W3128100426 cites W2162854463 @default.
• W3128100426 cites W2166972934 @default.
• W3128100426 cites W2286914510 @default.
• W3128100426 cites W2316756060 @default.
• W3128100426 cites W2320251714 @default.
• W3128100426 cites W2335399423 @default.
• W3128100426 cites W2335697604 @default.
• W3128100426 cites W2376573086 @default.
• W3128100426 cites W2477942967 @default.
• W3128100426 cites W2531156685 @default.
• W3128100426 cites W2586525814 @default.
• W3128100426 cites W2593189583 @default.
• W3128100426 cites W2621442359 @default.
• W3128100426 cites W2782614921 @default.
• W3128100426 cites W2805232019 @default.
• W3128100426 cites W2890680797 @default.
• W3128100426 cites W2903705457 @default.
• W3128100426 cites W2906866653 @default.
• W3128100426 cites W2908443612 @default.
• W3128100426 cites W2911011185 @default.
• W3128100426 cites W2913020662 @default.
• W3128100426 cites W2941148099 @default.
• W3128100426 cites W2953835026 @default.
• W3128100426 cites W2979595179 @default.
• W3128100426 cites W2989928339 @default.
• W3128100426 cites W2990697904 @default.
• W3128100426 cites W2993784412 @default.
• W3128100426 cites W3000182406 @default.
• W3128100426 cites W3021391052 @default.
• W3128100426 cites W3022478176 @default.
• W3128100426 cites W3044472512 @default.
• W3128100426 cites W3045150108 @default.
• W3128100426 cites W3087348949 @default.
• W3128100426 cites W3102798309 @default.
• W3128100426 cites W631993669 @default.
• W3128100426 doi "https://doi.org/10.1021/jacs.1c01452" @default.
• W3128100426 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8062304" @default.
• W3128100426 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33823110" @default.
• W3128100426 hasPublicationYear "2021" @default.
• W3128100426 type Work @default.
• W3128100426 sameAs 3128100426 @default.
• W3128100426 citedByCount "30" @default.
• W3128100426 countsByYear W31281004262021 @default.
• W3128100426 countsByYear W31281004262022 @default.
• W3128100426 countsByYear W31281004262023 @default.
• W3128100426 crossrefType "journal-article" @default.
• W3128100426 hasAuthorship W3128100426A5020004066 @default.
• W3128100426 hasAuthorship W3128100426A5032826742 @default.
• W3128100426 hasAuthorship W3128100426A5034313943 @default.
• W3128100426 hasAuthorship W3128100426A5067992334 @default.
• W3128100426 hasAuthorship W3128100426A5069664761 @default.
• W3128100426 hasBestOaLocation W31281004262 @default.
• W3128100426 hasConcept C104317684 @default.
• W3128100426 hasConcept C136764020 @default.
• W3128100426 hasConcept C167625842 @default.
• W3128100426 hasConcept C181199279 @default.
• W3128100426 hasConcept C185592680 @default.
• W3128100426 hasConcept C2779281246 @default.
• W3128100426 hasConcept C37616216 @default.
• W3128100426 hasConcept C41008148 @default.

• next