FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3128122994> ?p ?o ?g. }

• W3128122994 endingPage "155" @default.
• W3128122994 startingPage "143" @default.
• W3128122994 abstract "Laryngeal cancer (LC) is one of the common head and neck neoplasms and is characterized by resistance to conventional therapy and poor prognosis. This may result from the presence of cancer stem cells (CSCs), which form a small population in tumors with metastatic potential, high invasive capacity, self-renewal, and differentiation. This study aimed to evaluate the effectiveness of 5-fluorouracil and cisplatin individually, as well as the combination of cetuximab and paclitaxel in a CSC subpopulation separated with biomarkers related to tumoral growth (CD44, CD117, and CD133). addition, expression of TrkB, KRAS, HIF-1α, and VEGF-A genes and proteins related to cell proliferation were evaluated in this subpopulation. The CD44, CD133, and CD117 biomarkers were used to analyze the identification and separation of both subpopulations using FACSAria Fusion. Subpopulations positive for CD44, CD133, and CD117 or lacking these biomarkers were classified as laryngeal cancer stem cells (LCSCs) or laryngeal cancer non-stem cells (non-LCSCs), respectively. Matrigel invasion and colony forming assays were performed to confirm CSC presence. Subpopulations were cultured and exposed to 5-fluorouracil, cisplatin, and cetuximab/paclitaxel drugs for 24 h. Cell proliferation was determined using MTS assay. KRAS and TrkB gene expression levels were evaluated using quantitative real time PCR with TaqMan® Assay in both subpopulations. The non-LCSC subpopulation was considered as the control for relative We found that the LCSC subpopulation demonstrated more resistance to cetuximab and paclitaxel combination chemotherapy when compared with the non-LCSC subpopulation of the cell line. These LCSC subpopulations presented up-regulated expression of KRAS, HIF-1α, and VEGF-A genes and proteins and no TrkB gene expression, but TrkB protein expression was up-regulated in the LC cell line when compared to the non-CSC subpopulation. In conclusion, the combination of CD44, CD133, and CD117 biomarkers has stem cell properties. Moreover, LCSCs, are capable of resisting treatment and present high KRAS, HIF-1α, and VEGF-A gene expression." @default.
• W3128122994 created "2021-02-15" @default.
• W3128122994 creator A5004057788 @default.
• W3128122994 creator A5004716500 @default.
• W3128122994 creator A5008798506 @default.
• W3128122994 creator A5047144286 @default.
• W3128122994 creator A5059782470 @default.
• W3128122994 creator A5062164821 @default.
• W3128122994 creator A5064271523 @default.
• W3128122994 creator A5065546088 @default.
• W3128122994 creator A5071200570 @default.
• W3128122994 creator A5083073919 @default.
• W3128122994 creator A5088274504 @default.
• W3128122994 date "2021-01-15" @default.
• W3128122994 modified "2023-10-18" @default.
• W3128122994 title "Anti-EGFR treatment effects on laryngeal cancer stem cells." @default.
• W3128122994 cites W1874303456 @default.
• W3128122994 cites W1886518861 @default.
• W3128122994 cites W1980818252 @default.
• W3128122994 cites W1992112921 @default.
• W3128122994 cites W2002518231 @default.
• W3128122994 cites W2003434061 @default.
• W3128122994 cites W2007531722 @default.
• W3128122994 cites W2012699604 @default.
• W3128122994 cites W2022631563 @default.
• W3128122994 cites W2028101456 @default.
• W3128122994 cites W2031815172 @default.
• W3128122994 cites W2039943331 @default.
• W3128122994 cites W2042328084 @default.
• W3128122994 cites W2044261750 @default.
• W3128122994 cites W2049895972 @default.
• W3128122994 cites W2050573392 @default.
• W3128122994 cites W2061513340 @default.
• W3128122994 cites W2068864395 @default.
• W3128122994 cites W2074365699 @default.
• W3128122994 cites W2089810753 @default.
• W3128122994 cites W2095184120 @default.
• W3128122994 cites W2099540110 @default.
• W3128122994 cites W2115648576 @default.
• W3128122994 cites W2116057372 @default.
• W3128122994 cites W2117282734 @default.
• W3128122994 cites W2135943905 @default.
• W3128122994 cites W2154747925 @default.
• W3128122994 cites W2168414172 @default.
• W3128122994 cites W2260688567 @default.
• W3128122994 cites W2286060226 @default.
• W3128122994 cites W2335155752 @default.
• W3128122994 cites W2339475403 @default.
• W3128122994 cites W2415902314 @default.
• W3128122994 cites W2463838160 @default.
• W3128122994 cites W2474854223 @default.
• W3128122994 cites W2520492110 @default.
• W3128122994 cites W2546600564 @default.
• W3128122994 cites W2580411187 @default.
• W3128122994 cites W2590586174 @default.
• W3128122994 cites W2592883665 @default.
• W3128122994 cites W2595074215 @default.
• W3128122994 cites W2610225152 @default.
• W3128122994 cites W2620894078 @default.
• W3128122994 cites W2653830772 @default.
• W3128122994 cites W2726807085 @default.
• W3128122994 cites W2743180529 @default.
• W3128122994 cites W2762207309 @default.
• W3128122994 cites W2767025366 @default.
• W3128122994 cites W2781075278 @default.
• W3128122994 cites W2789541171 @default.
• W3128122994 cites W2805059421 @default.
• W3128122994 cites W2884732697 @default.
• W3128122994 cites W2887150021 @default.
• W3128122994 cites W2903704624 @default.
• W3128122994 cites W2989928450 @default.
• W3128122994 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7847503" @default.
• W3128122994 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33527014" @default.
• W3128122994 hasPublicationYear "2021" @default.
• W3128122994 type Work @default.
• W3128122994 sameAs 3128122994 @default.
• W3128122994 citedByCount "5" @default.
• W3128122994 countsByYear W31281229942021 @default.
• W3128122994 countsByYear W31281229942022 @default.
• W3128122994 crossrefType "journal-article" @default.
• W3128122994 hasAuthorship W3128122994A5004057788 @default.
• W3128122994 hasAuthorship W3128122994A5004716500 @default.
• W3128122994 hasAuthorship W3128122994A5008798506 @default.
• W3128122994 hasAuthorship W3128122994A5047144286 @default.
• W3128122994 hasAuthorship W3128122994A5059782470 @default.
• W3128122994 hasAuthorship W3128122994A5062164821 @default.
• W3128122994 hasAuthorship W3128122994A5064271523 @default.
• W3128122994 hasAuthorship W3128122994A5065546088 @default.
• W3128122994 hasAuthorship W3128122994A5071200570 @default.
• W3128122994 hasAuthorship W3128122994A5083073919 @default.
• W3128122994 hasAuthorship W3128122994A5088274504 @default.
• W3128122994 hasConcept C10205521 @default.
• W3128122994 hasConcept C121608353 @default.
• W3128122994 hasConcept C1491633281 @default.
• W3128122994 hasConcept C2776694085 @default.
• W3128122994 hasConcept C2776839831 @default.
• W3128122994 hasConcept C2778239845 @default.
• W3128122994 hasConcept C2779998722 @default.

• next