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- W3128133742 abstract "A facile method was developed to produce porous alginate beads (PABs) with a controllable interconnected porous structure with aqueous two phase (ATPS) emulsions as template for 3D cell culture. ATPS emulsions, containing two biocompatible immiscible aqueous phases of cell/dextran (Dex) mixture and alginate (Alg)/polyethylene glycol (PEG) mixture and stabilized by mPEG-BSA particles, were introduced to form PABs. The pore size of PABs could be controlled by changing the emulsification frequency and the volume ratio between the ATPS emulsions and PEG-Alg solution. Moreover, cells could be directly encapsulated in the interconnected pores due to the excellent biocompatibility of ATPS. HeLa and human liver cancer cells encapsulated in the PABs present stronger cell activity (>95 %), proliferation, and enhanced functions compared with the cells encapsulated in general alginate beads (GABs). It is believed that the PABs is a promising microcarriers for 3D cell culture in vitro." @default.
- W3128133742 created "2021-02-15" @default.
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- W3128133742 date "2021-04-01" @default.
- W3128133742 modified "2023-10-14" @default.
- W3128133742 title "Aqueous two-phase emulsions-templated tailorable porous alginate beads for 3D cell culture" @default.
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- W3128133742 doi "https://doi.org/10.1016/j.carbpol.2021.117702" @default.
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