FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3128151454> ?p ?o ?g. }

• W3128151454 abstract "The retinal pigment epithelium (RPE) undergoes characteristic structural changes and epithelial-mesenchymal transition (EMT) during normal aging, which are exacerbated in age-related macular degeneration (AMD). Although the pathogenic mechanisms of aging and AMD remain unclear, transforming growth factor-β1 (TGF-β1) is known to induce oxidative stress, morphometric changes, and EMT as a senescence-promoting factor. In this study, we examined whether intravitreal injection of TGF-β1 into the mouse eye elicits senescence-like morphological alterations in the RPE and if this can be prevented by suppressing mammalian target of rapamycin complex 1 (mTORC1) or NADPH oxidase (NOX) signaling. We verified that intravitreal TGF-β1-induced stress fiber formation and EMT in RPE cells, along with age-associated morphometric changes, including increased variation in cell size and reduced cell density. In RPE cells, exogenous TGF-β1 increased endogenous expression of TGF-β1 and upregulated Smad3-ERK1/2-mTORC1 signaling, increasing reactive oxygen species (ROS) production and EMT. We demonstrated that inhibition of the mTORC1-NOX4 pathway by pretreatment with 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), an activator of AMP-dependent protein kinase, or GKT137831, a NOX1/4 inhibitor, decreased ROS generation, prevented stress fiber formation, attenuated EMT, and improved the regularity of the RPE structure in vitro and in vivo. These results suggest that intravitreal TGF-β1 injection could be used as a screening model to investigate the aging-related structural and functional changes to the RPE. Furthermore, the regulation of TGF-β-mTORC1-NOX signaling could be a potential therapeutic target for reducing pathogenic alterations in aged RPE and AMD." @default.
• W3128151454 created "2021-02-15" @default.
• W3128151454 creator A5018933458 @default.
• W3128151454 creator A5026303073 @default.
• W3128151454 creator A5043271168 @default.
• W3128151454 creator A5079313764 @default.
• W3128151454 creator A5089754599 @default.
• W3128151454 date "2021-02-09" @default.
• W3128151454 modified "2023-10-18" @default.
• W3128151454 title "Blockade of mTORC1‐NOX signaling pathway inhibits TGF‐β1‐mediated senescence‐like structural alterations of the retinal pigment epithelium" @default.
• W3128151454 cites W1135082898 @default.
• W3128151454 cites W1830885889 @default.
• W3128151454 cites W1960434785 @default.
• W3128151454 cites W1966672647 @default.
• W3128151454 cites W1987901145 @default.
• W3128151454 cites W1999233466 @default.
• W3128151454 cites W2006325126 @default.
• W3128151454 cites W2008533319 @default.
• W3128151454 cites W2017838157 @default.
• W3128151454 cites W2023063954 @default.
• W3128151454 cites W2039002855 @default.
• W3128151454 cites W2053405442 @default.
• W3128151454 cites W2056269088 @default.
• W3128151454 cites W2066593135 @default.
• W3128151454 cites W2066867055 @default.
• W3128151454 cites W2073104926 @default.
• W3128151454 cites W2111274886 @default.
• W3128151454 cites W2115932454 @default.
• W3128151454 cites W2142641037 @default.
• W3128151454 cites W2154903097 @default.
• W3128151454 cites W2198528500 @default.
• W3128151454 cites W2275799577 @default.
• W3128151454 cites W2276308812 @default.
• W3128151454 cites W2414686680 @default.
• W3128151454 cites W2569601267 @default.
• W3128151454 cites W2581382563 @default.
• W3128151454 cites W2738573440 @default.
• W3128151454 cites W2763682406 @default.
• W3128151454 cites W2787277837 @default.
• W3128151454 cites W2793747233 @default.
• W3128151454 cites W2887977772 @default.
• W3128151454 cites W2894888786 @default.
• W3128151454 cites W2913753968 @default.
• W3128151454 cites W2913817601 @default.
• W3128151454 cites W2944982035 @default.
• W3128151454 cites W2963010656 @default.
• W3128151454 cites W2979971613 @default.
• W3128151454 cites W2984504366 @default.
• W3128151454 cites W3035600038 @default.
• W3128151454 cites W3037858919 @default.
• W3128151454 cites W3042429715 @default.
• W3128151454 cites W320198380 @default.
• W3128151454 doi "https://doi.org/10.1096/fj.202001939rr" @default.
• W3128151454 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33559185" @default.
• W3128151454 hasPublicationYear "2021" @default.
• W3128151454 type Work @default.
• W3128151454 sameAs 3128151454 @default.
• W3128151454 citedByCount "6" @default.
• W3128151454 countsByYear W31281514542021 @default.
• W3128151454 countsByYear W31281514542022 @default.
• W3128151454 countsByYear W31281514542023 @default.
• W3128151454 crossrefType "journal-article" @default.
• W3128151454 hasAuthorship W3128151454A5018933458 @default.
• W3128151454 hasAuthorship W3128151454A5026303073 @default.
• W3128151454 hasAuthorship W3128151454A5043271168 @default.
• W3128151454 hasAuthorship W3128151454A5079313764 @default.
• W3128151454 hasAuthorship W3128151454A5089754599 @default.
• W3128151454 hasConcept C118131993 @default.
• W3128151454 hasConcept C170493617 @default.
• W3128151454 hasConcept C185592680 @default.
• W3128151454 hasConcept C2778024200 @default.
• W3128151454 hasConcept C2778468042 @default.
• W3128151454 hasConcept C2780827179 @default.
• W3128151454 hasConcept C522857546 @default.
• W3128151454 hasConcept C529295009 @default.
• W3128151454 hasConcept C54355233 @default.
• W3128151454 hasConcept C55493867 @default.
• W3128151454 hasConcept C62478195 @default.
• W3128151454 hasConcept C86554907 @default.
• W3128151454 hasConcept C86803240 @default.
• W3128151454 hasConcept C95444343 @default.
• W3128151454 hasConcept C98490376 @default.
• W3128151454 hasConceptScore W3128151454C118131993 @default.
• W3128151454 hasConceptScore W3128151454C170493617 @default.
• W3128151454 hasConceptScore W3128151454C185592680 @default.
• W3128151454 hasConceptScore W3128151454C2778024200 @default.
• W3128151454 hasConceptScore W3128151454C2778468042 @default.
• W3128151454 hasConceptScore W3128151454C2780827179 @default.
• W3128151454 hasConceptScore W3128151454C522857546 @default.
• W3128151454 hasConceptScore W3128151454C529295009 @default.
• W3128151454 hasConceptScore W3128151454C54355233 @default.
• W3128151454 hasConceptScore W3128151454C55493867 @default.
• W3128151454 hasConceptScore W3128151454C62478195 @default.
• W3128151454 hasConceptScore W3128151454C86554907 @default.
• W3128151454 hasConceptScore W3128151454C86803240 @default.
• W3128151454 hasConceptScore W3128151454C95444343 @default.
• W3128151454 hasConceptScore W3128151454C98490376 @default.
• W3128151454 hasFunder F4320322103 @default.
• W3128151454 hasFunder F4320322120 @default.
• W3128151454 hasIssue "3" @default.

• next