Matches in SemOpenAlex for { <https://semopenalex.org/work/W3128274325> ?p ?o ?g. }
- W3128274325 abstract "MicroRNAs (miRNAs) are cargo carried by extracellular vesicles (EVs) and are associated with cell-cell interactions. The response to the cellular environment, such as disease states, genetic/metabolic changes, or differences in cell type, highly regulates cargo sorting to EVs. However, morphological features during EV formation and secretion involving miRNA loading are unknown. This study developed a new method of EV loading using cell resealing and reconstituted the elementary miRNA-loading processes. Morphology, secretory response, and cellular uptake ability of EVs obtained from intact and resealed HeLa cells were comparable. Exogenously added soluble factors were introduced into multivesicular endosomes (MVEs) and their subsequent secretion to the extracellular region occurred in resealed HeLa cells. In addition, miRNA transport to MVEs and miRNA encapsulation to EVs followed a distinct pathway regulated by RNA-binding proteins, such as Argonaute and Y-box binding protein 1, depending on miRNA types. Our cell-resealing system can analyze disease-specific EVs derived from disease model cells, where pathological cytosol is introduced into cells. Thus, EV formation in resealed cells can be used not only to create a reconstitution system to give mechanistic insight into EV encapsulation but also for applications such as loading various molecules into EVs and identifying disease-specific EV markers." @default.
- W3128274325 created "2021-02-15" @default.
- W3128274325 creator A5034921892 @default.
- W3128274325 creator A5048937040 @default.
- W3128274325 creator A5049692234 @default.
- W3128274325 date "2021-02-03" @default.
- W3128274325 modified "2023-10-04" @default.
- W3128274325 title "Applications of cell resealing to reconstitute microRNA loading to extracellular vesicles" @default.
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- W3128274325 doi "https://doi.org/10.1038/s41598-021-82452-5" @default.
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